Construct: ORF ccsbBroad304_04178
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008190.1_s304c1, BRDN0000400160
- Derived from:
- ccsbBroadEn_04178
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EDC3 (80153)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04178
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80153 | EDC3 | enhancer of mRNA decapping 3 | NM_001142443.3 | 100% | 100% | |
| 2 | human | 80153 | EDC3 | enhancer of mRNA decapping 3 | NM_001142444.3 | 100% | 100% | |
| 3 | human | 80153 | EDC3 | enhancer of mRNA decapping 3 | NM_001351378.2 | 100% | 100% | |
| 4 | human | 80153 | EDC3 | enhancer of mRNA decapping 3 | NM_025083.5 | 100% | 100% | |
| 5 | human | 80153 | EDC3 | enhancer of mRNA decapping 3 | XM_024450077.1 | 100% | 100% | |
| 6 | human | 80153 | EDC3 | enhancer of mRNA decapping 3 | XM_024450078.1 | 100% | 100% | |
| 7 | human | 80153 | EDC3 | enhancer of mRNA decapping 3 | XM_024450079.1 | 100% | 100% | |
| 8 | human | 80153 | EDC3 | enhancer of mRNA decapping 3 | NM_001351379.2 | 71.4% | 71.4% | 0_1ins435 |
| 9 | human | 80153 | EDC3 | enhancer of mRNA decapping 3 | XM_011522092.3 | 63.9% | 63.9% | 0_1ins549 |
| 10 | mouse | 353190 | Edc3 | enhancer of mRNA decapping 3 | NM_153799.3 | 89.6% | 95.6% | (many diffs) |
| 11 | mouse | 353190 | Edc3 | enhancer of mRNA decapping 3 | XM_011242760.2 | 89.6% | 95.6% | (many diffs) |
| 12 | mouse | 353190 | Edc3 | enhancer of mRNA decapping 3 | XM_006511247.2 | 57% | 61.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1590
- ORF length:
- 1524
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc tacagattgg ctgggaagta ttgtgtccat caattgtgga gatagcttgg 121 gtgtctatca gggaagagtg tcagctgtgg atcaggtcag ccagaccatt tctctcaccc 181 ggcctttcca taatggagtg aagtgtcttg ttccagaagt caccttcagg gcaggtgaca 241 ttacggagtt aaaaattctg gagataccag gacctggaga caaccaacat tttggagacc 301 ttcatcaaac agaattaggc ccctctggtg ctggctgcca agtgggcatc aatcagaatg 361 gcacaggcaa gtttgtcaag aagccagcct cttccagcag tgcccctcag aatatcccta 421 agaggacaga tgtgaagagc caggatgttg ccgtttcccc gcagcagcaa cagtgctcaa 481 agagctatgt cgacaggcac atggaatcct tgagtcagtc caaaagtttc cgtcgtcggc 541 acaactcctg gtcatctagt agcaggcacc caaatcaggc aactcccaag aaaagtggtt 601 taaagaatgg ccagatgaag aataaagatg acgagtgctt cggggatgat attgaggaga 661 tcccagacac agattttgat tttgaaggga acctggctct ttttgacaag gcagctgtgt 721 ttgaggagat tgatacctat gaaaggagaa gtggtacccg ttcccggggc atcccaaatg 781 aaaggcccac tcggtaccgc catgatgaga acatcttgga gtccgagccc attgtctatc 841 gacggatcat agtgccccac aacgtgagca aggagttctg cacggactct ggcctggttg 901 tcccaagtat ttcctatgag ctgcataaaa agctgttgtc cgtggctgag aagcatgggc 961 tgacccttga gcggagactg gagatgacag gtgtgtgtgc cagtcagatg gcactgaccc 1021 tcctcggagg acctaacagg ttgaatccca aaaatgttca ccagaggcct acagtggctc 1081 tactgtgtgg acctcatgtg aagggggctc agggtatcag ctgtggaagg cacctagcca 1141 accatgatgt ccaggtcatc cttttcctgc ccaattttgt caagatgttg gaatctatca 1201 ccaatgagct gtcgctcttc agcaagaccc aaggccaaca agtgtctagc ctcaaagatc 1261 tgcccactag ccctgtggac ctggtcatca actgcctgga ttgccctgag aacgtcttcc 1321 tgcgcgatca accctggtac aaggcagctg tggcctgggc caaccagaac cgggcaccag 1381 tactcagcat agaccctcct gtgcatgaag tcgaacaggg cattgatgcc aaatggtcac 1441 tggcactggg cctgcctctg ccactggggg agcacgcagg ccgtatctat ttgtgcgaca 1501 ttggcattcc ccagcaggtc ttccaggagg tgggcatcaa ctaccactcg ccctttggct 1561 gcaagtttgt tatcccactg cactctgctt acccaacttt cttgtacaaa gtggttggta 1621 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 1681 ggtggcgcgt taagtcgaca atcaacctct ggatta