Construct: ORF ccsbBroad304_04187

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005265.1_s304c1, BRDN0000403433
Derived from:
ccsbBroadEn_04187
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RNF34 (80196)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_04187
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 80196 RNF34 ring finger protein 34 NM_025126.3 100% 100%
2 human 80196 RNF34 ring finger protein 34 NM_194271.2 99.6% 99.4% 1_3delATG;5G>T
3 human 80196 RNF34 ring finger protein 34 XM_024449190.1 99.6% 99.4% 1_3delATG;5G>T
4 human 80196 RNF34 ring finger protein 34 XM_024449191.1 98.1% 98.1% 0_1ins21
5 human 80196 RNF34 ring finger protein 34 XR_001748871.2 50.6% 1_95del;1024_1236del;1425_2203del
6 human 80196 RNF34 ring finger protein 34 NM_001256858.1 48.2% 47.8% 0_1ins567;4_4delAinsTCTTCGTTTC
7 mouse 80751 Rnf34 ring finger protein 34 NM_030564.1 85.7% 88.2% (many diffs)
8 mouse 80751 Rnf34 ring finger protein 34 XM_006530512.2 68.5% 70.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1182
ORF length:
1116
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgaa ggcgggtgcc acgtctatgt gggcttcgtg ctgtgggctg ctgaatgaag
121tcatgggaac tggagctgtc aggggccagc agtcagcatt tgcaggagcc accggtccat
181tcagatttac accaaaccct gagttttcca cctacccacc agcagctacg gaagggccca
241acatagtttg taaagcctgt gggctttcat tttcagtctt tagaaagaag catgtttgct
301gtgactgcaa gaaggatttt tgctccgttt gttcagtctt acaagaaaat ctccgtagat
361gttctacttg tcacttatta caagagacag catttcagcg ccctcagtta atgcgactga
421aggtgaagga cctgcggcag tatctcattc tgagaaatat acccatagat acttgtcgtg
481agaaagaaga cttggtggat ctagtactgt gccatcatgg actaggctct gaggacgaca
541tggacacaag cagtctgaat tcttcaaggt cccagacttc tagctttttt acacgttcgt
601ttttttcaaa ctatacagcc ccctctgcta ctatgtcttc gtttcaggga gagcttatgg
661atggagacca aacatccaga tctggagtgc cggcacaggt acaaagtgaa atcacttcag
721caaacacaga agatgatgat gacgacgatg atgaggatga tgatgatgaa gaagaaaacg
781cagaggatcg gaaccccggg ctctccaagg agagagtgag agcttcactg tctgacttgt
841caagccttga tgatgtggaa ggaatgagcg tgcgccagct gaaggaaatt ctggctcgga
901attttgtcaa ctattctggc tgttgtgaaa aatgggaact ggtagagaaa gtaaaccggt
961tatacaaaga gaatgaagaa aaccaaaagt cctatggcga gcggctgcag ctgcaggatg
1021aggaagacga cagcctgtgt cgcatctgca tggatgccgt catcgactgt gtcctactgg
1081agtgtgggca catggttacc tgcaccaagt gcggcaagcg catgagtgag tgtcccatct
1141gccggcagta tgtggtgcga gccgtgcacg tgttcaagtc ctgcccaact ttcttgtaca
1201aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
1261ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

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