Construct: ORF ccsbBroad304_04187
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005265.1_s304c1, BRDN0000403433
- Derived from:
- ccsbBroadEn_04187
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF34 (80196)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04187
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80196 | RNF34 | ring finger protein 34 | NM_025126.3 | 100% | 100% | |
| 2 | human | 80196 | RNF34 | ring finger protein 34 | NM_194271.2 | 99.6% | 99.4% | 1_3delATG;5G>T |
| 3 | human | 80196 | RNF34 | ring finger protein 34 | XM_024449190.1 | 99.6% | 99.4% | 1_3delATG;5G>T |
| 4 | human | 80196 | RNF34 | ring finger protein 34 | XM_024449191.1 | 98.1% | 98.1% | 0_1ins21 |
| 5 | human | 80196 | RNF34 | ring finger protein 34 | XR_001748871.2 | 50.6% | 1_95del;1024_1236del;1425_2203del | |
| 6 | human | 80196 | RNF34 | ring finger protein 34 | NM_001256858.1 | 48.2% | 47.8% | 0_1ins567;4_4delAinsTCTTCGTTTC |
| 7 | mouse | 80751 | Rnf34 | ring finger protein 34 | NM_030564.1 | 85.7% | 88.2% | (many diffs) |
| 8 | mouse | 80751 | Rnf34 | ring finger protein 34 | XM_006530512.2 | 68.5% | 70.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1182
- ORF length:
- 1116
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa ggcgggtgcc acgtctatgt gggcttcgtg ctgtgggctg ctgaatgaag 121 tcatgggaac tggagctgtc aggggccagc agtcagcatt tgcaggagcc accggtccat 181 tcagatttac accaaaccct gagttttcca cctacccacc agcagctacg gaagggccca 241 acatagtttg taaagcctgt gggctttcat tttcagtctt tagaaagaag catgtttgct 301 gtgactgcaa gaaggatttt tgctccgttt gttcagtctt acaagaaaat ctccgtagat 361 gttctacttg tcacttatta caagagacag catttcagcg ccctcagtta atgcgactga 421 aggtgaagga cctgcggcag tatctcattc tgagaaatat acccatagat acttgtcgtg 481 agaaagaaga cttggtggat ctagtactgt gccatcatgg actaggctct gaggacgaca 541 tggacacaag cagtctgaat tcttcaaggt cccagacttc tagctttttt acacgttcgt 601 ttttttcaaa ctatacagcc ccctctgcta ctatgtcttc gtttcaggga gagcttatgg 661 atggagacca aacatccaga tctggagtgc cggcacaggt acaaagtgaa atcacttcag 721 caaacacaga agatgatgat gacgacgatg atgaggatga tgatgatgaa gaagaaaacg 781 cagaggatcg gaaccccggg ctctccaagg agagagtgag agcttcactg tctgacttgt 841 caagccttga tgatgtggaa ggaatgagcg tgcgccagct gaaggaaatt ctggctcgga 901 attttgtcaa ctattctggc tgttgtgaaa aatgggaact ggtagagaaa gtaaaccggt 961 tatacaaaga gaatgaagaa aaccaaaagt cctatggcga gcggctgcag ctgcaggatg 1021 aggaagacga cagcctgtgt cgcatctgca tggatgccgt catcgactgt gtcctactgg 1081 agtgtgggca catggttacc tgcaccaagt gcggcaagcg catgagtgag tgtcccatct 1141 gccggcagta tgtggtgcga gccgtgcacg tgttcaagtc ctgcccaact ttcttgtaca 1201 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 1261 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta