Construct: ORF ccsbBroad304_04250

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016984.1_s304c1, BRDN0000403821
Derived from:
ccsbBroadEn_04250
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TRMT1L (81627)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_04250
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 81627 TRMT1L tRNA methyltransferase 1 like NM_030934.5 100% 100%
2 human 81627 TRMT1L tRNA methyltransferase 1 like NM_001202423.1 78.7% 78.7% 0_1ins468
3 human 81627 TRMT1L tRNA methyltransferase 1 like XM_017002419.2 72.8% 69% (many diffs)
4 mouse 98685 Trmt1l tRNA methyltransferase 1 like NM_026876.3 85.8% 87.7% (many diffs)
5 mouse 98685 Trmt1l tRNA methyltransferase 1 like XM_006529976.2 83.4% 85.4% (many diffs)
6 mouse 98685 Trmt1l tRNA methyltransferase 1 like XM_017313056.1 52.4% 55.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2265
ORF length:
2199
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga gaatatggcg gaggaggagc tgctgcccct ggagaaggag gaggtggagg
121tggcccaggt ccaggtcccg accccggccc gggactcggc tggggtccca gctccggccc
181cggattcggc tctggactcg gctccgactc cggcctcggc tccagcccca gcccctgccc
241tggcccaggc tccggccctg tccccgtccc tagcctctgc ccctgaggag gctaaaagca
301agagacacat ctcaattcaa aggcagcttg ctgatctaga gaatttagct tttgtaactg
361atggaaattt tgactctgcc agctcattga actcagataa tcttgatgca ggcaacagac
421aggcttgtcc attgtgccct aaggaaaaat tcagagcttg taatagccat aagcttcgtc
481gtcacctcca gaatttacac tggaaagtct cagttgaatt tgaaggttac aggatgtgca
541tctgtcactt accttgtcga ccagtgaaac caaacattat tggagaacag ataaccagta
601aaatgggagc ccattatcat tgtatcattt gttcagcaac aatcaccaga agaactgata
661tgctaggaca tgttaggcgc cacatgaata aaggagagac taaatctagt tatattgcag
721cttccactgc taaaccacct aaggaaattt tgaaagaggc agacacggat gtacaagttt
781gtcccaacta ttctatacct cagaaaacag attcctattt taaccccaaa atgaaactaa
841atcggcagct aatattctgt acattggctg ctttggctga ggaacgaaaa cctttggaat
901gtctagatgc ttttggagcc actgggataa tgggattaca gtgggcaaaa catcttggaa
961atgcagtcaa agttacaatc aatgacttga atgaaaattc tgtgacactg attcaggaaa
1021actgccattt aaacaaattg aaagtggtgg tggacagtaa ggaaaaggaa aagagtgatg
1081atattcttga agaaggagag aaaaatcttg gtaatattaa ggtgaccaaa atggatgcca
1141atgtactgat gcatttgaga tcttttgatt tcatacatct agaccctttt ggaacatcag
1201tgaattatct agattctgca ttcagaaata taagaaacct tggcatagtg tcagtgactt
1261ctacagatat cagttcttta tatgccaagg cacagcatgt tgcccggcgt cactacggat
1321gtaacattgt ccgaactgaa tattacaagg aactagcagc cagaattgtt gtagctgcag
1381tggcaagagc tgcagcccga tgcaacaaag gcatagaagt actgtttgca gtggctctgg
1441aacattttgt gttggtagtt gtgagagttt tgaggggacc tacttcagca gatgaaacag
1501ccaagaagat tcaatacctg atccattgtc agtggtgtga agagagaatt tttcagaagg
1561atggtaatat ggtagaagaa aacccatata gacagctgcc ttgtaactgt catggaagca
1621tgcctggaaa gacagcaata gaacttggac ctctgtggtc aagttccctt ttcaatactg
1681gattcctcaa aagaatgcta tttgaatctc ttcaccatgg tttggatgac attcagaccc
1741taataaagac attaatcttt gaatcagagt gtacgcctca aagtcagttt tcaattcatg
1801catcttcaaa tgtcaacaag caagaagaaa atggtgtatt tattaaaact acagatgaca
1861ccacaacaga taattacatt gcacaaggaa agagaaaaag taatgaaatg atcacaaatt
1921taggcaagaa gcaaaagact gatgtcagta ctgaacatcc tcccttttat tacaacattc
1981acagacacag cattaaagga atgaatatgc caaagttaaa aaagtttttg tgctatttat
2041ctcaagcagg ctttcgagta agccgaactc attttgaccc aatgggtgta cgcacagatg
2101cacctctgat gcagtttaaa tctatccttt taaagtacag cacccccacc tacactggag
2161gacagtcaga aagccatgtc cagtcagcat ctgaagatac agtaactgaa agagttgaaa
2221tgtcagtgaa tgacaaagca gaagcaagtg gctgcagaag atggtatcca actttcttgt
2281acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
2341gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

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