Transcript: Mouse NM_026876.3

Mus musculus tRNA methyltransferase 1 like (Trmt1l), mRNA.

Source:
NCBI, updated 2017-05-21
Taxon:
Mus musculus (mouse)
Gene:
Trmt1l (98685)
Length:
2681
CDS:
230..2416

Additional Resources:

NCBI RefSeq record:
NM_026876.3
NBCI Gene record:
Trmt1l (98685)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_026876.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000295565 CGATGATACCACAATAGATAT pLKO_005 2002 CDS 100% 13.200 18.480 N Trmt1l n/a
2 TRCN0000126966 CGGTGCAACAAAGGTATAGAA pLKO.1 1547 CDS 100% 5.625 7.875 N Trmt1l n/a
3 TRCN0000295563 TGGTAGTTGTGAGGGTCTTAA pLKO_005 1602 CDS 100% 13.200 9.240 N Trmt1l n/a
4 TRCN0000126967 GCCACTGGAATAATGGGATTA pLKO.1 1073 CDS 100% 10.800 7.560 N Trmt1l n/a
5 TRCN0000126968 GCACAGCATCAAAGGGATGAA pLKO.1 2134 CDS 100% 4.950 3.465 N Trmt1l n/a
6 TRCN0000126965 GCCAGTTCATTGAACTCAGAT pLKO.1 533 CDS 100% 4.950 3.465 N Trmt1l n/a
7 TRCN0000288273 GCCAGTTCATTGAACTCAGAT pLKO_005 533 CDS 100% 4.950 3.465 N Trmt1l n/a
8 TRCN0000126964 GTTCTTCTCAGGGTTCTGTAT pLKO.1 2427 3UTR 100% 4.950 3.465 N Trmt1l n/a
9 TRCN0000288207 GTTCTTCTCAGGGTTCTGTAT pLKO_005 2427 3UTR 100% 4.950 3.465 N Trmt1l n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_026876.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04250 pDONR223 100% 85.8% 87.7% None (many diffs) n/a
2 ccsbBroad304_04250 pLX_304 0% 85.8% 87.7% V5 (many diffs) n/a
3 TRCN0000491498 CGCCAGCAGACATGCGTCGCACAT pLX_317 14.3% 85.8% 87.7% V5 (many diffs) n/a
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