Construct: ORF ccsbBroad304_04605
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006744.1_s304c1, BRDN0000410974
- Derived from:
- ccsbBroadEn_04605
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRFAP1 (93621)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04605
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 93621 | MRFAP1 | Morf4 family associated pro... | NM_001272053.1 | 100% | 100% | |
| 2 | human | 93621 | MRFAP1 | Morf4 family associated pro... | NM_033296.2 | 100% | 100% | |
| 3 | human | 114932 | MRFAP1L1 | Morf4 family associated pro... | NM_203462.3 | 88% | 85% | (many diffs) |
| 4 | human | 114932 | MRFAP1L1 | Morf4 family associated pro... | XM_005247932.2 | 88% | 85% | (many diffs) |
| 5 | human | 93621 | MRFAP1 | Morf4 family associated pro... | NM_001272054.1 | 81.1% | 78.7% | 291_292ins72 |
| 6 | mouse | 67568 | Mrfap1 | Morf4 family associated pro... | NM_026242.3 | 84.3% | 85% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 447
- ORF length:
- 381
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcg gcccctggac atcgtcgagc tggcggaacc ggaggaagtg gaggtgctgg 121 agcccgagga ggatttcgag cagtttctgc tcccggtcat caacgagatg cgcgaggaca 181 tcgcgtcgct gacgcgcgag cacgggcggg cgtacctgcg gaaccggagc aagctgtggg 241 agatggacaa tatgctcatc cagatcaaaa cgcaggtgga ggcctcggag gagagcgccc 301 tcaaccacct ccagaacccg ggcgacgcgg ccgagggccg ggcggccaag aggtgcgaga 361 aggccgagga gaaggccaag gagattgcga agatggcaga gatgctggtg gagctggtcc 421 ggcggataga gaagagcgag tcgtcgtgcc caactttctt gtacaaagtg gttggtaagc 481 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 541 ggcgcgttaa gtcgacaatc aacctctgga tta