Construct: ORF ccsbBroad304_04607
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003075.1_s304c1, BRDN0000407993
- Derived from:
- ccsbBroadEn_04607
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TBCK (93627)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04607
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 93627 | TBCK | TBC1 domain containing kinase | NM_001163437.3 | 99.9% | 99.8% | 679C>G |
| 2 | human | 93627 | TBCK | TBC1 domain containing kinase | NM_001163435.3 | 95.5% | 95.5% | 480_596del;796C>G |
| 3 | human | 93627 | TBCK | TBC1 domain containing kinase | NM_001163436.4 | 95.5% | 95.5% | 480_596del;796C>G |
| 4 | human | 93627 | TBCK | TBC1 domain containing kinase | XM_011532417.2 | 95.5% | 95.5% | 480_596del;796C>G |
| 5 | human | 93627 | TBCK | TBC1 domain containing kinase | XM_017008846.1 | 95.5% | 95.5% | 480_596del;796C>G |
| 6 | human | 93627 | TBCK | TBC1 domain containing kinase | XM_024454281.1 | 95.5% | 95.5% | 480_596del;796C>G |
| 7 | human | 93627 | TBCK | TBC1 domain containing kinase | XM_024454282.1 | 95.5% | 95.5% | 480_596del;796C>G |
| 8 | human | 93627 | TBCK | TBC1 domain containing kinase | NM_033115.5 | 94.6% | 88.3% | (many diffs) |
| 9 | human | 93627 | TBCK | TBC1 domain containing kinase | XM_017008847.2 | 91.5% | 91.4% | 480_596del;796C>G;2571_2572ins108 |
| 10 | human | 93627 | TBCK | TBC1 domain containing kinase | XM_017008848.1 | 83.7% | 83.6% | 0_1ins318;162_278del;478C>G |
| 11 | human | 93627 | TBCK | TBC1 domain containing kinase | NM_001290768.2 | 83.3% | 80% | (many diffs) |
| 12 | human | 93627 | TBCK | TBC1 domain containing kinase | XM_017008849.1 | 83.3% | 80% | (many diffs) |
| 13 | human | 93627 | TBCK | TBC1 domain containing kinase | XR_001741354.2 | 75.2% | (many diffs) | |
| 14 | human | 93627 | TBCK | TBC1 domain containing kinase | XR_938800.3 | 73.7% | (many diffs) | |
| 15 | human | 93627 | TBCK | TBC1 domain containing kinase | XR_001741353.2 | 72.4% | (many diffs) | |
| 16 | human | 93627 | TBCK | TBC1 domain containing kinase | XR_002959772.1 | 28.9% | (many diffs) | |
| 17 | mouse | 271981 | Tbck | TBC1 domain containing kinase | NM_001163455.2 | 85.1% | 91.3% | (many diffs) |
| 18 | mouse | 271981 | Tbck | TBC1 domain containing kinase | XM_030252639.1 | 71.3% | 76.3% | (many diffs) |
| 19 | mouse | 271981 | Tbck | TBC1 domain containing kinase | XM_006501553.4 | 68.3% | 70% | (many diffs) |
| 20 | mouse | 271981 | Tbck | TBC1 domain containing kinase | XR_001783696.2 | 34.2% | (many diffs) | |
| 21 | mouse | 271981 | Tbck | TBC1 domain containing kinase | XR_003954329.1 | 32.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2628
- ORF length:
- 2562
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtt tcccctgaag gacgctgaaa tgggagcctt taccttcttt gcctcggctc 121 tgccacatga tgtttgtgga agcaatggac ttcctctcac accaaattcc atcaaaattt 181 tagggcgctt tcaaatcctt aaaaccatca cccatcccag actctgccag tatgtggata 241 tttctagggg aaagcatgaa cgactagtgg tcgtggctga acattgtgaa cgtagtctgg 301 aagacttgct tcgagaaagg aaacctgtga gctgttcaac ggttttgtgt atagcatttg 361 aggttcttca gggcttgcag tatatgaaca aacatggtat agtacacagg gcattgtctc 421 ctcataatat cctgttggac cgaaagggac atattaaatt ggctaaattt ggactttatc 481 acatgacagc tcatggtgat gatgttgatt tcccaatagg gtatccctcg tacttggccc 541 ctgagggaag aaaattattt cagagcttgg atatttctga aagactaaaa tttttgctta 601 ctttggattg tgtagatgac actttaatag ttctggctga agagcatggt tgtttggaca 661 ttataaagga gcttcctgaa actgtgatag atcttttgaa taagtgcctt accttccatc 721 cttctaagag gccaacccca gatgaattaa tgaaggacaa agtattcagt gaggtatcac 781 ctttatatac cccctttacc aaacctgcca gtctgttttc atcttctctg agatgtgctg 841 atttaactct gcctgaggat atcagtcagt tgtgtaaaga tataaataat gattacctgg 901 cagaaagatc tattgaagaa gtgtattacc tttggtgttt ggctggaggt gacttggaga 961 aagagcttgt caacaaggaa atcattcgat ccaaaccacc tatctgcaca ctccccaatt 1021 ttctctttga ggatggtgaa agctttggac aaggtcgaga tagaagctcg cttttagatg 1081 ataccactgt gacattgtcg ttatgccagc taagaaatag attgaaagat gttggtggag 1141 aagcatttta cccattactt gaagatgacc agtctaattt acctcattca aacagcaata 1201 atgagttgtc tgcagctgcc acgctccctt taatcatcag agagaaggat acagagtacc 1261 aactaaatag aattattctc ttcgacaggc tgctaaaggc ttatccatat aaaaaaaacc 1321 aaatctggaa agaagcaaga gttgacattc ctcctcttat gagaggttta acctgggctg 1381 ctcttctggg agttgaggga gctattcatg ccaagtacga tgcaattgat aaagacactc 1441 caattcctac agatagacaa attgaagtgg atattcctcg ctgtcatcag tacgatgaac 1501 tgttatcatc accagaaggt catgcaaaat ttaggcgtgt attaaaagcc tgggtagtgt 1561 ctcatcctga tcttgtgtat tggcaaggtc ttgactcact ttgtgctcca ttcctatatc 1621 taaacttcaa taatgaagcc ttggcttatg catgtatgtc tgcttttatt cccaaatacc 1681 tgtataactt cttcttaaaa gacaactcac atgtaataca agagtatctg actgtcttct 1741 ctcagatgat tgcatttcat gatccagagc tgagtaatca tctcaatgag attggtttca 1801 ttccagatct ctatgccatc ccttggtttc ttaccatgtt tactcatgta tttccactac 1861 acaaaatttt ccacctctgg gataccttac tacttgggaa ttcctctttc ccattctgta 1921 ttggagtagc aattcttcag cagctgcggg accggctttt ggctaatggc tttaatgagt 1981 gtattcttct cttctccgat ttaccagaaa ttgacattga acgctgtgtg agagaatcta 2041 tcaacctgtt ttgttggact cctaaaagtg ctacttacag acagcatgct caacctccaa 2101 agccatcttc tgacagcagt ggaggcagaa gttcggcacc ttatttctct gctgagtgtc 2161 cagatcctcc aaagacagat ctgtcaagag aatccatccc attaaatgac ctgaagtcag 2221 aagtatcacc acggatttca gcagaggacc tgattgactt gtgtgagctc acagtgacag 2281 gccacttcaa aacacccagc aagaaaacaa agtccagtaa accaaagctc ctggtggttg 2341 acatccggaa tagtgaagac tttattcgtg gtcacatttc aggaagcatc aacattccat 2401 tcagtgctgc cttcactgca gaaggggagc ttacccaggg cccttacact gctatgctcc 2461 agaacttcaa agggaaggtc attgtcatcg tggggcatgt ggcaaaacac acagctgagt 2521 ttgcagctca ccttgtgaag atgaaatatc caagaatctg tattctagat ggtggcatta 2581 ataaaataaa gccaacaggc ctcctcacca tcccatctcc tcaaatatgc ccaactttct 2641 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 2701 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta