Construct: ORF ccsbBroad304_04665
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001885.1_s304c1, BRDN0000408300
- Derived from:
- ccsbBroadEn_04665
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OSBPL8 (114882)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04665
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 114882 | OSBPL8 | oxysterol binding protein l... | NM_020841.5 | 100% | 100% | |
| 2 | human | 114882 | OSBPL8 | oxysterol binding protein l... | XM_005268621.5 | 100% | 100% | |
| 3 | human | 114882 | OSBPL8 | oxysterol binding protein l... | NM_001319653.2 | 99.6% | 99.6% | 79_80insCTGTTGTAG |
| 4 | human | 114882 | OSBPL8 | oxysterol binding protein l... | XM_017018769.2 | 99.6% | 99.6% | 79_80insCTGTTGTAG |
| 5 | human | 114882 | OSBPL8 | oxysterol binding protein l... | XM_011537856.3 | 98.5% | 98.5% | 43_81del |
| 6 | human | 114882 | OSBPL8 | oxysterol binding protein l... | XM_011537857.3 | 98.5% | 98.5% | 43_81del |
| 7 | human | 114882 | OSBPL8 | oxysterol binding protein l... | XM_011537855.3 | 98.2% | 98.2% | 43_81del;118_119insCTGTTGTAG |
| 8 | human | 114882 | OSBPL8 | oxysterol binding protein l... | XM_017018768.2 | 97.4% | 97.4% | 1_69del |
| 9 | human | 114882 | OSBPL8 | oxysterol binding protein l... | NM_001003712.2 | 95.2% | 95.2% | 0_1ins126 |
| 10 | human | 114882 | OSBPL8 | oxysterol binding protein l... | NM_001319652.2 | 95.2% | 95.2% | 0_1ins126 |
| 11 | human | 114882 | OSBPL8 | oxysterol binding protein l... | XM_006719224.3 | 95.2% | 95.2% | 0_1ins126 |
| 12 | human | 114882 | OSBPL8 | oxysterol binding protein l... | XM_024448829.1 | 95.2% | 95.2% | 0_1ins126 |
| 13 | human | 114882 | OSBPL8 | oxysterol binding protein l... | NM_001319655.2 | 94.6% | 90.8% | (many diffs) |
| 14 | mouse | 237542 | Osbpl8 | oxysterol binding protein-l... | NM_175489.3 | 89.3% | 96% | (many diffs) |
| 15 | mouse | 237542 | Osbpl8 | oxysterol binding protein-l... | XM_006513637.2 | 89% | 95.7% | (many diffs) |
| 16 | mouse | 237542 | Osbpl8 | oxysterol binding protein-l... | XM_006513638.2 | 85.1% | 88.9% | (many diffs) |
| 17 | mouse | 237542 | Osbpl8 | oxysterol binding protein-l... | NM_001003717.1 | 85.1% | 91.9% | (many diffs) |
| 18 | mouse | 237542 | Osbpl8 | oxysterol binding protein-l... | XM_006513639.2 | 85.1% | 91.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2736
- ORF length:
- 2667
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagggaggt ttggcagatg gagaacctga tcgaacttcg cttcttggtg 121 atagcaaaga tgtccttggg ccatcaactg ttgtagcaaa cagtgacgaa tctcagcttc 181 tgacaccagg aaagatgagt cagcgccaag gaaaagaagc ttatccaacg ccaaccaaag 241 atttgcatca gccatctctt agtccagcaa gtcctcatag ccagggtttt gaaagaggga 301 aggaagatat ttctcaaaat aaagatgaat cttcactttc tatgtcaaag agcaagtctg 361 aatctaaact ttataatggc tcagagaagg acagttcaac ttcaagcaaa ctcacaaaaa 421 aagaatctct taaggtacaa aagaaaaatt accgagaaga aaagaaaaga gccacaaagg 481 agctgctcag tacaatcaca gatccttctg ttattgttat ggctgattgg ttaaagattc 541 gtggtactct aaagagctgg accaagttat ggtgtgtgtt gaaacctggg gtgctactga 601 tctataaaac ccaaaaaaat ggtcagtggg taggaacagt tcttctgaat gcctgtgaaa 661 tcattgaacg tccatcaaaa aaggatggct tttgtttcaa acttttccat cctttggagc 721 aatctatttg ggcagtgaag ggtccaaaag gtgaagcggt tggatccatt actcaaccct 781 tacctagcag ttatttgatc atccgagcta cttcagagtc agatggaagg tgctggatgg 841 atgctttgga gttggctttg aaatgttcta gtcttcttaa acgtacaatg atcagagaag 901 gaaaggaaca tgacctgagc gtttcatcag atagcacaca tgtgactttc tatggcttac 961 tacgtgctaa caatctccac agtggtgata acttccagtt aaatgatagt gaaattgaac 1021 gacaacattt taaggaccaa gatatgtatt ctgataaatc tgataaagaa aatgatcaag 1081 aacatgatga gtctgataat gaggtgatgg ggaaaagtga agaaagtgac acagatacat 1141 cagaaagaca agatgactca tatatcgaac ctgagcctgt tgagccttta aaggagacta 1201 cctacactga acagagccat gaagaacttg gagaggcagg tgaggcttct caaacagaaa 1261 ctgtatctga agaaaacaaa agccttatct ggacactatt gaaacaagtc cgtcctggca 1321 tggacctatc caaggtggtt ctgcctacat ttattttgga accccgttct ttcctggata 1381 aactttcaga ttactactat catgcagatt tcctatctga ggcagctctt gaagaaaatc 1441 cttatttccg tttgaagaaa gtagtgaaat ggtatttgtc aggattctat aaaaagccaa 1501 agggactgaa gaaaccttat aatcctatac ttggcgagac tttccgttgt ttatggattc 1561 atcccagaac aaacagcaaa actttttata ttgctgaaca ggtgtcccat catccaccaa 1621 tatctgcctt ttatgttagt aatcgaaaag atggattttg ccttagcggt agtatcctgg 1681 ctaagtctaa gttttatgga aactcattat ctgcaatatt agagggagaa gcacggttaa 1741 ctttcttgaa tagaggtgaa gattatgtaa tgacaatgcc atacgctcat tgtaaaggaa 1801 ttctttatgg tacaatgaca ctggagcttg gtggaacagt caatattaca tgtcaaaaaa 1861 ctggatacag tgcaatactt gaatttaaac taaagccatt cctagggagt agtgactgtg 1921 ttaatcaaat atcagggaaa cttaaactgg gaaaagaagt cctagctact ttggaaggtc 1981 attgggatag tgaagttttt attactgata aaaagactga taattcagag gttttctgga 2041 atccaacacc tgacattaag caatggagat taataaggca cactgtaaaa tttgaagaac 2101 agggagattt tgaatcagag aaactctggc aacgggtaac tcgagccata aatgccaaag 2161 accaaactga agctacccaa gagaagtatg ttttggaaga agctcaaaga caagctgcca 2221 gggatcggaa aacaaaaaat gaagagtggt cttgcaaatt atttgaactt gatccactca 2281 caggagaatg gcattacaag tttgcagata cccgaccatg ggacccactt aatgatatga 2341 tacagtttga aaaagatggt gttattcaga ccaaagtgaa acatcgtact ccaatggtta 2401 gcgtccccaa aatgaaacat aagccaacca ggcaacagaa gaaagtagca aaaggctatt 2461 cctccccaga acctgacatt caagactcct ctggaagtga agctcaatca gtaaaaccaa 2521 gtacaagaag aaagaaagga atagaactgg gagacattca gagttccatc gaatctataa 2581 aacaaacaca ggaagaaatt aaaagaaata ttatggctct tcgaaatcat ttagtttcaa 2641 gcacaccggc cacggattat tttctgcaac aaaaagacta cttcatcatt ttcctcctga 2701 ttttgcttca agtcataata aacttcatgt tcaagttgcc aactttcttg tacaaagtgg 2761 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 2821 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta