Construct: ORF ccsbBroad304_04972

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012677.1_s304c1, BRDN0000405413
Derived from:
ccsbBroadEn_04972
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TMEM120B (144404)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_04972
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 144404 TMEM120B transmembrane protein 120B NM_001080825.2 100% 100%
2 human 144404 TMEM120B transmembrane protein 120B XM_024448851.1 72.8% 52.8% 551_552ins128;741_742ins148
3 human 144404 TMEM120B transmembrane protein 120B XM_024448852.1 59.6% 54.4% (many diffs)
4 mouse 330189 Tmem120b transmembrane protein 120B NM_001039723.2 88.9% 94.3% (many diffs)
5 mouse 330189 Tmem120b transmembrane protein 120B XM_011248230.2 73.7% 77.8% (many diffs)
6 mouse 330189 Tmem120b transmembrane protein 120B XM_006530390.3 68.8% 74.3% (many diffs)
7 mouse 330189 Tmem120b transmembrane protein 120B XR_001784711.1 50.8% (many diffs)
8 mouse 330189 Tmem120b transmembrane protein 120B XR_387570.3 50.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1086
ORF length:
1017
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gtccgggcag ctggagcgtt gcgagcgcga atggcacgag ctggagggag
121aatttcaaga actgcaggag acgcacagga tctacaagca gaagctggag gagctggctg
181cgctgcagac gctgtgtagc agttccatca gtaagcagaa gaagcacctc aaggacttga
241agcttacact ccagaggtgc aaacgccatg ccagtcggga ggaggcggag ctcgttcagc
301agatggcagc gaacatcaag gagcggcagg acgtcttctt cgacatggag gcctacctgc
361ccaagaagaa cgggctctac ttgaacctgg tcctcggcaa tgtgaacgtg accctcctca
421gcaaccaggc caagttcgcc tacaaggacg aatatgagaa gttcaagctc tacctgacca
481tcatcctgct cctgggtgcc gtggcatgtc gatttgtcct tcactacagg gtgactgacg
541aagtcttcaa cttcctgctg gtgtggtatt actgcaccct gaccattcgg gagagcattc
601tcatcagcaa cggctcaaga attaaaggct ggtgggtgtc tcaccactac gtctccacat
661tcctgtccgg agtgatgctg acctggccta atggacccat ttatcagaag tttcgcaacc
721agttcttagc attttccatt tttcagagct gcgtccagtt cctgcaatat tattaccaga
781ggggctgcct ctaccggctg cgggccctgg gggagaggaa ccacctggat ctcacagtgg
841aagggttcca gtcctggatg tggcggggcc tcacctttct cctgcccttc ctcttctgtg
901gccatttctg gcagctctac aatgccgtca cgctgtttga gctctccagc cacgaggaat
961gcagagaatg gcaggtgttc gtactggcgt tcaccttcct catcctcttc ctcggcaact
1021tcctgaccac gctcaaagtc gtgcatgcca agctccagaa gaacagaggc aagacaaagc
1081agccgttgcc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
1141gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
1201acctctggat ta

Download FASTA (ORF) (Full)

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