Construct: ORF ccsbBroad304_04979
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008085.1_s304c1, BRDN0000398726
- Derived from:
- ccsbBroadEn_04979
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMCO5A (145942)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04979
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 145942 | TMCO5A | transmembrane and coiled-co... | NM_001370735.1 | 99.8% | 100% | 93T>C |
| 2 | human | 145942 | TMCO5A | transmembrane and coiled-co... | NM_152453.3 | 99.8% | 100% | 93T>C |
| 3 | human | 145942 | TMCO5A | transmembrane and coiled-co... | NM_001370736.1 | 95.2% | 95.3% | 1_42del;135T>C |
| 4 | human | 145942 | TMCO5A | transmembrane and coiled-co... | NM_001370737.1 | 81.2% | 74% | (many diffs) |
| 5 | human | 145942 | TMCO5A | transmembrane and coiled-co... | XM_011521262.2 | 80.2% | 74.1% | (many diffs) |
| 6 | human | 145942 | TMCO5A | transmembrane and coiled-co... | NM_001330255.1 | 78.2% | 77.7% | (many diffs) |
| 7 | human | 145942 | TMCO5A | transmembrane and coiled-co... | NR_163584.1 | 69.3% | (many diffs) | |
| 8 | human | 145942 | TMCO5A | transmembrane and coiled-co... | NR_163585.1 | 56.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 930
- ORF length:
- 864
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga aatctcaaga ttggctcagt caaaaagaaa cattatcagt ttgaacatgg 121 accttgaaag ggatacgcag agaatagatg aagcaaacca gaaacttctt ctcaaaatcc 181 aagagaggga agataagatt cagaggctgg aaagtgagat cattcagacg cggggcctgg 241 tggaagatga agagtgggag aaggagaacc gcaccacgat ggaaagggaa agagccttgc 301 aggagctgga ggaagaaaca gccagacttg aaaggaagaa taagacgttg gtccacagta 361 taacagaact tcaacaaaag cttacaagga aatcacaaaa gataaccaat tgtgaacaaa 421 gcagtccaga tggagcccta gaagagacaa aggttaagtt acaacagctg gaagcttcct 481 atgcatgcca agagaaggag ctgctcaagg taatgaagga gtatgcattt gtgacccagc 541 tctgtgaaga tcaagccctc tacataaaga agtaccagga aacgttgaag aaaatagaag 601 aagaactaga ggctctgttc cttgagagag aagtatcaaa actcgtgagc atgaaccctg 661 tggaaaaaga gcataccagc caaaataatg agggtactcc tacccaaaag acagcaagat 721 tattcagtaa aaagattttt tgctgtctct ttttcatcac cctatttttc atcagactgc 781 tgagctacat gttttttcat gtaagattca taaatccaga tctcctcgtc aatgtactgc 841 ccaaggtact gggcaggagc accttgtgga agctcagatg cttcttcttt ccatctctca 901 cacttgagac agaggacatg ttaccccact gcccaacttt cttgtacaaa gtggttggta 961 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 1021 ggtggcgcgt taagtcgaca atcaacctct ggatta