Construct: ORF ccsbBroad304_05086

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014716.1_s304c1, BRDN0000408166
Derived from:
ccsbBroadEn_05086
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CRYGN (155051)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_05086
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 155051 CRYGN crystallin gamma N NM_144727.2 100% 100%
2 human 155051 CRYGN crystallin gamma N XM_005249952.4 80.6% 76.5% (many diffs)
3 human 155051 CRYGN crystallin gamma N NM_001308292.1 60.6% 54.5% (many diffs)
4 human 155051 CRYGN crystallin gamma N XM_017011773.1 60.6% 54.5% (many diffs)
5 human 155051 CRYGN crystallin gamma N XR_002956411.1 39.4% (many diffs)
6 human 155051 CRYGN crystallin gamma N XR_002956412.1 29% (many diffs)
7 human 155051 CRYGN crystallin gamma N NR_131779.1 22.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
615
ORF length:
546
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggcgcagcgc tcggggaaga tcactctcta tgaaggcaag cacttcacag
121ggcagaagct ggaggtcttc ggggactgtg acaacttcca ggaccggggc tttatgaacc
181gagtgaactc catccacgtg gagagcggag cctgggtctg cttcaatcac cccgacttcc
241ggggccagca gttcatcttg gagcacggcg actaccccga cttcttccgc tggaacagcc
301acagtgacca catgggctcc tgtcggcctg taggaatgca cggagaacat ttccgcctag
361aaatcttcga gggttgcaac ttcacgggcc agtgcctgga gttcctggag gacagcccct
421tcctccagag caggggctgg gtcaagaact gtgtgaacac catcaaggtg tacggggacg
481gagcagcatg gagccctaga agcttcggag ctgaggactt ccagctgagc agctctcttc
541aatcagatca aggaccggaa gaggccacca caaaaccagc aacaacccag ccacctttcc
601tgactgcaaa cctcttgcca actttcttgt acaaagtggt tggtaagcct atccctaacc
661ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
721cgacaatcaa cctctggatt a

Download FASTA (ORF) (Full)