Construct: ORF ccsbBroad304_05100
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018369.1_s304c1, BRDN0000410971
- Derived from:
- ccsbBroadEn_05100
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM122B (159090)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05100
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 159090 | FAM122B | family with sequence simila... | NM_001166599.3 | 100% | 100% | |
| 2 | human | 159090 | FAM122B | family with sequence simila... | NM_145284.5 | 99.5% | 99.5% | 439_441delAGC |
| 3 | human | 159090 | FAM122B | family with sequence simila... | NM_001170756.1 | 92.8% | 92.8% | 177_233del |
| 4 | human | 159090 | FAM122B | family with sequence simila... | XM_006724730.3 | 92.5% | 92.5% | 177_233del;496_498delAGC |
| 5 | human | 159090 | FAM122B | family with sequence simila... | XM_011531290.2 | 91.8% | 91.8% | 742_807del |
| 6 | human | 159090 | FAM122B | family with sequence simila... | NM_001331089.1 | 91.4% | 91.4% | 177_245del |
| 7 | human | 159090 | FAM122B | family with sequence simila... | XM_011531288.2 | 91.4% | 91.4% | 439_441delAGC;745_810del |
| 8 | human | 159090 | FAM122B | family with sequence simila... | NM_001331088.1 | 91.1% | 91.1% | 177_245del;508_510delAGC |
| 9 | human | 159090 | FAM122B | family with sequence simila... | XM_011531286.2 | 90.1% | 90.1% | 178_189del;451_453delAGC;757_822del |
| 10 | human | 159090 | FAM122B | family with sequence simila... | NM_001331093.1 | 87.2% | 77% | (many diffs) |
| 11 | human | 159090 | FAM122B | family with sequence simila... | NM_001331094.1 | 86.8% | 76.7% | (many diffs) |
| 12 | human | 159090 | FAM122B | family with sequence simila... | NM_001331091.1 | 85.8% | 75.8% | (many diffs) |
| 13 | human | 159090 | FAM122B | family with sequence simila... | XM_011531285.2 | 85.7% | 85.7% | 177_233del;799_864del |
| 14 | human | 159090 | FAM122B | family with sequence simila... | NM_001331090.1 | 85.5% | 75.5% | (many diffs) |
| 15 | human | 159090 | FAM122B | family with sequence simila... | XM_011531284.2 | 85.4% | 85.4% | 177_233del;496_498delAGC;802_867del |
| 16 | human | 159090 | FAM122B | family with sequence simila... | XM_011531283.2 | 84.5% | 84.5% | 177_245del;811_876del |
| 17 | human | 159090 | FAM122B | family with sequence simila... | XM_011531282.2 | 84.3% | 84.3% | 177_245del;508_510delAGC;814_879del |
| 18 | human | 159090 | FAM122B | family with sequence simila... | NM_001166600.2 | 81% | 71.7% | (many diffs) |
| 19 | human | 159090 | FAM122B | family with sequence simila... | NM_001331092.1 | 80.7% | 71.5% | (many diffs) |
| 20 | human | 159090 | FAM122B | family with sequence simila... | XM_011531292.2 | 79.8% | 70.7% | (many diffs) |
| 21 | human | 159090 | FAM122B | family with sequence simila... | XM_011531291.2 | 79.5% | 70.5% | (many diffs) |
| 22 | human | 159090 | FAM122B | family with sequence simila... | NM_001170757.2 | 70.3% | 52.6% | (many diffs) |
| 23 | mouse | 78755 | Fam122b | family with sequence simila... | NM_001166365.2 | 84.6% | 83.1% | (many diffs) |
| 24 | mouse | 78755 | Fam122b | family with sequence simila... | NM_030167.4 | 84.3% | 82.8% | (many diffs) |
| 25 | mouse | 78755 | Fam122b | family with sequence simila... | XM_011251023.2 | 67.1% | 66.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 807
- ORF length:
- 741
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc tcaggagaaa atggagctgg accttgagcc tgacacatct tatgggggaa 121 ccctgaggag atccagcagc gctcccctaa tccatgggct cagtgacctt tcacaggttt 181 tccaacctta cacacttaga actcggagga atagtacaac aattatgagc cgtcacagcc 241 tggaagaagg cctggatatg gtgaacagag aaactgcaca tgaaagggaa atgcaaacgg 301 caatgcagat aagccaatca tgggatgaga gcttgagcct gagtgacagt gattttgaca 361 agccggagaa attatattct cctaagagaa ttgacttcac tccagtttct ccagcacctt 421 cacccaccag gggattcgga aagatgttcg tgagcagcag tggattgcca ccaagtccag 481 ttcccagtcc aagacgattt tcaaggagaa gtcagagtcc agtcaagtgc attagaccca 541 gtgttcttgg tcctcttaaa agaaaaggtg aaatggagac agaaagtcag cccaagagac 601 tcttccaagg cactaccaat atgttatctc cagatgccgc gcaactgtct gatctcagtt 661 catgttcaga tattttggat ggcagtagta gcagcagtgg cttatcctca gacccgctgg 721 ctaaaggcag cgctaccgca gagtctccag tagcatgctc caattcatgc tcttcgttca 781 tcttgatgga tgatctctca cccaagtgcc caactttctt gtacaaagtg gttggtaagc 841 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 901 ggcgcgttaa gtcgacaatc aacctctgga tta