Construct: ORF ccsbBroad304_05264
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014523.1_s304c1, BRDN0000461633
- Derived from:
- ccsbBroadEn_05264
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PHACTR1 (221692)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05264
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221692 | PHACTR1 | phosphatase and actin regul... | NM_001242648.3 | 100% | 100% | |
| 2 | human | 221692 | PHACTR1 | phosphatase and actin regul... | NM_001322308.2 | 100% | 100% | |
| 3 | human | 221692 | PHACTR1 | phosphatase and actin regul... | NM_001322309.2 | 100% | 100% | |
| 4 | human | 221692 | PHACTR1 | phosphatase and actin regul... | NM_030948.4 | 100% | 100% | |
| 5 | human | 221692 | PHACTR1 | phosphatase and actin regul... | XM_017010459.2 | 96.7% | 96.6% | 1729_1757del;1761_1762insACCGA;1765_1788del |
| 6 | human | 221692 | PHACTR1 | phosphatase and actin regul... | XM_017010460.2 | 96.7% | 96.6% | 1729_1757del;1761_1762insACCGA;1765_1788del |
| 7 | human | 221692 | PHACTR1 | phosphatase and actin regul... | XM_017010461.2 | 96.7% | 96.6% | 1729_1757del;1761_1762insACCGA;1765_1788del |
| 8 | human | 221692 | PHACTR1 | phosphatase and actin regul... | XM_017010462.2 | 96.7% | 96.6% | 1729_1757del;1761_1762insACCGA;1765_1788del |
| 9 | human | 221692 | PHACTR1 | phosphatase and actin regul... | NM_001322310.1 | 89.3% | 89.3% | 662_868del |
| 10 | human | 221692 | PHACTR1 | phosphatase and actin regul... | XM_005248934.3 | 89.3% | 89.3% | 662_868del |
| 11 | human | 221692 | PHACTR1 | phosphatase and actin regul... | XM_017010464.2 | 89.2% | 81% | (many diffs) |
| 12 | human | 221692 | PHACTR1 | phosphatase and actin regul... | XM_017010454.2 | 86.7% | 86.6% | (many diffs) |
| 13 | human | 221692 | PHACTR1 | phosphatase and actin regul... | XM_017010455.2 | 86.7% | 86.6% | (many diffs) |
| 14 | human | 221692 | PHACTR1 | phosphatase and actin regul... | XM_017010456.2 | 86.7% | 86.6% | (many diffs) |
| 15 | human | 221692 | PHACTR1 | phosphatase and actin regul... | XM_017010457.2 | 86.7% | 86.6% | (many diffs) |
| 16 | human | 221692 | PHACTR1 | phosphatase and actin regul... | XM_017010458.2 | 86.4% | 78.3% | (many diffs) |
| 17 | human | 221692 | PHACTR1 | phosphatase and actin regul... | NM_001322311.1 | 84.1% | 84.1% | 0_1ins276 |
| 18 | human | 221692 | PHACTR1 | phosphatase and actin regul... | NM_001322312.1 | 84.1% | 84.1% | 0_1ins276 |
| 19 | human | 221692 | PHACTR1 | phosphatase and actin regul... | XM_017010467.2 | 81.3% | 81.2% | (many diffs) |
| 20 | human | 221692 | PHACTR1 | phosphatase and actin regul... | XM_017010469.2 | 81.3% | 81.2% | (many diffs) |
| 21 | human | 221692 | PHACTR1 | phosphatase and actin regul... | NM_001322314.2 | 80% | 72.9% | (many diffs) |
| 22 | human | 221692 | PHACTR1 | phosphatase and actin regul... | XM_017010452.2 | 77.7% | 70.7% | (many diffs) |
| 23 | human | 221692 | PHACTR1 | phosphatase and actin regul... | NM_001322313.1 | 75.1% | 75.1% | 0_1ins276;386_592del |
| 24 | human | 221692 | PHACTR1 | phosphatase and actin regul... | XM_017010465.2 | 72.9% | 72.7% | (many diffs) |
| 25 | human | 221692 | PHACTR1 | phosphatase and actin regul... | XM_017010466.2 | 72.9% | 72.7% | (many diffs) |
| 26 | mouse | 218194 | Phactr1 | phosphatase and actin regul... | NM_001302635.1 | 89.6% | 96.8% | (many diffs) |
| 27 | mouse | 218194 | Phactr1 | phosphatase and actin regul... | XM_017315482.1 | 89.6% | 96.8% | (many diffs) |
| 28 | mouse | 218194 | Phactr1 | phosphatase and actin regul... | XM_017315483.1 | 89.6% | 96.8% | (many diffs) |
| 29 | mouse | 218194 | Phactr1 | phosphatase and actin regul... | XM_006516905.3 | 80.1% | 86.5% | (many diffs) |
| 30 | mouse | 218194 | Phactr1 | phosphatase and actin regul... | XM_006516906.3 | 80.1% | 86.5% | (many diffs) |
| 31 | mouse | 218194 | Phactr1 | phosphatase and actin regul... | XM_011244395.2 | 80.1% | 86.5% | (many diffs) |
| 32 | mouse | 218194 | Phactr1 | phosphatase and actin regul... | XM_017315484.1 | 80.1% | 78.9% | (many diffs) |
| 33 | mouse | 218194 | Phactr1 | phosphatase and actin regul... | NM_001005740.1 | 75.5% | 81.3% | (many diffs) |
| 34 | mouse | 218194 | Phactr1 | phosphatase and actin regul... | XM_006516907.3 | 71.8% | 71% | (many diffs) |
| 35 | mouse | 218194 | Phactr1 | phosphatase and actin regul... | NM_001302636.1 | 66.8% | 72.4% | (many diffs) |
| 36 | mouse | 218194 | Phactr1 | phosphatase and actin regul... | XM_006516911.3 | 66.8% | 72.4% | (many diffs) |
| 37 | mouse | 218194 | Phactr1 | phosphatase and actin regul... | NM_001005748.2 | 21% | 20% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1809
- ORF length:
- 1740
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggattatccc aaaatggatt attttctgga tgtagagtct gctcacagac 121 tcttggatgt tgagtcagct caaagattct tctacagtca aggagctcaa gctcgccggg 181 cgaccctgct cctgcctccc acattaatgg cggcatcctc ggaggatgat atagaccggc 241 ggcccatccg gagagtgcgc tccaagagcg acacgccgta cctcgcagag gccaggatct 301 cctttaacct gggggcagct gaggaagtgg agaggctggc ggcgatgcgt tctgactccc 361 tcgtcccagg cacccacacc ccacccatcc gcaggagaag taagtttgcc aacctgggaa 421 ggattttcaa gccttggaaa tggaggaaga agaaaagcga aaagttcaaa cacacgtcag 481 cagccctgga aaggaaaata tctatgaggc aaagcagaga agagctgata aagcgaggag 541 tcctgaagga aatctatgat aaagatgggg aactctctat atccaatgaa gaggactccc 601 tagaaaatgg gcagtccctg agctccagcc agctgtctct gcctgccctg tccgaaatgg 661 agccagtccc aatgcccagg gatccctgct catatgaggt gctccaaccg tcagacatca 721 tggatgggcc agatcctggc gcccctgtga aattgccttg tctgccagtg aaactgtcgc 781 ctccgctacc tccaaagaaa gtcatgatct gtatgcccgt gggggggcca gacctctcac 841 tggtgtccta cacagcccag aagagtggcc agcagggtgt ggcccagcac caccacactg 901 tcctgccctc ccagatccag caccagctgc agtacggcag ccacggccag cacctcccct 961 ccaccaccgg ctccctcccc atgcacccct cgggctgcag aatgatagac gagctcaaca 1021 aaacgctggc catgaccatg cagaggctgg aaagctctga gcagcgggtc ccctgttcca 1081 cttcttacca cagctctggg ttgcactcgg gtgatggggt caccaaagca ggacctatgg 1141 gccttccaga aataagacaa gtgccaactg ttgtgattga atgtgatgac aataaagaaa 1201 atgtgcctca tgagtcagac tacgaagact cttcttgcct gtatacaaga gaagaggagg 1261 aagaggagga ggacgaagac gacgacagct cattatacac cagctccctg gccatgaagg 1321 tctgcaggaa ggactcctta gccatcaaac tcagcaacag gccctccaag cgagagctgg 1381 aagaaaagaa catccttccc aggcagacgg atgaggagcg gctggagctg aggcaacaga 1441 ttggcaccaa gctcaccagg cggctgagcc agaggccaac tgcagaggaa ctggaacaga 1501 ggaacatttt gaaacctcgg aatgaacaag aggaacagga ggagaagaga gagatcaaga 1561 ggaggctaac ccgaaagctc agtcaaaggc ccacggtgga agagcttcgg gaaagaaaga 1621 tcctcatccg cttcagtgac tacgtggagg tggctgacgc tcaggactat gaccgcaggg 1681 cagataagcc gtggacccgc ctcaccgctg cagacaaagc tgccatccga aaggagctca 1741 atgaattcaa aagcactgag atggaagttc atgaattgag tagacactta acaaggtttc 1801 accgaccttt gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 1861 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 1921 tcaacctctg gatta