Construct: ORF ccsbBroad304_05351
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007888.1_s304c1, BRDN0000408224
- Derived from:
- ccsbBroadEn_05351
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- P4HA3 (283208)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05351
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 283208 | P4HA3 | prolyl 4-hydroxylase subuni... | NM_182904.5 | 100% | 100% | |
| 2 | human | 283208 | P4HA3 | prolyl 4-hydroxylase subuni... | NM_001288748.2 | 89.3% | 82.8% | 1466_1467insGAATGCA;1626_1812del |
| 3 | human | 283208 | P4HA3 | prolyl 4-hydroxylase subuni... | XR_001747836.1 | 85.3% | 1_43del;1606_1821del;1892_1912del | |
| 4 | human | 283208 | P4HA3 | prolyl 4-hydroxylase subuni... | XR_001747837.1 | 83.3% | 1_43del;1676_1959del | |
| 5 | human | 283208 | P4HA3 | prolyl 4-hydroxylase subuni... | XR_001747838.1 | 77.5% | (many diffs) | |
| 6 | human | 283208 | P4HA3 | prolyl 4-hydroxylase subuni... | XR_001747839.1 | 71.3% | (many diffs) | |
| 7 | human | 283208 | P4HA3 | prolyl 4-hydroxylase subuni... | XR_001747840.1 | 64.8% | 1_43del;1217_1218ins160;1516_2111del | |
| 8 | human | 283208 | P4HA3 | prolyl 4-hydroxylase subuni... | NR_110031.2 | 63.7% | (many diffs) | |
| 9 | mouse | 320452 | P4ha3 | procollagen-proline, 2-oxog... | NM_177161.4 | 87.7% | 90% | (many diffs) |
| 10 | mouse | 320452 | P4ha3 | procollagen-proline, 2-oxog... | XM_006507944.3 | 86.3% | 88.6% | (many diffs) |
| 11 | mouse | 320452 | P4ha3 | procollagen-proline, 2-oxog... | XM_011241831.1 | 82.1% | 73.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1701
- ORF length:
- 1632
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gggtcctggg gcgcggctgg cggcgctgct ggcggtgctg gcgctcggga 121 caggagaccc agaaagggct gcggctcggg gcgacacgtt ctcggcgctg accagcgtgg 181 cgcgcgccct ggcgcccgag cgccggctgc tggggctgct gaggcggtac ctgcgcgggg 241 aggaggcgcg gctgcgggac ctgactagat tctacgacaa ggtactttct ttgcatgagg 301 attcaacaac ccctgtggct aaccctctgc ttgcatttac tctcatcaaa cgcctgcagt 361 ctgactggag gaatgtggta catagtctgg aggccagtga gaacatccga gctctgaagg 421 atggctatga gaaggtggag caagaccttc cagcctttga ggaccttgag ggagcagcaa 481 gggccctgat gcggctgcag gacgtgtaca tgctcaatgt gaaaggcctg gcccgaggtg 541 tctttcagag agtcactggc tctgccatca ctgacctgta cagccccaaa cggctctttt 601 ctctcacagg ggatgactgc ttccaagttg gcaaggtggc ctatgacatg ggggattatt 661 accatgccat tccatggctg gaggaggctg tcagtctctt ccgaggatct tacggagagt 721 ggaagacaga ggatgaggca agtctagaag atgccttgga tcacttggcc tttgcttatt 781 tccgggcagg aaatgtttcg tgtgccctca gcctctctcg ggagtttctt ctctacagcc 841 cagataataa gaggatggcc aggaatgtct tgaaatatga aaggctcttg gcagagagcc 901 ccaaccacgt ggtagctgag gctgtcatcc agaggcccaa tataccccac ctgcagacca 961 gagacaccta cgaggggcta tgtcagaccc tgggttccca gcccactctc taccagatcc 1021 ctagcctcta ctgttcctat gagaccaatt ccaacgccta cctgctgctc cagcccatcc 1081 ggaaggaggt catccacctg gagccctaca ttgctctcta ccatgacttc gtcagtgact 1141 cagaggctca gaaaattaga gaacttgcag aaccatggct acagaggtca gtggtggcat 1201 caggggagaa gcagttacaa gtggagtacc gcatcagcaa aagtgcctgg ctgaaggaca 1261 ctgttgaccc aaaactggtg accctcaacc accgcattgc tgccctcaca ggccttgatg 1321 tccggcctcc ctatgcagag tatctgcagg tggtgaacta tggcatcgga ggacactatg 1381 agcctcactt tgaccatgct acgtcaccaa gcagccccct ctacagaatg aagtcaggaa 1441 accgagttgc aacatttatg atctatctga gctcggtgga agctggagga gccacagcct 1501 tcatctatgc caacctcagc gtgcctgtgg ttaggaatgc agcactgttt tggtggaacc 1561 tgcacaggag tggtgaaggg gacagtgaca cacttcatgc tggctgtcct gtcctggtgg 1621 gagataagtg ggtggccaac aagtggatac atgagtatgg acaggaattc cgcagaccct 1681 gcagctccag ccctgaagac ttgccaactt tcttgtacaa agtggttggt aagcctatcc 1741 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 1801 ttaagtcgac aatcaacctc tggatta