Construct: ORF ccsbBroad304_05833
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004821.1_s304c1, BRDN0000409023
- Derived from:
- ccsbBroadEn_05833
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- APEH (327)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05833
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 327 | APEH | acylaminoacyl-peptide hydro... | NM_001640.4 | 99.9% | 100% | 852T>C |
| 2 | human | 327 | APEH | acylaminoacyl-peptide hydro... | XM_024453501.1 | 96.3% | 96.4% | 852T>C;1522_1602del |
| 3 | human | 327 | APEH | acylaminoacyl-peptide hydro... | XM_005265097.2 | 93.6% | 93.7% | 604_605ins138;714T>C |
| 4 | human | 327 | APEH | acylaminoacyl-peptide hydro... | XM_024453503.1 | 90.3% | 90.3% | 604_605ins138;714T>C;1384_1464del |
| 5 | human | 327 | APEH | acylaminoacyl-peptide hydro... | XM_024453500.1 | 87.3% | 79.8% | (many diffs) |
| 6 | human | 327 | APEH | acylaminoacyl-peptide hydro... | XM_011533658.3 | 87.1% | 80.6% | (many diffs) |
| 7 | human | 327 | APEH | acylaminoacyl-peptide hydro... | XM_005265098.4 | 86.1% | 86.2% | 0_1ins303;549T>C |
| 8 | human | 327 | APEH | acylaminoacyl-peptide hydro... | XM_024453499.1 | 84.2% | 78% | (many diffs) |
| 9 | human | 327 | APEH | acylaminoacyl-peptide hydro... | XM_024453504.1 | 83% | 83.1% | 0_1ins303;549T>C;1219_1299del |
| 10 | human | 327 | APEH | acylaminoacyl-peptide hydro... | XM_017006285.1 | 81.3% | 75% | (many diffs) |
| 11 | human | 327 | APEH | acylaminoacyl-peptide hydro... | XM_017006286.1 | 79.8% | 79.9% | 0_1ins303;301_302ins138;411T>C |
| 12 | human | 327 | APEH | acylaminoacyl-peptide hydro... | XM_024453502.1 | 78.6% | 72.5% | (many diffs) |
| 13 | human | 327 | APEH | acylaminoacyl-peptide hydro... | XM_011533661.2 | 73.3% | 72.1% | 0_1ins421;20_21ins164;267T>C |
| 14 | human | 327 | APEH | acylaminoacyl-peptide hydro... | XM_011533662.2 | 73.3% | 72.1% | 0_1ins421;20_21ins164;267T>C |
| 15 | human | 327 | APEH | acylaminoacyl-peptide hydro... | XM_011533663.2 | 73.3% | 72.1% | 0_1ins421;20_21ins164;267T>C |
| 16 | human | 327 | APEH | acylaminoacyl-peptide hydro... | XM_024453505.1 | 70.7% | 69.5% | (many diffs) |
| 17 | human | 327 | APEH | acylaminoacyl-peptide hydro... | XM_024453506.1 | 70.7% | 69.5% | (many diffs) |
| 18 | human | 327 | APEH | acylaminoacyl-peptide hydro... | XM_024453507.1 | 70.7% | 69.5% | (many diffs) |
| 19 | mouse | 235606 | Apeh | acylpeptide hydrolase | NM_146226.2 | 89.1% | 91.3% | (many diffs) |
| 20 | mouse | 235606 | Apeh | acylpeptide hydrolase | XM_030244343.1 | 76.6% | 78.1% | (many diffs) |
| 21 | mouse | 235606 | Apeh | acylpeptide hydrolase | XM_006511723.4 | 71.5% | 71.9% | (many diffs) |
| 22 | mouse | 235606 | Apeh | acylpeptide hydrolase | XM_030244344.1 | 71.5% | 71.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2262
- ORF length:
- 2196
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga acgtcaggtg ctgctgagcg agcccgagga ggcggcggct ctgtatcggg 121 gccttagccg ccagcccgcg ctgagcgccg cctgcctggg cccggaggtc accacgcagt 181 acggcggcca ataccggacg gtgcacactg agtggaccca gagggacctg gaacgcatgg 241 agaacattcg attctgccgc caatacctgg tgttccatga cggggactca gtggtgtttg 301 caggacctgc aggcaacagt gtggagaccc ggggggaact gctgagcaga gagtctcctt 361 caggcaccat gaaagctgtg ctgcgcaagg ctggaggcac gggccctggg gaagagaagc 421 agttcctgga ggtctgggag aagaaccgga agctcaagag cttcaacctg tcagcgctgg 481 agaaacatgg gcctgtttat gaggatgact gctttggctg cctgtcctgg tcgcactcgg 541 agacacactt gttgtatgtg gcagagaaga agcgccccaa ggccgagtcc ttctttcaga 601 ccaaagcctt ggacgtcagt gccagcgatg atgagatagc caggctgaag aagccagacc 661 aagccatcaa gggggatcag tttgtgtttt atgaagactg gggagaaaac atggtttcca 721 aaagcatccc tgtgctctgc gtgctggatg tcgagagtgg caacatctct gtgcttgagg 781 gggtccctga gaatgtgtcc cctggacagg cattttgggc ccctggagat gctggtgtgg 841 tgtttgtggg ctggtggcat gagcccttcc ggttgggcat ccgcttttgc accaatcgca 901 ggtcagccct gtattacgtg gacctcatcg gggggaagtg tgagctcctc tcggatgact 961 ccctggctgt ctcttctccc cggctgagcc cagaccaatg tcgcattgtc tacctgcagt 1021 acccatctct gatcccccat caccaatgca gccagctgtg cctgtatgac tggtatacca 1081 aggttacctc agtggtggta gatgttgtgc ctcggcagct gggagagaac ttctctggga 1141 tctactgcag ccttctgcct ttgggatgct ggtcagctga cagccagaga gtggtctttg 1201 actcggctca gcgcagccgg caggacctgt ttgctgtgga cacccaagtg ggcactgtga 1261 cctccctcac agctggaggg tcaggtggga gctggaagtt gctcacaatt gaccaggacc 1321 tcatggtggc acagttttcc acacccagcc tacctccaac cctgaaagtt gggttcctgc 1381 cttctgcagg gaaggagcag tcagtgttgt gggtgtccct ggaggaggcc gagcccattc 1441 ccgacatcca ctggggcatc cgggtgctac agccaccccc agagcaagag aatgtgcagt 1501 atgctggcct tgactttgaa gcaatcctgc tgcagcctgg cagccctcca gataagaccc 1561 aagtgcccat ggtggtcatg ccccacgggg ggccccattc atcctttgtc actgcctgga 1621 tgctgttccc agccatgctt tgcaagatgg gctttgcggt actactagtg aactatcgtg 1681 gctccacggg ctttggccag gacagcatcc tctccctccc aggcaatgtg ggccaccagg 1741 atgtgaagga tgtccagttt gcagtggaac aggtgctcca ggaggaacac tttgatgcaa 1801 gccatgtggc ccttatgggt ggttcccatg gtggcttcat ttcctgccac ttgattggtc 1861 agtacccaga gacctacagg gcctgcgtgg cccggaaccc cgtgatcaac atcgcctcca 1921 tgttgggctc cactgacatc cctgactggt gcgtggtgga ggctggcttt cctttcagca 1981 gtgactgcct gccagacctc agcgtgtggg ctgagatgct ggacaaatcg cccatcagat 2041 acatccctca ggtgaagaca ccactgttac tgatgttggg ccaggaggac cggcgtgtgc 2101 ccttcaagca gggcatggag tattaccgtg ccctcaagac ccggaatgtg cctgttcggc 2161 tcctgctcta tcccaaaagc acccacgcat tatcagaggt ggaggtggag tcagacagct 2221 tcatgaatgc tgtgctctgg ctacgcacac acttgggcag ctgcccaact ttcttgtaca 2281 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 2341 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta