Transcript: Human XM_017006286.1

PREDICTED: Homo sapiens acylaminoacyl-peptide hydrolase (APEH), transcript variant X11, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
APEH (327)
Length:
2114
CDS:
195..1952

Additional Resources:

NCBI RefSeq record:
XM_017006286.1
NBCI Gene record:
APEH (327)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017006286.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000046692 GAACACTTTGATGCAAGCCAT pLKO.1 1473 CDS 100% 2.640 3.696 N APEH n/a
2 TRCN0000291381 GAACACTTTGATGCAAGCCAT pLKO_005 1473 CDS 100% 2.640 3.696 N APEH n/a
3 TRCN0000046688 CCTGTATTATGTGGACCTCAT pLKO.1 596 CDS 100% 4.050 2.835 N APEH n/a
4 TRCN0000291378 CCTGTATTATGTGGACCTCAT pLKO_005 596 CDS 100% 4.050 2.835 N APEH n/a
5 TRCN0000046690 CGAGTCCTTCTTTCAGACCAA pLKO.1 410 CDS 100% 2.640 1.848 N APEH n/a
6 TRCN0000291379 CGAGTCCTTCTTTCAGACCAA pLKO_005 410 CDS 100% 2.640 1.848 N APEH n/a
7 TRCN0000046689 GCGGTACTACTAGTGAACTAT pLKO.1 1344 CDS 100% 0.000 0.000 N APEH n/a
8 TRCN0000291380 GCGGTACTACTAGTGAACTAT pLKO_005 1344 CDS 100% 0.000 0.000 N APEH n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017006286.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05833 pDONR223 100% 79.8% 79.9% None 0_1ins303;301_302ins138;411T>C n/a
2 ccsbBroad304_05833 pLX_304 0% 79.8% 79.9% V5 0_1ins303;301_302ins138;411T>C n/a
3 TRCN0000471424 ATGGGTTCAGTGATTCTGTGGCCA pLX_317 19.3% 79.8% 79.9% V5 0_1ins303;301_302ins138;411T>C n/a
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