Construct: ORF ccsbBroad304_05872

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000799.1_s304c1, BRDN0000403900
Derived from:
ccsbBroadEn_05872
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ATP6V1B1 (525)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_05872
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 525 ATP6V1B1 ATPase H+ transporting V1 s... NM_001692.4 99.8% 100% 138C>T;1002C>T
2 human 525 ATP6V1B1 ATPase H+ transporting V1 s... XM_011532907.2 88.7% 84.4% (many diffs)
3 mouse 110935 Atp6v1b1 ATPase, H+ transporting, ly... NM_134157.2 87.4% 93.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1605
ORF length:
1539
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc catggagata gacagcaggc ctggggggct ccccggcagt agctgcaacc
121taggtgcagc ccgagaacac atgcaggcgg tcacccgaaa ctacatcacc cacccccgtg
181tcacctacag gactgtgtgc agtgtgaacg ggcccctggt ggtgctggac cgggtcaagt
241ttgcccagta tgcggagatc gtccacttca ccctcccaga tgggactcag aggagcgggc
301aggtgcttga ggtggctggc accaaggcga ttgttcaggt gtttgaaggg acatcaggga
361tcgatgccag gaagaccact tgcgaattta caggggacat cctacgaact ccggtgtcag
421aggacatgct gggtcgggtt ttcaatggct ccggcaagcc cattgacaag gggccagtgg
481tcatggcgga ggactttctg gatatcaatg gccagcccat caacccgcac tcccgcatct
541accccgagga gatgattcag acgggcattt ctcctattga cgtcatgaac agcattgccc
601gcggccagaa gatccccatc ttctcagcag ccgggctccc ccacaatgag attgccgctc
661agatctgccg ccaggcgggg ctggtgaaga agtccaaggc tgtgctggat taccatgacg
721acaacttcgc catcgtcttt gcagccatgg gggtgaacat ggagacagcc agattcttca
781agtctgactt tgagcagaat ggaaccatgg ggaacgtctg cctcttcctg aacttggcca
841atgaccccac gatcgagcgg atcatcaccc cgcgcctggc gctgaccact gctgaattcc
901ttgcctacca gtgtgagaag catgtgctgg tcatactgac ggacatgagt tcctatgcag
961aggccttgcg ggaggtctct gctgctagag aggaggtgcc tgggcgccga gggtttcctg
1021gatatatgta cacagacctg gccaccatct acgagcgggc gggccgtgtg gagggtcggg
1081gaggatccat cacacagatc cccatcctca ccatgcccaa cgacgatatc acccacccta
1141tcccagactt gacgggcttc atcacagagg gacagatcta cgtggacaga cagcttcaca
1201acagacagat ctaccccccc atcaacgtgc tcccttccct gtcgcggctg atgaagtcag
1261ccattgggga aggcatgaca agaaaggacc atggagatgt ctccaaccag ctgtacgcct
1321gctatgccat cgggaaggac gtgcaggcca tgaaggcagt agttggggag gaggcgctca
1381cctctgagga cctgctctac ctggaattcc tgcagaagtt tgagaagaac ttcatcaatc
1441agggccccta cgagaaccgc tcggtgttcg agtcgctgga cctgggctgg aagctgctgc
1501gcatcttccc caaggagatg ctgaagcgca ttccgcaggc cgtgatcgac gagttctatt
1561cccgcgaggg ggcgctgcag gacctcgcgc ctgacactgc gctctaccca actttcttgt
1621acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
1681gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

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