Construct: ORF ccsbBroad304_05897
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001312.1_s304c1, BRDN0000462266
- Derived from:
- ccsbBroadEn_05897
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BMP1 (649)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05897
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 649 | BMP1 | bone morphogenetic protein 1 | NM_006129.5 | 99.9% | 99.7% | 2155G>A;2313G>T |
| 2 | human | 649 | BMP1 | bone morphogenetic protein 1 | NR_033403.2 | 81.4% | (many diffs) | |
| 3 | human | 649 | BMP1 | bone morphogenetic protein 1 | NR_033404.2 | 77.2% | (many diffs) | |
| 4 | human | 649 | BMP1 | bone morphogenetic protein 1 | NM_001199.4 | 72.8% | 70.5% | (many diffs) |
| 5 | mouse | 12153 | Bmp1 | bone morphogenetic protein 1 | NM_009755.3 | 88.6% | 95.2% | (many diffs) |
| 6 | mouse | 12153 | Bmp1 | bone morphogenetic protein 1 | XM_006518460.3 | 88.6% | 95.2% | (many diffs) |
| 7 | mouse | 12153 | Bmp1 | bone morphogenetic protein 1 | XM_017315805.1 | 77.3% | 82.7% | (many diffs) |
| 8 | mouse | 12153 | Bmp1 | bone morphogenetic protein 1 | XM_011244931.1 | 73.5% | 79.6% | (many diffs) |
| 9 | mouse | 12153 | Bmp1 | bone morphogenetic protein 1 | XR_001781089.1 | 73.1% | (many diffs) | |
| 10 | mouse | 12153 | Bmp1 | bone morphogenetic protein 1 | XM_011244932.2 | 67.4% | 67.5% | (many diffs) |
| 11 | mouse | 12153 | Bmp1 | bone morphogenetic protein 1 | XM_011244933.2 | 66.2% | 68.6% | (many diffs) |
| 12 | mouse | 12153 | Bmp1 | bone morphogenetic protein 1 | XR_001781090.1 | 61.2% | (many diffs) | |
| 13 | mouse | 12153 | Bmp1 | bone morphogenetic protein 1 | XR_383136.2 | 59.7% | (many diffs) | |
| 14 | mouse | 12153 | Bmp1 | bone morphogenetic protein 1 | NR_033241.1 | 59.1% | (many diffs) | |
| 15 | mouse | 12153 | Bmp1 | bone morphogenetic protein 1 | XR_001781091.1 | 57.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3027
- ORF length:
- 2958
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcccggcgtg gcccgcctgc cgctgctgct cgggctgctg ctgctcccgc 121 gtcccggccg gccgctggac ttggccgact acacctatga cctggcggag gaggacgact 181 cggagcccct caactacaaa gacccctgca aggcggctgc ctttcttggg gacattgccc 241 tggacgaaga ggacctgagg gccttccagg tacagcaggc tgtggatctc agacggcaca 301 cagctcgtaa gtcctccatc aaagctgcag ttccaggaaa cacttctacc cccagctgcc 361 agagcaccaa cgggcagcct cagaggggag cctgtgggag atggagaggt agatcccgta 421 gccggcgggc ggcgacgtcc cgaccagagc gtgtgtggcc cgatggggtc atcccctttg 481 tcattggggg aaacttcact ggtagccaga gggcagtctt ccggcaggcc atgaggcact 541 gggagaagca cacctgtgtc accttcctgg agcgcactga cgaggacagc tatattgtgt 601 tcacctatcg accttgcggg tgctgctcct acgtgggtcg ccgcggcggg ggcccccagg 661 ccatctccat cggcaagaac tgtgacaagt tcggcattgt ggtccacgag ctgggccacg 721 tcgtcggctt ctggcacgaa cacactcggc cagaccggga ccgccacgtt tccatcgttc 781 gtgagaacat ccagccaggg caggagtata acttcctgaa gatggagcct caggaggtgg 841 agtccctggg ggagacctat gacttcgaca gcatcatgca ttacgctcgg aacacattct 901 ccaggggcat cttcctggat accattgtcc ccaagtatga ggtgaacggg gtgaaacctc 961 ccattggcca aaggacacgg ctcagcaagg gggacattgc ccaagcccgc aagctttaca 1021 agtgcccagc ctgtggagag accctgcaag acagcacagg caacttctcc tcccctgaat 1081 accccaatgg ctactctgct cacatgcact gcgtgtggcg catctctgtc acacccgggg 1141 agaagatcat cctgaacttc acgtccctgg acctgtaccg cagccgcctg tgctggtacg 1201 actatgtgga ggtccgagat ggcttctgga ggaaggcgcc cctccgaggc cgcttctgcg 1261 ggtccaaact ccctgagcct atcgtctcca ctgacagccg cctctgggtt gaattccgca 1321 gcagcagcaa ttgggttgga aagggcttct ttgcagtcta cgaagccatc tgcgggggtg 1381 atgtgaaaaa ggactatggc cacattcaat cgcccaacta cccagacgat taccggccca 1441 gcaaagtctg catctggcgg atccaggtgt ctgagggctt ccacgtgggc ctcacattcc 1501 agtcctttga gattgagcgc cacgacagct gtgcctacga ctatctggag gtgcgcgacg 1561 ggcacagtga gagcagcacc ctcatcgggc gctactgtgg ctatgagaag cctgatgaca 1621 tcaagagcac gtccagccgc ctctggctca agttcgtctc tgacgggtcc attaacaaag 1681 cgggctttgc cgtcaacttt ttcaaagagg tggacgagtg ctctcggccc aaccgcgggg 1741 gctgtgagca gcggtgcctc aacaccctgg gcagctacaa gtgcagctgt gaccccgggt 1801 acgagctggc cccagacaag cgccgctgtg aggctgcttg tggcggattc ctcaccaagc 1861 tcaacggctc catcaccagc ccgggctggc ccaaggagta cccccccaac aagaactgca 1921 tctggcagct ggtggccccc acccagtacc gcatctccct gcagtttgac ttctttgaga 1981 cagagggcaa tgatgtgtgc aagtacgact tcgtggaggt gcgcagtgga ctcacagctg 2041 actccaagct gcatggcaag ttctgtggtt ctgagaagcc cgaggtcatc acctcccagt 2101 acaacaacat gcgcgtggag ttcaagtccg acaacaccgt gtccaaaaag ggcttcaagg 2161 cccacttctt ctcagacaag gacgagtgct ccaaggataa cggcggctgc cagcaggact 2221 gcatcaacac gttcggcagt tatgagtgcc aatgccgcag tggcttcgtc ctccatgaca 2281 acaagcacga ctgcaaagaa gccggctgtg accacaaggt gacatccacc agtggtacca 2341 tcaccagccc caactggcct gacaagtatc ccagcaagaa tgagtgcacg tgggccatct 2401 ccagcacccc cgggcaccgg gtcaagctga ccttcatgga gatggacatc gagtcccagc 2461 ctgagtgtgc ctacgaccac ctagaggtgt tcgacgggcg agacgccaag gcccccgtcc 2521 tcggccgctt ctgtgggagc aagaagcccg agcccgtcct ggccacaggc agccgcatgt 2581 tcctgcgctt ctactcagat aactcggtcc agcgaaaggg cttccaggcc tcccacgcca 2641 cagagtgcgg gggccaggta cgggcagacg tgaagaccaa ggacctttac tcccacgccc 2701 agtttggcga caacaactac cctgggggtg tggactgtga gtgggtcatt gtggccgagg 2761 aaggctacgg cgtggagctc gtgttccaga cctttgaggt ggaggaggag accgactgcg 2821 gctatgacta catggagctc ttcgacggct acgacagcac agcccccagg ctggggcgct 2881 actgtggctc agggcctcct gaggaggtgt actcggcggg agattctgtc ctggtgaagt 2941 tccactcgga tgacaccatc accaaaaaag gtttccacct gcgatacacc agcaccaagt 3001 tccaggacac actccacagc aggaagttgc caactttctt gtacaaagtg gttggtaagc 3061 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 3121 ggcgcgttaa gtcgacaatc aacctctgga tta