Construct: ORF ccsbBroad304_05899
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012167.1_s304c1, BRDN0000409498
- Derived from:
- ccsbBroadEn_05899
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BMP4 (652)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05899
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 652 | BMP4 | bone morphogenetic protein 4 | NM_001202.6 | 99.9% | 99.7% | 455T>C |
| 2 | human | 652 | BMP4 | bone morphogenetic protein 4 | NM_001347914.1 | 99.9% | 99.7% | 455T>C |
| 3 | human | 652 | BMP4 | bone morphogenetic protein 4 | NM_001347916.1 | 99.9% | 99.7% | 455T>C |
| 4 | human | 652 | BMP4 | bone morphogenetic protein 4 | NM_130850.5 | 99.9% | 99.7% | 455T>C |
| 5 | human | 652 | BMP4 | bone morphogenetic protein 4 | NM_130851.3 | 99.9% | 99.7% | 455T>C |
| 6 | human | 652 | BMP4 | bone morphogenetic protein 4 | NM_001347912.1 | 88.1% | 86.3% | (many diffs) |
| 7 | human | 652 | BMP4 | bone morphogenetic protein 4 | NM_001347913.1 | 84.4% | 84.3% | 0_1ins189;266T>C |
| 8 | human | 652 | BMP4 | bone morphogenetic protein 4 | NM_001347915.1 | 84.4% | 84.3% | 0_1ins189;266T>C |
| 9 | human | 652 | BMP4 | bone morphogenetic protein 4 | NM_001347917.1 | 84.4% | 84.3% | 0_1ins189;266T>C |
| 10 | mouse | 12159 | Bmp4 | bone morphogenetic protein 4 | NM_001316360.1 | 92.1% | 96.8% | (many diffs) |
| 11 | mouse | 12159 | Bmp4 | bone morphogenetic protein 4 | NM_007554.3 | 92.1% | 96.8% | (many diffs) |
| 12 | mouse | 12159 | Bmp4 | bone morphogenetic protein 4 | XM_006518467.3 | 92.1% | 96.8% | (many diffs) |
| 13 | mouse | 12159 | Bmp4 | bone morphogenetic protein 4 | XM_006518468.3 | 92.1% | 96.8% | (many diffs) |
| 14 | mouse | 12159 | Bmp4 | bone morphogenetic protein 4 | XM_011244934.2 | 92.1% | 96.8% | (many diffs) |
| 15 | mouse | 12159 | Bmp4 | bone morphogenetic protein 4 | XM_011244935.2 | 92.1% | 96.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1290
- ORF length:
- 1224
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgat tcctggtaac cgaatgctga tggtcgtttt attatgccaa gtcctgctag 121 gaggcgcgag ccatgctagt ttgatacctg agacggggaa gaaaaaagtc gccgagattc 181 agggccacgc gggaggacgc cgctcagggc agagccatga gctcctgcgg gacttcgagg 241 cgacacttct gcagatgttt gggctgcgcc gccgcccgca gcctagcaag agtgccgtca 301 ttccggacta catgcgggat ctttaccggc ttcagtctgg ggaggaggag gaagagcaga 361 tccacagcac tggtcttgag tatcctgagc gcccggccag ccgggccaac accgtgagga 421 gcttccacca cgaagaacat ctggagaaca tcccagggac cagtgaaaac tctgcttttc 481 gtttcctctt taacctcagc agcatccctg agaacgaggc gatctcctct gcagagcttc 541 ggctcttccg ggagcaggtg gaccagggcc ctgattggga aaggggcttc caccgtataa 601 acatttatga ggttatgaag cccccagcag aagtggtgcc tgggcacctc atcacacgac 661 tactggacac gagactggtc caccacaatg tgacacggtg ggaaactttt gatgtgagcc 721 ctgcggtcct tcgctggacc cgggagaagc agccaaacta tgggctagcc attgaggtga 781 ctcacctcca tcagactcgg acccaccagg gccagcatgt caggattagc cgatcgttac 841 ctcaagggag tgggaattgg gcccagctcc ggcccctcct ggtcaccttt ggccatgatg 901 gccggggcca tgccttgacc cgacgccgga gggccaagcg tagccctaag catcactcac 961 agcgggccag gaagaagaat aagaactgcc ggcgccactc gctctatgtg gacttcagcg 1021 atgtgggctg gaatgactgg attgtggccc caccaggcta ccaggccttc tactgccatg 1081 gggactgccc ctttccactg gctgaccacc tcaactcaac caaccatgcc attgtgcaga 1141 ccctggtcaa ttctgtcaat tccagtatcc ccaaagcctg ttgtgtgccc actgaactga 1201 gtgccatctc catgctgtac ctggatgagt atgataaggt ggtactgaaa aattatcagg 1261 agatggtagt agagggatgt gggtgccgct gcccaacttt cttgtacaaa gtggttggta 1321 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 1381 ggtggcgcgt taagtcgaca atcaacctct ggatta