Construct: ORF ccsbBroad304_06080
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010025.1_s304c1, BRDN0000410502
- Derived from:
- ccsbBroadEn_06080
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DAD1 (1603)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06080
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1603 | DAD1 | defender against cell death 1 | NM_001344.4 | 99.7% | 99.1% | 59C>T |
| 2 | mouse | 13135 | Dad1 | defender against cell death 1 | NM_001113358.1 | 91.1% | 99.1% | (many diffs) |
| 3 | mouse | 13135 | Dad1 | defender against cell death 1 | NM_010015.4 | 91.1% | 99.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 405
- ORF length:
- 339
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggcgtcggta gtgtctgtca tttcgcggtt cttagaagag tacttgagct 121 ccattccgca gcgtctgaag ttgctggacg cgtacctgct gtatatactg ctgaccgggg 181 cgctgcagtt cggttactgt ctcctcgtgg ggaccttccc cttcaactct tttctctcgg 241 gcttcatctc ttgtgtgggg agtttcatcc tagcggtttg cctgagaata cagatcaacc 301 cacagaacaa agcggatttc caaggcatct ccccagagcg agcctttgct gattttctct 361 ttgccagcac catcctgcac cttgttgtca tgaactttgt tggctgccta actttcttgt 421 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 481 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a