Construct: ORF ccsbBroad304_06160

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014953.1_s304c1, BRDN0000399014
Derived from:
ccsbBroadEn_06160
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ENO1 (2023)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06160
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2023 ENO1 enolase 1 NM_001428.5 99.9% 99.7% 1290C>A
2 human 2023 ENO1 enolase 1 NM_001353346.3 97.6% 97.2% (many diffs)
3 human 2023 ENO1 enolase 1 NM_001201483.4 78.4% 78.3% 0_1ins279;1011C>A
4 mouse 433182 Eno1b enolase 1B, retrotransposed NM_001025388.1 88.1% 94.4% (many diffs)
5 mouse 13806 Eno1 enolase 1, alpha non-neuron NM_023119.2 88% 94.4% (many diffs)
6 mouse 13806 Eno1 enolase 1, alpha non-neuron XM_006538525.2 88% 94.4% (many diffs)
7 mouse 13806 Eno1 enolase 1, alpha non-neuron XM_017319953.1 85.8% 92.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1368
ORF length:
1302
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtc tattctcaag atccatgcca gggagatctt tgactctcgc gggaatccca
121ctgttgaggt tgatctcttc acctcaaaag gtctcttcag agctgctgtg cccagtggtg
181cttcaactgg tatctatgag gccctagagc tccgggacaa tgataagact cgctatatgg
241ggaagggtgt ctcaaaggct gttgagcaca tcaataaaac tattgcgcct gccctggtta
301gcaagaaact gaacgtcaca gaacaagaga agattgacaa actgatgatc gagatggatg
361gaacagaaaa taaatctaag tttggtgcga acgccattct gggggtgtcc cttgccgtct
421gcaaagctgg tgccgttgag aagggggtcc ccctgtaccg ccacatcgct gacttggctg
481gcaactctga agtcatcctg ccagtcccgg cgttcaatgt catcaatggc ggttctcatg
541ctggcaacaa gctggccatg caggagttca tgatcctccc agtcggtgca gcaaacttca
601gggaagccat gcgcattgga gcagaggttt accacaacct gaagaatgtc atcaaggaga
661aatatgggaa agatgccacc aatgtggggg atgaaggcgg gtttgctccc aacatcctgg
721agaataaaga aggcctggag ctgctgaaga ctgctattgg gaaagctggc tacactgata
781aggtggtcat cggcatggac gtagcggcct ccgagttctt caggtctggg aagtatgacc
841tggacttcaa gtctcccgat gaccccagca ggtacatctc gcctgaccag ctggctgacc
901tgtacaagtc cttcatcaag gactacccag tggtgtctat cgaagatccc tttgaccagg
961atgactgggg agcttggcag aagttcacag ccagtgcagg aatccaggta gtgggggatg
1021atctcacagt gaccaaccca aagaggatcg ccaaggccgt gaacgagaag tcctgcaact
1081gcctcctgct caaagtcaac cagattggct ccgtgaccga gtctcttcag gcgtgcaagc
1141tggcccaggc caatggttgg ggcgtcatgg tgtctcatcg ttcgggggag actgaagata
1201ccttcatcgc tgacctggtt gtggggctgt gcactgggca gatcaagact ggtgcccctt
1261gccgatctga gcgcttggcc aagtacaacc agctcctcag aattgaagag gagctgggca
1321gcaaggctaa gtttgccggc aggaacttca gaaaaccctt ggccaagtac ccaactttct
1381tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
1441aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

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