Construct: ORF ccsbBroad304_06229
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008738.1_s304c1, BRDN0000408118
- Derived from:
- ccsbBroadEn_06229
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- FUS (2521)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06229
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2521 | FUS | FUS RNA binding protein | NM_004960.3 | 99.9% | 100% | 291C>T |
| 2 | human | 2521 | FUS | FUS RNA binding protein | NM_001170634.1 | 99.7% | 99.6% | 190_191insCAG;288C>T |
| 3 | human | 2521 | FUS | FUS RNA binding protein | XM_011545781.1 | 99.5% | 99.6% | 291C>T;498_499insGGTGGA |
| 4 | human | 2521 | FUS | FUS RNA binding protein | XM_024450221.1 | 99.3% | 99.2% | 190_191insCAG;288C>T;495_496insGGTGGA |
| 5 | human | 2521 | FUS | FUS RNA binding protein | NM_001170937.1 | 99.1% | 99.2% | 291C>T;498_499insGGTGGAGGTGGA |
| 6 | human | 2521 | FUS | FUS RNA binding protein | XM_005255233.5 | 61% | 45.8% | 0_1ins580;183_184ins35 |
| 7 | human | 2521 | FUS | FUS RNA binding protein | XM_011545782.2 | 61% | 45.8% | 0_1ins580;183_184ins35 |
| 8 | human | 2521 | FUS | FUS RNA binding protein | NR_028388.2 | 30.1% | (many diffs) | |
| 9 | mouse | 233908 | Fus | fused in sarcoma | NM_139149.2 | 87.9% | 95% | (many diffs) |
| 10 | mouse | 233908 | Fus | fused in sarcoma | NM_001347649.1 | 87.7% | 94.7% | (many diffs) |
| 11 | mouse | 233908 | Fus | fused in sarcoma | XM_006507760.1 | 54% | 47.8% | (many diffs) |
| 12 | mouse | 233908 | Fus | fused in sarcoma | XM_017322218.1 | 54% | 47.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1644
- ORF length:
- 1578
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 tggacatggc ctcaaacgat tatacccaac aagcaaccca aagctatggg gcctacccca 121 cccagcccgg gcagggctat tcccagcaga gcagtcagcc ctacggacag cagagttaca 181 gtggttatag ccagtccacg gacacttcag gctatggcca gagcagctat tcttcttatg 241 gccagagcca gaacacaggc tatggaactc agtcaactcc ccagggatat ggctcgactg 301 gcggctatgg cagtagccag agctcccaat cgtcttacgg gcagcagtcc tcctatcctg 361 gctatggcca gcagccagct cccagcagca cctcgggaag ttacggtagc agttctcaga 421 gcagcagcta tgggcagccc cagagtggga gctacagcca gcagcctagc tatggtggac 481 agcagcaaag ctatggacag cagcaaagct ataatccccc tcagggctat ggacagcaga 541 accagtacaa cagcagcagt ggtggtggag gtggaggtgg aggtggaggt aactatggcc 601 aagatcaatc ctccatgagt agtggtggtg gcagtggtgg cggttatggc aatcaagacc 661 agagtggtgg aggtggcagc ggtggctatg gacagcagga ccgtggaggc cgcggcaggg 721 gtggcagtgg tggcggcggc ggcggcggcg gtggtggtta caaccgcagc agtggtggct 781 atgaacccag aggtcgtgga ggtggccgtg gaggcagagg tggcatgggc ggaagtgacc 841 gtggtggctt caataaattt ggtggccctc gggaccaagg atcacgtcat gactccgaac 901 aggataattc agacaacaac accatctttg tgcaaggcct gggtgagaat gttacaattg 961 agtctgtggc tgattacttc aagcagattg gtattattaa gacaaacaag aaaacgggac 1021 agcccatgat taatttgtac acagacaggg aaactggcaa gctgaaggga gaggcaacgg 1081 tctcttttga tgacccacct tcagctaaag cagctattga ctggtttgat ggtaaagaat 1141 tctccggaaa tcctatcaag gtctcatttg ctactcgccg ggcagacttt aatcggggtg 1201 gtggcaatgg tcgtggaggc cgagggcgag gaggacccat gggccgtgga ggctatggag 1261 gtggtggcag tggtggtggt ggccgaggag gatttcccag tggaggtggt ggcggtggag 1321 gacagcagcg agctggtgac tggaagtgtc ctaatcccac ctgtgagaat atgaacttct 1381 cttggaggaa tgaatgcaac cagtgtaagg cccctaaacc agatggccca ggagggggac 1441 caggtggctc tcacatgggg ggtaactacg gggatgatcg tcgtggtggc agaggaggct 1501 atgatcgagg cggctaccgg ggccgcggcg gggaccgtgg aggcttccga gggggccggg 1561 gtggtgggga cagaggtggc tttggccctg gcaagatgga ttccaggggt gagcacagac 1621 aggatcgcag ggagaggccg tattaattaa ctttcttgta caaagtggtt ggtaagccta 1681 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 1741 gcgttaagtc gacaatcaac ctctggatta