Construct: ORF ccsbBroad304_06248
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001852.1_s304c1, BRDN0000403794
- Derived from:
- ccsbBroadEn_06248
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GAD1 (2571)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06248
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2571 | GAD1 | glutamate decarboxylase 1 | NM_000817.3 | 99.9% | 99.8% | 872A>G |
| 2 | human | 2571 | GAD1 | glutamate decarboxylase 1 | XM_011510922.1 | 99.9% | 99.8% | 872A>G |
| 3 | human | 2571 | GAD1 | glutamate decarboxylase 1 | XM_017003756.1 | 99.9% | 99.8% | 872A>G |
| 4 | human | 2571 | GAD1 | glutamate decarboxylase 1 | XM_017003757.2 | 66.4% | 66.3% | 872A>G;1185_1186ins597 |
| 5 | human | 2571 | GAD1 | glutamate decarboxylase 1 | XM_024452783.1 | 56.8% | 56.7% | 0_1ins768;104A>G |
| 6 | human | 2571 | GAD1 | glutamate decarboxylase 1 | NM_013445.3 | 37.2% | 36% | (many diffs) |
| 7 | human | 2571 | GAD1 | glutamate decarboxylase 1 | XM_005246444.3 | 37.2% | 36% | (many diffs) |
| 8 | human | 2571 | GAD1 | glutamate decarboxylase 1 | XM_017003758.2 | 37.2% | 36% | (many diffs) |
| 9 | mouse | 14415 | Gad1 | glutamate decarboxylase 1 | NM_008077.5 | 91.3% | 96.9% | (many diffs) |
| 10 | mouse | 14415 | Gad1 | glutamate decarboxylase 1 | XM_006498765.3 | 91.3% | 96.9% | (many diffs) |
| 11 | mouse | 14415 | Gad1 | glutamate decarboxylase 1 | XM_006498766.3 | 91.3% | 96.9% | (many diffs) |
| 12 | mouse | 14415 | Gad1 | glutamate decarboxylase 1 | XM_017315594.1 | 87.2% | 92.7% | (many diffs) |
| 13 | mouse | 14415 | Gad1 | glutamate decarboxylase 1 | XM_017315597.1 | 87.2% | 92.7% | (many diffs) |
| 14 | mouse | 14415 | Gad1 | glutamate decarboxylase 1 | XM_017315599.1 | 87.2% | 92.7% | (many diffs) |
| 15 | mouse | 14415 | Gad1 | glutamate decarboxylase 1 | XM_006498767.2 | 57.3% | 60.4% | (many diffs) |
| 16 | mouse | 14415 | Gad1 | glutamate decarboxylase 1 | XR_001780815.1 | 43.7% | (many diffs) | |
| 17 | mouse | 14415 | Gad1 | glutamate decarboxylase 1 | NM_001312900.1 | 33.6% | 34.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1848
- ORF length:
- 1782
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gtcttcgacc ccatcttcgt ccgcaacctc ctcgaacgcg ggagcggacc 121 ccaataccac taacctgcgc cccacaacgt acgatacctg gtgcggcgtg gcccatggat 181 gcaccagaaa actggggctc aagatctgcg gcttcttgca aaggaccaac agcctggaag 241 agaagagtcg ccttgtgagt gccttcaagg agaggcaatc ctccaagaac ctgctttcct 301 gtgaaaacag cgaccgggat gcccgcttcc ggcgcacaga gactgacttc tctaatctgt 361 ttgctagaga tctgcttccg gctaagaacg gtgaggagca aaccgtgcaa ttcctcctgg 421 aagtggtgga catactcctc aactatgtcc gcaagacatt tgatcgctcc accaaggtgc 481 tggactttca tcacccacac cagttgctgg aaggcatgga gggcttcaac ttggagctct 541 ctgaccaccc cgagtccctg gagcagatcc tggttgactg cagagacacc ttgaagtatg 601 gggttcgcac aggtcatcct cgatttttca accagctctc cactggattg gatattattg 661 gcctagctgg agaatggctg acatcaacgg ccaataccaa catgtttaca tatgaaattg 721 caccagtgtt tgtcctcatg gaacaaataa cacttaagaa gatgagagag atagttggat 781 ggtcaagtaa agatggtgat gggatatttt ctcctggggg cgccatatcc aacatgtaca 841 gcatcatggc tgctcgctac aagtacttcc cggaagttaa gacaaagggc atggcggctg 901 tgcctaaact ggtcctcttc acctcagaac agagtcgcta ttccataaag aaagctgggg 961 ctgcacttgg ctttggaact gacaatgtga ttttgataaa gtgcaatgaa agggggaaaa 1021 taattccagc tgattttgag gcaaaaattc ttgaagccaa acagaaggga tatgttccct 1081 tttatgtcaa tgcaactgct ggcacgactg tttatggagc ttttgatccg atacaagaga 1141 ttgcagatat atgtgagaaa tataaccttt ggttgcatgt cgatgctgcc tggggaggtg 1201 ggctgctcat gtccaggaag caccgccata aactcaacgg catagaaagg gccaactcag 1261 tcacctggaa ccctcacaag atgatgggcg tgctgttgca gtgctctgcc attctcgtca 1321 aggaaaaggg tatactccaa ggatgcaacc agatgtgtgc aggatacctc ttccagccag 1381 acaagcagta tgatgtctcc tacgacaccg gggacaaggc aattcagtgt ggccgccacg 1441 tggatatctt caagttctgg ctgatgtgga aagcaaaggg cacagtggga tttgaaaacc 1501 agatcaacaa atgcctggaa ctggctgaat acctctatgc caagattaaa aacagagaag 1561 aatttgagat ggttttcaat ggcgagcctg agcacacaaa cgtctgtttt tggtatattc 1621 cacaaagcct caggggtgtg ccagacagcc ctcaacgacg ggaaaagcta cacaaggtgg 1681 ctccaaaaat caaagccctg atgatggagt caggtacgac catggttggc taccagcccc 1741 aaggggacaa ggccaacttc ttccggatgg tcatctccaa cccagccgct acccagtctg 1801 acattgactt cctcattgag gagatagaaa gactgggcca ggatctgtac ccaactttct 1861 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 1921 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta