Construct: ORF ccsbBroad304_06248

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001852.1_s304c1, BRDN0000403794
Derived from:
ccsbBroadEn_06248
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GAD1 (2571)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06248
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2571 GAD1 glutamate decarboxylase 1 NM_000817.3 99.9% 99.8% 872A>G
2 human 2571 GAD1 glutamate decarboxylase 1 XM_011510922.1 99.9% 99.8% 872A>G
3 human 2571 GAD1 glutamate decarboxylase 1 XM_017003756.1 99.9% 99.8% 872A>G
4 human 2571 GAD1 glutamate decarboxylase 1 XM_017003757.2 66.4% 66.3% 872A>G;1185_1186ins597
5 human 2571 GAD1 glutamate decarboxylase 1 XM_024452783.1 56.8% 56.7% 0_1ins768;104A>G
6 human 2571 GAD1 glutamate decarboxylase 1 NM_013445.3 37.2% 36% (many diffs)
7 human 2571 GAD1 glutamate decarboxylase 1 XM_005246444.3 37.2% 36% (many diffs)
8 human 2571 GAD1 glutamate decarboxylase 1 XM_017003758.2 37.2% 36% (many diffs)
9 mouse 14415 Gad1 glutamate decarboxylase 1 NM_008077.5 91.3% 96.9% (many diffs)
10 mouse 14415 Gad1 glutamate decarboxylase 1 XM_006498765.3 91.3% 96.9% (many diffs)
11 mouse 14415 Gad1 glutamate decarboxylase 1 XM_006498766.3 91.3% 96.9% (many diffs)
12 mouse 14415 Gad1 glutamate decarboxylase 1 XM_017315594.1 87.2% 92.7% (many diffs)
13 mouse 14415 Gad1 glutamate decarboxylase 1 XM_017315597.1 87.2% 92.7% (many diffs)
14 mouse 14415 Gad1 glutamate decarboxylase 1 XM_017315599.1 87.2% 92.7% (many diffs)
15 mouse 14415 Gad1 glutamate decarboxylase 1 XM_006498767.2 57.3% 60.4% (many diffs)
16 mouse 14415 Gad1 glutamate decarboxylase 1 XR_001780815.1 43.7% (many diffs)
17 mouse 14415 Gad1 glutamate decarboxylase 1 NM_001312900.1 33.6% 34.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1848
ORF length:
1782
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc gtcttcgacc ccatcttcgt ccgcaacctc ctcgaacgcg ggagcggacc
121ccaataccac taacctgcgc cccacaacgt acgatacctg gtgcggcgtg gcccatggat
181gcaccagaaa actggggctc aagatctgcg gcttcttgca aaggaccaac agcctggaag
241agaagagtcg ccttgtgagt gccttcaagg agaggcaatc ctccaagaac ctgctttcct
301gtgaaaacag cgaccgggat gcccgcttcc ggcgcacaga gactgacttc tctaatctgt
361ttgctagaga tctgcttccg gctaagaacg gtgaggagca aaccgtgcaa ttcctcctgg
421aagtggtgga catactcctc aactatgtcc gcaagacatt tgatcgctcc accaaggtgc
481tggactttca tcacccacac cagttgctgg aaggcatgga gggcttcaac ttggagctct
541ctgaccaccc cgagtccctg gagcagatcc tggttgactg cagagacacc ttgaagtatg
601gggttcgcac aggtcatcct cgatttttca accagctctc cactggattg gatattattg
661gcctagctgg agaatggctg acatcaacgg ccaataccaa catgtttaca tatgaaattg
721caccagtgtt tgtcctcatg gaacaaataa cacttaagaa gatgagagag atagttggat
781ggtcaagtaa agatggtgat gggatatttt ctcctggggg cgccatatcc aacatgtaca
841gcatcatggc tgctcgctac aagtacttcc cggaagttaa gacaaagggc atggcggctg
901tgcctaaact ggtcctcttc acctcagaac agagtcgcta ttccataaag aaagctgggg
961ctgcacttgg ctttggaact gacaatgtga ttttgataaa gtgcaatgaa agggggaaaa
1021taattccagc tgattttgag gcaaaaattc ttgaagccaa acagaaggga tatgttccct
1081tttatgtcaa tgcaactgct ggcacgactg tttatggagc ttttgatccg atacaagaga
1141ttgcagatat atgtgagaaa tataaccttt ggttgcatgt cgatgctgcc tggggaggtg
1201ggctgctcat gtccaggaag caccgccata aactcaacgg catagaaagg gccaactcag
1261tcacctggaa ccctcacaag atgatgggcg tgctgttgca gtgctctgcc attctcgtca
1321aggaaaaggg tatactccaa ggatgcaacc agatgtgtgc aggatacctc ttccagccag
1381acaagcagta tgatgtctcc tacgacaccg gggacaaggc aattcagtgt ggccgccacg
1441tggatatctt caagttctgg ctgatgtgga aagcaaaggg cacagtggga tttgaaaacc
1501agatcaacaa atgcctggaa ctggctgaat acctctatgc caagattaaa aacagagaag
1561aatttgagat ggttttcaat ggcgagcctg agcacacaaa cgtctgtttt tggtatattc
1621cacaaagcct caggggtgtg ccagacagcc ctcaacgacg ggaaaagcta cacaaggtgg
1681ctccaaaaat caaagccctg atgatggagt caggtacgac catggttggc taccagcccc
1741aaggggacaa ggccaacttc ttccggatgg tcatctccaa cccagccgct acccagtctg
1801acattgactt cctcattgag gagatagaaa gactgggcca ggatctgtac ccaactttct
1861tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
1921aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

Download FASTA (ORF) (Full)