Construct: ORF ccsbBroad304_06327

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012039.1_s304c1, BRDN0000462212
Derived from:
ccsbBroadEn_06327
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GRM8 (2918)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06327
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2918 GRM8 glutamate metabotropic rece... NM_000845.3 99.9% 99.7% 17A>G;2486C>T
2 human 2918 GRM8 glutamate metabotropic rece... NM_001371083.1 99.9% 99.7% 17A>G;2486C>T
3 human 2918 GRM8 glutamate metabotropic rece... NM_001371084.1 99.9% 99.7% 17A>G;2486C>T
4 human 2918 GRM8 glutamate metabotropic rece... NM_001127323.1 98.5% 98.2% (many diffs)
5 human 2918 GRM8 glutamate metabotropic rece... NM_001371085.1 98.5% 98.2% (many diffs)
6 human 2918 GRM8 glutamate metabotropic rece... NM_001371086.1 97.7% 96.2% (many diffs)
7 human 2918 GRM8 glutamate metabotropic rece... XM_011516094.1 80.5% 79.7% (many diffs)
8 human 2918 GRM8 glutamate metabotropic rece... NM_001371087.1 77.3% 77.3% 0_1ins615;1871C>T
9 human 2918 GRM8 glutamate metabotropic rece... XM_017012075.1 77.3% 77.3% 0_1ins615;1871C>T
10 human 2918 GRM8 glutamate metabotropic rece... XM_017012076.1 77.3% 77.3% 0_1ins615;1871C>T
11 human 2918 GRM8 glutamate metabotropic rece... XM_017012077.1 77.3% 77.3% 0_1ins615;1871C>T
12 human 2918 GRM8 glutamate metabotropic rece... XM_017012078.1 77.3% 77.3% 0_1ins615;1871C>T
13 human 2918 GRM8 glutamate metabotropic rece... XM_017012079.1 77.3% 77.3% 0_1ins615;1871C>T
14 human 2918 GRM8 glutamate metabotropic rece... XM_011516095.2 77.2% 76.8% (many diffs)
15 human 2918 GRM8 glutamate metabotropic rece... XM_011516101.2 76% 75.7% (many diffs)
16 human 2918 GRM8 glutamate metabotropic rece... XM_011516102.2 76% 75.7% (many diffs)
17 human 2918 GRM8 glutamate metabotropic rece... NR_028041.1 74.7% (many diffs)
18 human 2918 GRM8 glutamate metabotropic rece... NR_163850.1 69.7% (many diffs)
19 human 2918 GRM8 glutamate metabotropic rece... NR_163849.1 68.4% (many diffs)
20 human 2918 GRM8 glutamate metabotropic rece... XM_011516103.2 67.9% 67.6% (many diffs)
21 human 2918 GRM8 glutamate metabotropic rece... XM_017012080.1 67.9% 67.6% (many diffs)
22 human 2918 GRM8 glutamate metabotropic rece... XM_024446736.1 67.9% 67.6% (many diffs)
23 human 2918 GRM8 glutamate metabotropic rece... XM_011516108.2 58.6% 56.5% (many diffs)
24 human 2918 GRM8 glutamate metabotropic rece... NM_001371088.1 50% 49.6% (many diffs)
25 mouse 14823 Grm8 glutamate receptor, metabot... NM_001311072.1 91.8% 98.2% (many diffs)
26 mouse 14823 Grm8 glutamate receptor, metabot... XM_017321397.1 91.8% 98.2% (many diffs)
27 mouse 14823 Grm8 glutamate receptor, metabot... NM_008174.2 90.8% 96.6% (many diffs)
28 mouse 14823 Grm8 glutamate receptor, metabot... XM_006504987.3 90.8% 96.6% (many diffs)
29 mouse 14823 Grm8 glutamate receptor, metabot... XM_006504989.1 90.8% 96.6% (many diffs)
30 mouse 14823 Grm8 glutamate receptor, metabot... XM_006504990.3 90.8% 96.6% (many diffs)
31 mouse 14823 Grm8 glutamate receptor, metabot... XM_006504991.3 90.8% 96.6% (many diffs)
32 mouse 14823 Grm8 glutamate receptor, metabot... XM_017321398.1 62.8% 66.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2793
ORF length:
2724
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggtatgcgag ggaaggcgat cagcctcttg cccttgtttc ttcctcttga
121ccgccaagtt ctactggatc ctcacaatga tgcaaagaac tcacagccag gagtatgccc
181attccatacg ggtggatggg gacattattt tggggggtct cttccctgtc cacgcaaagg
241gagagagagg ggtgccttgt ggggagctga agaaggaaaa ggggattcac agactggagg
301ccatgcttta tgcaattgac cagattaaca aggaccctga tctcctttcc aacatcactc
361tgggtgtccg catcctcgac acgtgctcta gggacaccta tgctttggag cagtctctaa
421cattcgtgca ggcattaata gagaaagatg cttcggatgt gaagtgtgct aatggagatc
481cacccatttt caccaagccc gacaagattt ctggcgtcat aggtgctgca gcaagctccg
541tgtccatcat ggttgctaac attttaagac tttttaagat acctcaaatc agctatgcat
601ccacagcccc agagctaagt gataacacca ggtatgactt tttctctcga gtggttccgc
661ctgactccta ccaagcccaa gccatggtgg acatcgtgac agcactggga tggaattatg
721tttcgacact ggcttctgag gggaactatg gtgagagcgg tgtggaggcc ttcacccaga
781tctcgaggga gattggtggt gtttgcattg ctcagtcaca gaaaatccca cgtgaaccaa
841gacctggaga atttgaaaaa attatcaaac gcctgctaga aacacctaat gctcgagcag
901tgattatgtt tgccaatgag gatgacatca ggaggatatt ggaagcagca aaaaaactaa
961accaaagtgg gcattttctc tggattggct cagatagttg gggatccaaa atagcacctg
1021tctatcagca agaggagatt gcagaagggg ctgtgacaat tttgcccaaa cgagcatcaa
1081ttgatggatt tgatcgatac tttagaagcc gaactcttgc caataatcga agaaatgtgt
1141ggtttgcaga attctgggag gagaattttg gctgcaagtt aggatcacat gggaaaagga
1201acagtcatat aaagaaatgc acagggctgg agcgaattgc tcgggattca tcttatgaac
1261aggaaggaaa ggtccaattt gtaattgatg ctgtatattc catggcttac gccctgcaca
1321atatgcacaa agatctctgc cctggataca ttggcctttg tccacgaatg agtaccattg
1381atgggaaaga gctacttggt tatattcggg ctgtaaattt taatggcagt gctggcactc
1441ctgtcacttt taatgaaaac ggagatgctc ctggacgtta tgatatcttc cagtatcaaa
1501taaccaacaa aagcacagag tacaaagtca tcggccactg gaccaatcag cttcatctaa
1561aagtggaaga catgcagtgg gctcatagag aacatactca cccggcgtct gtctgcagcc
1621tgccgtgtaa gccaggggag aggaagaaaa cggtgaaagg ggtcccttgc tgctggcact
1681gtgaacgctg tgaaggttac aactaccagg tggatgagct gtcctgtgaa ctttgccctc
1741tggatcagag acccaacatg aaccgcacag gctgccagct tatccccatc atcaaattgg
1801agtggcattc tccctgggct gtggtgcctg tgtttgttgc aatattggga atcatcgcca
1861ccacctttgt gatcgtgacc tttgtccgct ataatgacac acctatcgtg agggcttcag
1921gacgcgaact tagttacgtg ctcctaacgg ggatttttct ctgttattca atcacgtttt
1981taatgattgc agcaccagat acaatcatat gctccttccg acgggtcttc ctaggacttg
2041gcatgtgttt cagctatgca gcccttctga ccaaaacaaa ccgtatccac cgaatatttg
2101agcaggggaa gaaatctgtc acagcgccca agttcattag tccagcatct cagctggtga
2161tcaccttcag cctcatctcc gtccagctcc ttggagtgtt tgtctggttt gttgtggatc
2221ccccccacat catcattgac tatggagagc agcggacact agatccagag aaggccaggg
2281gagtgctcaa gtgtgacatt tctgatctct cactcatttg ttcacttgga tacagtatcc
2341tcttgatggt cacttgtact gtttatgcca ttaaaacgag aggtgtccca gagactttca
2401atgaagccaa acctattgga tttaccatgt ataccacctg catcatttgg ttagctttca
2461tccccatctt ttttggtaca gcccagtcag cagaaaagat gtacatccag acaacaacac
2521ttactgtctc catgagttta agtgcttcag tatttctggg catgctctat atgcccaagg
2581tttatattat aatttttcat ccagaacaga atgttcaaaa acgcaagagg agcttcaagg
2641ctgtggtgac agctgccacc atgcaaagca aactgatcca aaaaggaaat gacagaccaa
2701atggcgaggt gaaaagtgaa ctctgtgaga gtcttgaaac caacacttcc tctaccaaga
2761caacatatat cagttacagc aatcattcaa tcttgccaac tttcttgtac aaagtggttg
2821gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
2881accggtggcg cgttaagtcg acaatcaacc tctggatta

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.