Transcript: Human XM_011516094.1

PREDICTED: Homo sapiens glutamate metabotropic receptor 8 (GRM8), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
GRM8 (2918)
Length:
2805
CDS:
39..2282

Additional Resources:

NCBI RefSeq record:
XM_011516094.1
NBCI Gene record:
GRM8 (2918)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011516094.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000363196 GTGACCTTTGTCCGCTATAAT pLKO_005 1362 CDS 100% 15.000 21.000 N GRM8 n/a
2 TRCN0000219404 CAAACCGTATCCACCGAATAT pLKO.1 1564 CDS 100% 13.200 18.480 N Grm8 n/a
3 TRCN0000363183 CAAACCGTATCCACCGAATAT pLKO_005 1564 CDS 100% 13.200 18.480 N GRM8 n/a
4 TRCN0000219406 CTTGCCAATAATCGAAGAAAT pLKO.1 603 CDS 100% 13.200 9.240 N Grm8 n/a
5 TRCN0000219409 CTTGTACTGTTTATGCCATTA pLKO.1 1840 CDS 100% 10.800 7.560 N Grm8 n/a
6 TRCN0000009038 CCCACATTGTTTAACTTGTAT pLKO.1 2698 3UTR 100% 5.625 3.938 N GRM8 n/a
7 TRCN0000009039 CCGCTATAATGACACACCTAT pLKO.1 1373 CDS 100% 4.950 3.465 N GRM8 n/a
8 TRCN0000009041 CCTGCATCATTTGGTTAGCTT pLKO.1 1924 CDS 100% 3.000 2.100 N GRM8 n/a
9 TRCN0000009042 GCTAAGTGATAACACCAGGTA pLKO.1 101 CDS 100% 2.640 1.848 N GRM8 n/a
10 TRCN0000219405 GATGACATCAGGAGGATATTG pLKO.1 408 CDS 100% 13.200 7.920 N Grm8 n/a
11 TRCN0000363156 GATGACATCAGGAGGATATTG pLKO_005 408 CDS 100% 13.200 7.920 N GRM8 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011516094.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489666 CTGTACCGCGCATAGGTGACCTTG pLX_317 13% 81.8% 81.2% V5 (not translated due to prior stop codon) (many diffs) n/a
2 TRCN0000487954 AGCGCTCGGAAATCGTTCTGTCGG pLX_317 12.3% 81.8% 81.1% V5 (many diffs) n/a
3 TRCN0000492137 GATTACATGGCCTACAAACTATCG pLX_317 12.5% 80.5% 79.8% V5 (not translated due to prior stop codon) (many diffs) n/a
4 ccsbBroadEn_06327 pDONR223 100% 80.5% 79.7% None (many diffs) n/a
5 ccsbBroad304_06327 pLX_304 0% 80.5% 79.7% V5 (many diffs) n/a
6 TRCN0000477307 TTAGTATTTATGGTTTTCTTGATC pLX_317 16.8% 80.5% 79.7% V5 (many diffs) n/a
7 TRCN0000489225 CGCAGCTCTTTACGAAAGTTGGCA pLX_317 12.4% 80.5% 79.7% V5 (many diffs) n/a
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