Construct: ORF ccsbBroad304_06348
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013757.1_s304c1, BRDN0000401710
- Derived from:
- ccsbBroadEn_06348
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H2BC5 (3017)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06348
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3017 | H2BC5 | H2B clustered histone 5 | NM_021063.3 | 99.2% | 99.2% | 13A>G;15C>T;21T>C |
| 2 | human | 3017 | H2BC5 | H2B clustered histone 5 | NM_138720.2 | 99.2% | 99.2% | 13A>G;15C>T;21T>C |
| 3 | human | 8341 | H2BC15 | H2B clustered histone 15 | NM_003520.3 | 93.3% | 99.2% | (many diffs) |
| 4 | human | 8340 | H2BC13 | H2B clustered histone 13 | NM_003519.3 | 93.3% | 98.4% | (many diffs) |
| 5 | human | 8343 | H2BC7 | H2B clustered histone 7 | NM_003522.3 | 93.1% | 100% | (many diffs) |
| 6 | human | 8344 | H2BC6 | H2B clustered histone 6 | NM_003523.2 | 92.8% | 100% | (many diffs) |
| 7 | human | 8346 | H2BC10 | H2B clustered histone 10 | NM_003525.2 | 92.8% | 100% | (many diffs) |
| 8 | human | 8347 | H2BC4 | H2B clustered histone 4 | NM_003526.2 | 92.3% | 100% | (many diffs) |
| 9 | human | 85236 | H2BC12 | H2B clustered histone 12 | NM_001312653.1 | 91.7% | 99.2% | (many diffs) |
| 10 | human | 85236 | H2BC12 | H2B clustered histone 12 | NM_080593.2 | 91.7% | 99.2% | (many diffs) |
| 11 | human | 8345 | H2BC9 | H2B clustered histone 9 | NM_003524.2 | 91.2% | 99.2% | (many diffs) |
| 12 | human | 54145 | H2BS1 | H2B.S histone 1 | NM_017445.3 | 91.2% | 97.6% | (many diffs) |
| 13 | human | 102724334 | LOC102724334 | histone H2B type F-S-like | XM_006723918.4 | 91.2% | 97.6% | (many diffs) |
| 14 | human | 8970 | H2BC11 | H2B clustered histone 11 | NM_021058.3 | 90.7% | 98.4% | (many diffs) |
| 15 | human | 8348 | H2BC17 | H2B clustered histone 17 | NM_003527.4 | 90.4% | 98.4% | (many diffs) |
| 16 | human | 8349 | H2BC21 | H2B clustered histone 21 | NM_003528.2 | 89.9% | 99.2% | (many diffs) |
| 17 | human | 440689 | H2BC18 | H2B clustered histone 18 | NM_001024599.4 | 89.6% | 98.4% | (many diffs) |
| 18 | human | 8339 | H2BC8 | H2B clustered histone 8 | NM_003518.3 | 89.1% | 100% | (many diffs) |
| 19 | human | 8342 | H2BC14 | H2B clustered histone 14 | NM_003521.2 | 89.1% | 96.8% | (many diffs) |
| 20 | human | 3018 | H2BC3 | H2B clustered histone 3 | NM_021062.2 | 88.8% | 97.6% | (many diffs) |
| 21 | human | 128312 | H2BU1 | H2B.U histone 1 | NM_175055.2 | 87.3% | 95.2% | (many diffs) |
| 22 | human | 440689 | H2BC18 | H2B clustered histone 18 | NM_001161334.1 | 84.3% | 92.5% | (many diffs) |
| 23 | human | 3017 | H2BC5 | H2B clustered histone 5 | XM_005249039.4 | 83.3% | 83.3% | (many diffs) |
| 24 | human | 337875 | H2BP1 | H2B histone pseudogene 1 | NR_160944.2 | 67.5% | (many diffs) | |
| 25 | human | 337873 | H2BC20P | H2B clustered histone 20, p... | NR_036461.1 | 57.2% | (many diffs) | |
| 26 | human | 338391 | H2BP2 | H2B histone pseudogene 2 | NR_160915.1 | 21% | (many diffs) | |
| 27 | human | 337875 | H2BP1 | H2B histone pseudogene 1 | NR_027337.2 | 18.3% | (many diffs) | |
| 28 | human | 106479023 | H3P4 | H3 histone pseudogene 4 | NR_160941.1 | 16.9% | (many diffs) | |
| 29 | human | 337875 | H2BP1 | H2B histone pseudogene 1 | NR_160942.1 | 15.7% | (many diffs) | |
| 30 | human | 106479023 | H3P4 | H3 histone pseudogene 4 | NR_160940.1 | 15.1% | (many diffs) | |
| 31 | human | 338391 | H2BP2 | H2B histone pseudogene 2 | NR_160913.1 | 13.7% | (many diffs) | |
| 32 | human | 338391 | H2BP2 | H2B histone pseudogene 2 | NR_160914.1 | 11.2% | (many diffs) | |
| 33 | mouse | 68024 | Hist1h2bc | histone cluster 1, H2bc | NM_001290380.1 | 92.5% | 100% | (many diffs) |
| 34 | mouse | 68024 | Hist1h2bc | histone cluster 1, H2bc | NM_023422.3 | 92.5% | 100% | (many diffs) |
| 35 | mouse | 319180 | Hist1h2bf | histone cluster 1, H2bf | NM_178195.2 | 92.5% | 99.2% | (many diffs) |
| 36 | mouse | 319181 | Hist1h2bg | histone cluster 1, H2bg | NM_178196.4 | 92.3% | 100% | (many diffs) |
| 37 | mouse | 665622 | Hist1h2br | histone cluster 1 H2br | NM_001313878.1 | 92% | 99.2% | (many diffs) |
| 38 | mouse | 665596 | Hist1h2bq | histone cluster 1, H2bq | NM_001313880.1 | 92% | 99.2% | (many diffs) |
| 39 | mouse | 319182 | Hist1h2bh | histone cluster 1, H2bh | NM_178197.2 | 92% | 99.2% | (many diffs) |
| 40 | mouse | 319187 | Hist1h2bn | histone cluster 1, H2bn | NM_178201.2 | 92% | 99.2% | (many diffs) |
| 41 | mouse | 319179 | Hist1h2be | histone cluster 1, H2be | NM_001177653.1 | 91.7% | 100% | (many diffs) |
| 42 | mouse | 319179 | Hist1h2be | histone cluster 1, H2be | NM_001290530.1 | 91.7% | 100% | (many diffs) |
| 43 | mouse | 319179 | Hist1h2be | histone cluster 1, H2be | NM_178194.4 | 91.7% | 100% | (many diffs) |
| 44 | mouse | 319185 | Hist1h2bl | histone cluster 1, H2bl | NM_178199.2 | 91.7% | 99.2% | (many diffs) |
| 45 | mouse | 319183 | Hist1h2bj | histone cluster 1, H2bj | NM_178198.2 | 91.5% | 99.2% | (many diffs) |
| 46 | mouse | 319186 | Hist1h2bm | histone cluster 1, H2bm | NM_178200.2 | 91.5% | 99.2% | (many diffs) |
| 47 | mouse | 319184 | Hist1h2bk | histone cluster 1, H2bk | NM_175665.2 | 91.2% | 98.4% | (many diffs) |
| 48 | mouse | 319188 | Hist1h2bp | histone cluster 1, H2bp | NM_001290466.1 | 91% | 97.6% | (many diffs) |
| 49 | mouse | 319188 | Hist1h2bp | histone cluster 1, H2bp | XM_006516690.2 | 91% | 97.6% | (many diffs) |
| 50 | mouse | 319178 | Hist1h2bb | histone cluster 1, H2bb | NM_175664.3 | 90.2% | 96.8% | (many diffs) |
| 51 | mouse | 319189 | Hist2h2bb | histone cluster 2, H2bb | NM_175666.2 | 88% | 98.4% | (many diffs) |
| 52 | mouse | 665596 | Hist1h2bq | histone cluster 1, H2bq | NM_001097979.2 | 86.3% | 92.5% | (many diffs) |
| 53 | mouse | 665622 | Hist1h2br | histone cluster 1 H2br | NM_001110555.1 | 86.3% | 92.5% | (many diffs) |
| 54 | mouse | 78303 | Hist3h2ba | histone cluster 3, H2ba | NM_030082.4 | 85.8% | 93.6% | (many diffs) |
| 55 | mouse | 319190 | Hist2h2be | histone cluster 2, H2be | NM_178214.4 | 85.7% | 96% | (many diffs) |
| 56 | mouse | 319177 | Hist1h2ba | histone cluster 1, H2ba | NM_175663.2 | 83% | 87.4% | (many diffs) |
| 57 | mouse | 319188 | Hist1h2bp | histone cluster 1, H2bp | NM_178202.2 | 82.8% | 89.1% | (many diffs) |
| 58 | mouse | 382522 | Hist3h2bb-ps | histone cluster 3, H2bb, ps... | NR_134309.1 | 70.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 447
- ORF length:
- 378
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcctgaacct gctaagtccg ctcctgcccc aaagaagggc tccaagaagg 121 cggtgactaa ggctcagaag aaggacggga agaagcgcaa gcgcagccgc aaggagagct 181 attcagtgta tgtgtacaag gtgctgaagc aggtccatcc cgacaccggc atctcttcca 241 aggcaatggg gatcatgaat tccttcgtca acgacatctt cgagcgcatc gcaggcgagg 301 cttcccgcct ggcgcattac aacaagcgct cgaccatcac ctccagggag atccagacgg 361 ccgtgcgcct gctgcttccg ggggagctgg ccaagcacgc cgtgtcggag ggcaccaagg 421 ccgtcaccaa gtacaccagt tccaagttgc caactttctt gtacaaagtg gttggtaagc 481 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 541 ggcgcgttaa gtcgacaatc aacctctgga tta