Construct: ORF ccsbBroad304_06380
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003427.1_s304c1, BRDN0000407723
- Derived from:
- ccsbBroadEn_06380
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HLCS (3141)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06380
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3141 | HLCS | holocarboxylase synthetase | NM_000411.8 | 99.9% | 100% | 834C>T |
| 2 | human | 3141 | HLCS | holocarboxylase synthetase | NM_001242784.3 | 99.9% | 100% | 834C>T |
| 3 | human | 3141 | HLCS | holocarboxylase synthetase | NM_001242785.2 | 99.9% | 100% | 834C>T |
| 4 | human | 3141 | HLCS | holocarboxylase synthetase | NM_001352515.2 | 99.9% | 100% | 834C>T |
| 5 | human | 3141 | HLCS | holocarboxylase synthetase | NM_001352516.2 | 99.9% | 100% | 834C>T |
| 6 | human | 3141 | HLCS | holocarboxylase synthetase | NM_001352517.1 | 99.9% | 100% | 834C>T |
| 7 | human | 3141 | HLCS | holocarboxylase synthetase | NM_001352518.1 | 99.9% | 100% | 834C>T |
| 8 | human | 3141 | HLCS | holocarboxylase synthetase | XM_006723995.1 | 99.9% | 100% | 834C>T |
| 9 | human | 3141 | HLCS | holocarboxylase synthetase | XM_011529538.1 | 99.9% | 100% | 834C>T |
| 10 | human | 3141 | HLCS | holocarboxylase synthetase | XM_011529539.3 | 99.9% | 100% | 834C>T |
| 11 | human | 3141 | HLCS | holocarboxylase synthetase | XM_017028330.1 | 99.9% | 100% | 834C>T |
| 12 | human | 3141 | HLCS | holocarboxylase synthetase | XM_024452065.1 | 92.1% | 92.1% | 0_1ins171;663C>T |
| 13 | human | 3141 | HLCS | holocarboxylase synthetase | XM_024452066.1 | 92.1% | 92.1% | 0_1ins171;663C>T |
| 14 | human | 3141 | HLCS | holocarboxylase synthetase | NM_001352514.2 | 83.1% | 83.1% | 1_441del;1275C>T |
| 15 | human | 3141 | HLCS | holocarboxylase synthetase | XR_001754840.1 | 78.1% | (many diffs) | |
| 16 | human | 3141 | HLCS | holocarboxylase synthetase | XR_001754837.2 | 66.1% | (many diffs) | |
| 17 | human | 3141 | HLCS | holocarboxylase synthetase | XM_011529540.2 | 55.7% | 55.4% | (many diffs) |
| 18 | human | 3141 | HLCS | holocarboxylase synthetase | XR_001754835.1 | 43.1% | (many diffs) | |
| 19 | human | 3141 | HLCS | holocarboxylase synthetase | XR_001754836.1 | 41.8% | (many diffs) | |
| 20 | human | 3141 | HLCS | holocarboxylase synthetase | NR_148020.2 | 26.1% | (many diffs) | |
| 21 | human | 3141 | HLCS | holocarboxylase synthetase | NR_148021.1 | 25.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2244
- ORF length:
- 2178
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga agatagactc cacatggata atggactggt accccaaaag attgtgtcgg 121 tgcacttgca ggactccact ctgaaggaag ttaaggatca ggtctcaaac aagcaagccc 181 agatcctaga gccgaagcct gaaccttctc ttgagattaa gcctgagcag gacggtatgg 241 agcatgttgg cagagatgac ccaaaggctc ttggtgaaga acccaaacaa aggagaggca 301 gtgcctctgg gagtgagcct gctggggaca gtgacagggg agggggcccc gttgagcatt 361 atcacctcca tctgtctagt tgccacgagt gtctggaact tgagaacagc accattgagt 421 cagtcaagtt tgcgtctgcc gagaacattc cagaccttcc ctacgattat agcagcagtt 481 tggagagtgt tgctgatgag acctcccccg aaagagaagg gaggagagtc aacctcacgg 541 gaaaggcacc caacatcctc ctctatgtgg gctccgactc ccaggaagcc ctcggccggt 601 tccacgaggt ccggtctgtg ctggccgact gtgtggacat tgacagttat attctctacc 661 acctgctgga ggacagtgct ctcagagacc cgtggacgga caactgtctg ctgttggtca 721 ttgctaccag ggagtccatt cccgaagacc tgtaccagaa gttcatggcc tatctttctc 781 agggagggaa ggtgttgggc ctgtcttcat ccttcacctt tggtggcttt caggtgacaa 841 gcaagggtgc actgcacaag acagtccaga acttggtttt ctccaaggct gaccagagtg 901 aggtgaagct cagcgtcttg agcagtggct gcaggtacca ggaaggcccc gtccggctca 961 gccccggcag gctccagggc cacctggaga atgaggacaa ggacaggatg attgtgcatg 1021 tgccttttgg aactcgcggg ggagaagctg ttctttgcca ggtgcactta gaactacctc 1081 ccagctccaa catagtgcaa actccagaag attttaactt gctcaagtca agcaatttta 1141 gaagatacga agtccttaga gagattctga caacccttgg cctcagctgt gacatgaaac 1201 aagttcctgc cttaactcct ctttacttgc tgtcagctgc ggaggaaatc agggatcctc 1261 ttatgcagtg gcttgggaaa catgtggact ccgagggaga aataaaatcc ggccagctct 1321 ctcttagatt tgtttcatcc tacgtgtctg aagtagaaat aaccccatct tgtatacctg 1381 tggtgaccaa catggaggcc ttctcatcag aacatttcaa cttagagatc tatcgccaaa 1441 atctgcagac caagcagttg gggaaagtaa ttttgtttgc cgaagtgacc cccacaacga 1501 tgcgtctcct ggatgggctg atgtttcaga caccgcagga aatgggctta atagtgatcg 1561 cggcccggca gaccgagggc aaaggacggg gagggaatgt gtggctgagc cctgtgggat 1621 gtgctctttc tactctgctc atctccattc cactgagatc ccagctggga cagaggatcc 1681 cgtttgtcca gcatctgatg tccgtggctg tcgtggaagc agtgaggtcc attcccgagt 1741 atcaggatat caacttacga gtgaagtggc ccaacgatat ttattacagt gacctcatga 1801 agatcggcgg agttctggtt aactcaacac tcatgggaga aacattttat atacttattg 1861 gctgtggatt taatgtgact aacagtaacc ctaccatctg catcaacgac ctcatcacag 1921 aatacaataa acaacacaag gcagaactga agcccttaag agccgattat ctcatcgcca 1981 gagtcgtgac tgtgctggag aaactgatca aagagtttca ggacaaaggg cccaacagcg 2041 tccttcccct ttattaccga tactgggtcc acagtggtca gcaagtccat ctgggcagcg 2101 cagagggacc aaaggtgtcc atcgttggcc tggacgattc tggcttcctc caggttcacc 2161 aggagggcgg cgaggttgtg actgtgcacc cggacggcaa ctccttcgac atgctgagaa 2221 acctcatcct ccccaaacgg cggtacccaa ctttcttgta caaagtggtt ggtaagccta 2281 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 2341 gcgttaagtc gacaatcaac ctctggatta