Transcript: Human XM_011529539.3

PREDICTED: Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
HLCS (3141)
Length:
4843
CDS:
467..2647

Additional Resources:

NCBI RefSeq record:
XM_011529539.3
NBCI Gene record:
HLCS (3141)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011529539.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000045783 CCGCAGGAAATGGGCTTAATA pLKO.1 1934 CDS 100% 15.000 21.000 N HLCS n/a
2 TRCN0000233104 TGAAGTGGCCCAACGATATTT pLKO_005 2163 CDS 100% 15.000 21.000 N HLCS n/a
3 TRCN0000045786 CCTACGTGTCTGAAGTAGAAA pLKO.1 1740 CDS 100% 5.625 7.875 N HLCS n/a
4 TRCN0000233101 CAGACCTTCCCTACGATTATA pLKO_005 852 CDS 100% 15.000 12.000 N HLCS n/a
5 TRCN0000233103 AGTATCAGGATATCAACTTAC pLKO_005 2139 CDS 100% 10.800 7.560 N HLCS n/a
6 TRCN0000045787 GCCTGAACCTTCTCTTGAGAT pLKO.1 598 CDS 100% 4.950 3.465 N HLCS n/a
7 TRCN0000045785 GCTGTGGATTTAATGTGACTA pLKO.1 2262 CDS 100% 4.950 3.465 N HLCS n/a
8 TRCN0000045784 CCTACGATTATAGCAGCAGTT pLKO.1 861 CDS 100% 4.050 2.835 N HLCS n/a
9 TRCN0000233102 TACCTCCCAGCTCCAACATAG pLKO_005 1476 CDS 100% 0.000 0.000 N HLCS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011529539.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06380 pDONR223 100% 99.9% 100% None 834C>T n/a
2 ccsbBroad304_06380 pLX_304 0% 99.9% 100% V5 834C>T n/a
3 TRCN0000481180 TCCAGTAACTAACAAGCTTGTCTG pLX_317 20.9% 99.9% 100% V5 834C>T n/a
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