Construct: ORF ccsbBroad304_06516

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007982.1_s304c1, BRDN0000402317
Derived from:
ccsbBroadEn_06516
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to frame shift)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LASP1 (3927)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06516
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3927 LASP1 LIM and SH3 protein 1 NM_006148.4 99.6% 100% 357C>T;438A>G;783C>T
2 human 3927 LASP1 LIM and SH3 protein 1 NM_001271608.1 78.1% 68.6% (many diffs)
3 human 3927 LASP1 LIM and SH3 protein 1 NR_073384.2 19.1% (many diffs)
4 mouse 16796 Lasp1 LIM and SH3 protein 1 NM_010688.4 90.3% 95.4% (many diffs)
5 mouse 16796 Lasp1 LIM and SH3 protein 1 XM_006532341.1 90.3% 95.4% (many diffs)
6 mouse 16796 Lasp1 LIM and SH3 protein 1 XM_006532342.1 77.4% 82.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
849
ORF length:
783
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgaa ccccaactgc gcccggtgcg gcaagatcgt gtatcccacg gagaaggtga
121actgtctgga taagttctgg cataaagcat gcttccattg cgagacctgc aagatgacac
181tgaacatgaa gaactacaag ggctacgaga agaagcccta ctgcaacgca cactacccca
241agcagtcctt caccatggtg gcggacaccc cggaaaacct tcgcctcaag caacagagtg
301agctccagag tcaggtgcgc tacaaggagg agtttgagaa gaacaagggc aaaggtttca
361gcgtagtggc agacacgccc gagctccaga gaatcaagaa gacccaggac cagatcagta
421atataaaata ccatgaggag tttgagaaga gccgcatggg ccctagcggg ggcgagggca
481tggagccaga gcgtcgggat tcgcaggacg gcagcagcta ccggcggccc ctggagcagc
541agcagcctca ccacatcccg accagtgccc cggtttacca gcagccccag cagcagccgg
601tggcccagtc ctatggtggc tacaaggagc ctgcagcccc agtctccata cagcgcagcg
661ccccaggtgg tggcgggaag cggtaccgcg cggtgtatga ctacagcgcc gccgacgagg
721acgaggtctc cttccaggac ggggacacca tcgtcaacgt gcagcagatc gacgacggct
781ggatgtacgg gacggtggag cgcaccggcg acacggggat gctgccggcc aactacgtgg
841aggccatttg cccaacttct tgtacaaagt ggttggtaag cctatcccta accctctcct
901cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta agtcgacaat
961caacctctgg atta

Download FASTA (ORF) (Full)