Construct: ORF ccsbBroad304_06523
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004431.1_s304c1, BRDN0000462213
- Derived from:
- ccsbBroadEn_06523
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LIG1 (3978)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06523
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3978 | LIG1 | DNA ligase 1 | NM_000234.3 | 100% | 100% | |
| 2 | human | 3978 | LIG1 | DNA ligase 1 | NM_001320970.2 | 99.8% | 99.8% | 462_463insGAA |
| 3 | human | 3978 | LIG1 | DNA ligase 1 | NM_001320971.2 | 96.7% | 96.7% | 17_18ins90 |
| 4 | human | 3978 | LIG1 | DNA ligase 1 | NM_001289063.2 | 96.6% | 96.6% | 17_18ins90;372_373insGAA |
| 5 | human | 3978 | LIG1 | DNA ligase 1 | XM_024451513.1 | 96% | 96% | 466_467ins108 |
| 6 | human | 3978 | LIG1 | DNA ligase 1 | NM_001289064.1 | 92.6% | 92.4% | 370_371ins204 |
| 7 | human | 3978 | LIG1 | DNA ligase 1 | NR_135501.2 | 86% | (many diffs) | |
| 8 | human | 3978 | LIG1 | DNA ligase 1 | XR_002958316.1 | 75.9% | (many diffs) | |
| 9 | human | 3978 | LIG1 | DNA ligase 1 | XR_002958315.1 | 71.6% | 1_412del;1934_1935ins86;2684_2685ins399 | |
| 10 | human | 3978 | LIG1 | DNA ligase 1 | NR_135499.2 | 66.9% | (many diffs) | |
| 11 | human | 3978 | LIG1 | DNA ligase 1 | NR_135497.2 | 66.7% | 1_161del;2546_3548del;3922_4128del | |
| 12 | human | 3978 | LIG1 | DNA ligase 1 | NR_110296.2 | 66.7% | (many diffs) | |
| 13 | human | 3978 | LIG1 | DNA ligase 1 | NR_135498.2 | 64.7% | (many diffs) | |
| 14 | human | 3978 | LIG1 | DNA ligase 1 | NR_135500.2 | 62.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2826
- ORF length:
- 2757
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcagcgaagt atcatgtcat ttttccaccc caagaaagag ggtaaagcaa 121 agaagcctga gaaggaggca tccaatagca gcagagagac ggagccccct ccaaaggcgg 181 cactgaagga gtggaatgga gtggtgtccg agagtgactc tccggtgaag aggccaggga 241 ggaaggcggc ccgggtcctg ggcagcgaag gggaagagga ggatgaagcc cttagccctg 301 ctaaaggcca gaagcctgcc ctggactgct cacaggtctc cccgccccgt cctgccacat 361 ctcctgagaa caatgcttcc ctctctgaca cctctcccat ggacagttcc ccatcaggga 421 ttccgaagcg tcgcacagct cggaagcagc tcccgaaacg gaccattcag gaagtcctgg 481 aagagcagag tgaggacgag gacagagaag ccaagaggaa gaaggaggag gaagaagagg 541 agaccccgaa agaaagcctc acagaggctg aagtggccac agagaaggaa ggagaagacg 601 gggaccagcc caccacgcct cccaagcccc taaagacctc caaagcagag accccgacgg 661 aaagcgtttc agagcctgag gtggccacga agcaggaact gcaggaggag gaagagcaga 721 ccaagcctcc ccgcagagct cccaagacgc tcagcagctt cttcaccccc cggaagccag 781 cagtcaaaaa agaagtgaag gaagaggagc caggggctcc aggaaaggag ggagctgctg 841 agggacccct ggatccatct ggttacaatc ctgccaagaa caactatcat cccgtggaag 901 atgcctgctg gaaaccgggc cagaaggttc cttacctggc tgtggcccgg acgtttgaga 961 agatcgagga ggtgtctgct cggctccgga tggtggagac gctgagcaac ttgctgcgct 1021 ccgtggtggc cctgtcgcct ccagacctcc tccctgtcct ctacctcagc ctcaaccacc 1081 ttgggccacc ccagcagggc ctggagcttg gcgtgggtga tggtgtcctt ctcaaggcag 1141 tggcccaggc cacaggtcgg cagctggagt ccgtccgggc tgaggcagcc gagaaaggcg 1201 acgtggggct ggtggccgag aacagccgca gcacccagag gctcatgctg ccaccacctc 1261 cgctcactgc ctccggggtc ttcagcaagt tccgcgacat cgccaggctc actggcagtg 1321 cttccacagc caagaagata gacatcatca aaggcctctt tgtggcctgc cgccactcag 1381 aagcccggtt catcgctagg tccctgagcg gacggctgcg ccttgggctg gcagagcagt 1441 cggtgctggc tgccctctcc caggcagtga gcctcacgcc cccgggccaa gaattcccac 1501 cagccatggt ggatgctggg aagggcaaga cagcagaggc cagaaagacg tggctggagg 1561 agcaaggcat gatcctgaag cagacgttct gcgaggttcc cgacctggac cgaattatcc 1621 ccgtgctgct ggagcacggc ctggaacgtc tcccggagca ctgcaagctg agcccaggga 1681 ttcccctgaa accaatgttg gcccatccca cccggggcat cagcgaggtc ctgaaacgct 1741 ttgaggaggc agctttcacc tgcgaataca aatatgacgg gcagagggca cagatccacg 1801 ccctggaagg cggggaggtg aagatcttca gcaggaatca ggaagacaac actgggaagt 1861 acccggacat catcagccgc atccccaaga ttaaactccc atcggtcaca tccttcatcc 1921 tggacaccga agccgtggct tgggaccggg aaaagaagca gatccagcca ttccaagtgc 1981 tcaccacccg caaacgcaag gaggtggatg cgtctgagat ccaggtgcag gtgtgtttgt 2041 acgccttcga cctcatctac ctcaatggag agtccctggt acgtgagccc ctttcccggc 2101 gccggcagct gctccgggag aactttgtgg agacagaggg cgagtttgtc ttcgccacct 2161 ccctggacac caaggacatc gagcagatcg ccgagttcct ggagcagtca gtgaaagact 2221 cctgcgaggg gctgatggtg aagaccctgg atgttgatgc cacctacgag atcgccaaga 2281 gatcgcacaa ctggctcaag ctgaagaagg actaccttga tggcgtgggt gacaccctgg 2341 acctggtggt gatcggcgcc tacctgggcc gggggaagcg ggccggccgg tacgggggct 2401 tcctgctggc ctcctacgac gaggacagtg aggagctgca ggccatatgc aagcttggaa 2461 ctggcttcag tgatgaggag ctggaggagc atcaccagag cctcaaggcg ctggtgctgc 2521 ccagcccacg cccttacgtg cggatagatg gcgctgtgat tcccgaccac tggctggacc 2581 ccagcgctgt gtgggaggtg aagtgcgctg acctctccct ctctcccatc taccctgctg 2641 cgcggggcct ggtggatagt gacaagggca tctcccttcg cttccctcgg tttattcgag 2701 tccgtgaaga caagcagccg gagcaggcca ccaccagtgc tcaggtggcc tgtttgtacc 2761 ggaagcaaag tcagattcag aaccaacaag gcgaggactc aggctctgac cctgaagata 2821 cctacttgcc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 2881 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 2941 acctctggat ta