Construct: ORF ccsbBroad304_06523

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004431.1_s304c1, BRDN0000462213
Derived from:
ccsbBroadEn_06523
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LIG1 (3978)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06523
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3978 LIG1 DNA ligase 1 NM_000234.3 100% 100%
2 human 3978 LIG1 DNA ligase 1 NM_001320970.2 99.8% 99.8% 462_463insGAA
3 human 3978 LIG1 DNA ligase 1 NM_001320971.2 96.7% 96.7% 17_18ins90
4 human 3978 LIG1 DNA ligase 1 NM_001289063.2 96.6% 96.6% 17_18ins90;372_373insGAA
5 human 3978 LIG1 DNA ligase 1 XM_024451513.1 96% 96% 466_467ins108
6 human 3978 LIG1 DNA ligase 1 NM_001289064.1 92.6% 92.4% 370_371ins204
7 human 3978 LIG1 DNA ligase 1 NR_135501.2 86% (many diffs)
8 human 3978 LIG1 DNA ligase 1 XR_002958316.1 75.9% (many diffs)
9 human 3978 LIG1 DNA ligase 1 XR_002958315.1 71.6% 1_412del;1934_1935ins86;2684_2685ins399
10 human 3978 LIG1 DNA ligase 1 NR_135499.2 66.9% (many diffs)
11 human 3978 LIG1 DNA ligase 1 NR_135497.2 66.7% 1_161del;2546_3548del;3922_4128del
12 human 3978 LIG1 DNA ligase 1 NR_110296.2 66.7% (many diffs)
13 human 3978 LIG1 DNA ligase 1 NR_135498.2 64.7% (many diffs)
14 human 3978 LIG1 DNA ligase 1 NR_135500.2 62.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2826
ORF length:
2757
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gcagcgaagt atcatgtcat ttttccaccc caagaaagag ggtaaagcaa
121agaagcctga gaaggaggca tccaatagca gcagagagac ggagccccct ccaaaggcgg
181cactgaagga gtggaatgga gtggtgtccg agagtgactc tccggtgaag aggccaggga
241ggaaggcggc ccgggtcctg ggcagcgaag gggaagagga ggatgaagcc cttagccctg
301ctaaaggcca gaagcctgcc ctggactgct cacaggtctc cccgccccgt cctgccacat
361ctcctgagaa caatgcttcc ctctctgaca cctctcccat ggacagttcc ccatcaggga
421ttccgaagcg tcgcacagct cggaagcagc tcccgaaacg gaccattcag gaagtcctgg
481aagagcagag tgaggacgag gacagagaag ccaagaggaa gaaggaggag gaagaagagg
541agaccccgaa agaaagcctc acagaggctg aagtggccac agagaaggaa ggagaagacg
601gggaccagcc caccacgcct cccaagcccc taaagacctc caaagcagag accccgacgg
661aaagcgtttc agagcctgag gtggccacga agcaggaact gcaggaggag gaagagcaga
721ccaagcctcc ccgcagagct cccaagacgc tcagcagctt cttcaccccc cggaagccag
781cagtcaaaaa agaagtgaag gaagaggagc caggggctcc aggaaaggag ggagctgctg
841agggacccct ggatccatct ggttacaatc ctgccaagaa caactatcat cccgtggaag
901atgcctgctg gaaaccgggc cagaaggttc cttacctggc tgtggcccgg acgtttgaga
961agatcgagga ggtgtctgct cggctccgga tggtggagac gctgagcaac ttgctgcgct
1021ccgtggtggc cctgtcgcct ccagacctcc tccctgtcct ctacctcagc ctcaaccacc
1081ttgggccacc ccagcagggc ctggagcttg gcgtgggtga tggtgtcctt ctcaaggcag
1141tggcccaggc cacaggtcgg cagctggagt ccgtccgggc tgaggcagcc gagaaaggcg
1201acgtggggct ggtggccgag aacagccgca gcacccagag gctcatgctg ccaccacctc
1261cgctcactgc ctccggggtc ttcagcaagt tccgcgacat cgccaggctc actggcagtg
1321cttccacagc caagaagata gacatcatca aaggcctctt tgtggcctgc cgccactcag
1381aagcccggtt catcgctagg tccctgagcg gacggctgcg ccttgggctg gcagagcagt
1441cggtgctggc tgccctctcc caggcagtga gcctcacgcc cccgggccaa gaattcccac
1501cagccatggt ggatgctggg aagggcaaga cagcagaggc cagaaagacg tggctggagg
1561agcaaggcat gatcctgaag cagacgttct gcgaggttcc cgacctggac cgaattatcc
1621ccgtgctgct ggagcacggc ctggaacgtc tcccggagca ctgcaagctg agcccaggga
1681ttcccctgaa accaatgttg gcccatccca cccggggcat cagcgaggtc ctgaaacgct
1741ttgaggaggc agctttcacc tgcgaataca aatatgacgg gcagagggca cagatccacg
1801ccctggaagg cggggaggtg aagatcttca gcaggaatca ggaagacaac actgggaagt
1861acccggacat catcagccgc atccccaaga ttaaactccc atcggtcaca tccttcatcc
1921tggacaccga agccgtggct tgggaccggg aaaagaagca gatccagcca ttccaagtgc
1981tcaccacccg caaacgcaag gaggtggatg cgtctgagat ccaggtgcag gtgtgtttgt
2041acgccttcga cctcatctac ctcaatggag agtccctggt acgtgagccc ctttcccggc
2101gccggcagct gctccgggag aactttgtgg agacagaggg cgagtttgtc ttcgccacct
2161ccctggacac caaggacatc gagcagatcg ccgagttcct ggagcagtca gtgaaagact
2221cctgcgaggg gctgatggtg aagaccctgg atgttgatgc cacctacgag atcgccaaga
2281gatcgcacaa ctggctcaag ctgaagaagg actaccttga tggcgtgggt gacaccctgg
2341acctggtggt gatcggcgcc tacctgggcc gggggaagcg ggccggccgg tacgggggct
2401tcctgctggc ctcctacgac gaggacagtg aggagctgca ggccatatgc aagcttggaa
2461ctggcttcag tgatgaggag ctggaggagc atcaccagag cctcaaggcg ctggtgctgc
2521ccagcccacg cccttacgtg cggatagatg gcgctgtgat tcccgaccac tggctggacc
2581ccagcgctgt gtgggaggtg aagtgcgctg acctctccct ctctcccatc taccctgctg
2641cgcggggcct ggtggatagt gacaagggca tctcccttcg cttccctcgg tttattcgag
2701tccgtgaaga caagcagccg gagcaggcca ccaccagtgc tcaggtggcc tgtttgtacc
2761ggaagcaaag tcagattcag aaccaacaag gcgaggactc aggctctgac cctgaagata
2821cctacttgcc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
2881gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
2941acctctggat ta

Download FASTA (ORF) (Full)