Construct: ORF ccsbBroad304_06539

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001572.1_s304c1, BRDN0000409283
Derived from:
ccsbBroadEn_06539
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LSS (4047)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06539
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4047 LSS lanosterol synthase NM_001001438.2 99.9% 99.8% 864G>C;1924T>G
2 human 4047 LSS lanosterol synthase NM_002340.6 99.9% 99.8% 864G>C;1924T>G
3 human 4047 LSS lanosterol synthase NM_001145436.2 98.4% 98.3% 395_396ins33;831G>C;1891T>G
4 human 4047 LSS lanosterol synthase NM_001145437.1 88.9% 88.9% 0_1ins240;624G>C;1684T>G
5 mouse 16987 Lss lanosterol synthase NM_146006.2 82.9% 85.6% (many diffs)
6 mouse 16987 Lss lanosterol synthase XM_006513283.3 63.3% 65% (many diffs)
7 mouse 16987 Lss lanosterol synthase XM_017313817.1 58.8% 60.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2262
ORF length:
2196
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgac ggagggcacg tgtctgcggc gccgaggggg cccctacaag accgagcccg
121ccaccgacct cggccgctgg cgactcaact gcgagagggg ccggcagacg tggacctacc
181tgcaggacga gcgcgccggc cgcgagcaga ccggcctgga agcctacgcc ctggggctgg
241acaccaagaa ttactttaag gacttgccca aagcccacac cgcctttgag ggggctctga
301acgggatgac attttacgtg gggctgcagg ctgaggatgg gcactggacg ggtgattatg
361gtggcccact tttcctcctg ccaggcctcc tgatcacttg ccacgtggca cgcatccctc
421tgccagccgg atacagagaa gagattgtgc ggtacctgcg gtcagtgcag ctccctgacg
481gtggctgggg cctgcacatt gaggataagt ccaccgtgtt tgggactgcg ctcaactatg
541tgtctctcag aattctgggt gttgggcctg acgatcctga cctggtacga gcccggaaca
601ttcttcacaa gaaaggtggt gctgtggcca tcccctcctg ggggaagttc tggctggctg
661tcctgaatgt ttacagctgg gaaggcctca ataccctgtt cccagagatg tggctgtttc
721ctgactgggc accggcacac ccctccacac tctggtgcca ctgccggcag gtgtacctgc
781ccatgagcta ctgctacgcc gttcggctga gtgccgcgga agacccgctg gtccagagcc
841tccgccagga gctctatgtg gaggacttcg ccagcattga ctggctggcg cagaggaaca
901acgtggcccc cgacgagctg tacacgcccc acagctggct gctccgcgtg gtatatgcgc
961tcctcaacct gtatgagcac caccacagtg cccacctgcg gcagcgggcc gtgcagaagc
1021tgtatgaaca cattgtggcc gacgaccgat tcaccaagag catcagcatc ggcccgatct
1081cgaaaaccat caacatgctt gtgcgctggt atgtggacgg gcccgcctcc actgccttcc
1141aggagcatgt ctccagaatc ccggactatc tctggatggg ccttgacggc atgaaaatgc
1201agggcaccaa cggctcacag atctgggaca ccgcattcgc catccaggct ctgcttgagg
1261cgggcgggca ccacaggccc gagttttcgt cctgcctgca gaaggctcat gagttcctga
1321ggctctcaca ggtcccagat aaccctcccg actaccagaa gtactaccgc cagatgcgca
1381agggtggctt ctccttcagt acgctggact gcggctggat cgtttctgac tgcacggctg
1441aggccttgaa ggctgtgctg ctcctgcagg agaagtgtcc ccatgtcacc gagcacatcc
1501ccagagaacg gctctgcgat gctgtggctg tgctgctgaa catgagaaat ccagatggag
1561ggttcgccac ctatgagacc aagcgtgggg ggcacttgct ggagctgctg aacccctcgg
1621aggtcttcgg ggacatcatg attgactaca cctatgtgga gtgcacctca gccgtgatgc
1681aggcgcttaa gtatttccac aagcgtttcc cggagcacag ggcagcggag atccgggaga
1741ccctcacgca gggcttagag ttctgtcggc ggcagcagag ggccgatggc tcctgggaag
1801gctcctgggg agtttgcttc acctacggca cctggtttgg cctggaggcc ttcgcctgta
1861tggggcagac ctaccgagat gggactgcct gtgcagaggt ctcccgggcc tgtgacttcc
1921tgctgtcccg gcagatggca gacggaggct ggggggagga ctttgagtcc tgcgaggagc
1981ggcgttatgt gcagagtgcc cagtcccaga tccataacac atgctgggcc atgatggggc
2041tgatggccgt tcggcatcct gacatcgagg cccaggagag aggagtccgg tgtctacttg
2101agaaacagct ccccaatggc gactggccgc aggaaaacat tgctggggtc ttcaacaagt
2161cctgtgccat ctcctacacg agctacagga acatcttccc catctgggcc ctcggccgct
2221tctcccagct gtaccctgag agagcccttg ctggccaccc ctgcccaact ttcttgtaca
2281aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
2341ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

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