Construct: ORF ccsbBroad304_06602

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015587.1_s304c1, BRDN0000407164
Derived from:
ccsbBroadEn_06602
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MTM1 (4534)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06602
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4534 MTM1 myotubularin 1 NM_000252.2 99.9% 99.8% 1228G>A
2 human 4534 MTM1 myotubularin 1 XM_005274687.2 99.9% 99.8% 1228G>A
3 human 4534 MTM1 myotubularin 1 XM_011531173.2 99.9% 99.8% 1228G>A
4 human 4534 MTM1 myotubularin 1 XM_017029549.1 99.7% 99.6% 1228G>A;1644_1645insCAG
5 human 4534 MTM1 myotubularin 1 XM_011531171.1 97.5% 97.4% 231_275del;1273G>A
6 human 4534 MTM1 myotubularin 1 XM_011531172.1 97.5% 97.4% 231_275del;1273G>A
7 human 4534 MTM1 myotubularin 1 XM_017029548.1 97.5% 97.4% 231_275del;1273G>A
8 human 4534 MTM1 myotubularin 1 XM_017029547.1 97.3% 97.2% 231_275del;1273G>A;1689_1690insCAG
9 human 4534 MTM1 myotubularin 1 XM_017029550.1 93.8% 93.6% 231_232ins111;1117G>A
10 human 4534 MTM1 myotubularin 1 XM_017029551.2 58.8% 58.7% 0_1ins744;484G>A
11 mouse 17772 Mtm1 X-linked myotubular myopath... NM_001164190.1 88.7% 91.8% (many diffs)
12 mouse 17772 Mtm1 X-linked myotubular myopath... NM_001164191.1 88.7% 91.8% (many diffs)
13 mouse 17772 Mtm1 X-linked myotubular myopath... NM_019926.3 88.7% 91.8% (many diffs)
14 mouse 17772 Mtm1 X-linked myotubular myopath... NM_001164192.1 84.1% 86.8% (many diffs)
15 mouse 17772 Mtm1 X-linked myotubular myopath... XM_006527890.1 80.1% 82.5% (many diffs)
16 mouse 17772 Mtm1 X-linked myotubular myopath... XM_006527891.1 74.6% 78.1% (many diffs)
17 mouse 17772 Mtm1 X-linked myotubular myopath... XM_017318416.1 63.8% 65.9% (many diffs)
18 mouse 17772 Mtm1 X-linked myotubular myopath... NM_001164193.1 23.4% 21.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1875
ORF length:
1809
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ttctgcatca acttctaaat ataattcaca ctccttggag aatgagtcta
121ttaagaggac gtctcgagat ggagtcaatc gagatctcac tgaggctgtt cctcgacttc
181caggagaaac actaatcact gacaaagaag ttatttacat atgtcctttc aatggcccca
241ttaagggaag agtttacatc acaaattatc gtctttattt aagaagtttg gaaacggatt
301cttctctaat acttgatgtt cctctgggtg tgatctcgag aattgaaaaa atgggaggcg
361cgacaagtag aggagaaaat tcctatggtc tagatattac ttgtaaagac atgagaaacc
421tgaggttcgc tttgaaacag gaaggccaca gcagaagaga tatgtttgag atcctcacga
481gatacgcgtt tcccctggct cacagtctgc cattatttgc atttttaaat gaagaaaagt
541ttaacgtgga tggatggaca gtttacaatc cagtggaaga atacaggagg cagggcttgc
601ccaatcacca ttggagaata acttttatta ataagtgcta tgagctctgt gacacttacc
661ctgctctttt ggtggttccg tatcgtgcct cagatgatga cctccggaga gttgcaactt
721ttaggtcccg aaatcgaatt ccagtgctgt catggattca tccagaaaat aagacggtca
781ttgtgcgttg cagtcagcct cttgtcggta tgagtgggaa acgaaataaa gatgatgaga
841aatatctcga tgttatcagg gagactaata aacaaatttc taaactcacc atttatgatg
901caagacccag cgtaaatgca gtggccaaca aggcaacagg aggaggatat gaaagtgatg
961atgcatatca taacgccgaa cttttcttct tagacattca taatattcat gttatgcggg
1021aatctttaaa aaaagtgaag gacattgttt atcctaatgt agaagaatct cattggttgt
1081ccagtttgga gtctactcat tggttagaac atatcaagct cgttttgaca ggagccattc
1141aagtagcaga caaagtttct tcagggaaga gttcagtgct tgtgcattgc agtgacggat
1201gggacaggac tgctcagctg acatccttgg ccatgctgat gttggatagc ttctatagga
1261gcattgaagg gttcgaaata ctggtacaaa aaaaatggat aagttttgga cataaatttg
1321catctcgaat aggtcatggt gataaaaacc acaccgatgc tgaccgttct cctatttttc
1381tccagtttat tgattgtgtg tggcaaatgt caaaacagtt ccctacagct tttgaattca
1441atgaacaatt tttgattata attttggatc atctgtatag ttgccgattt ggtactttct
1501tattcaactg tgaatctgct cgagaaagac agaaggttac agaaaggact gtttctttat
1561ggtcactgat aaacagtaat aaagaaaaat tcaaaaaccc cttctatact aaagaaatca
1621atcgagtttt atatccagtt gccagtatgc gtcacttgga actctgggtg aattactaca
1681ttagatggaa ccccaggatc aagcaacaac agccgaatcc agtggagcag cgttacatgg
1741agctcttagc cttacgcgac gaatacataa agcggcttga ggaactgcag ctcgccaact
1801ctgccaagct ttctgatccc ccaacttcac cttccagtcc ttcgcaaatg atgccccatg
1861tgcaaactca cttctgccca actttcttgt acaaagtggt tggtaagcct atccctaacc
1921ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
1981cgacaatcaa cctctggatt a

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