Construct: ORF ccsbBroad304_06613

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015270.1_s304c1, BRDN0000408351
Derived from:
ccsbBroadEn_06613
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PPP1R12A (4659)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06613
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4659 PPP1R12A protein phosphatase 1 regul... NM_001143885.1 99.9% 99.9% 4A>C
2 human 4659 PPP1R12A protein phosphatase 1 regul... NM_002480.3 99.9% 99.9% 4A>C
3 human 4659 PPP1R12A protein phosphatase 1 regul... NM_001244990.1 96.5% 96.5% 4A>C;2800_2801ins105
4 human 4659 PPP1R12A protein phosphatase 1 regul... NM_001244992.1 94.5% 94.4% 4A>C;1653_1654ins168
5 human 4659 PPP1R12A protein phosphatase 1 regul... NM_001143886.1 91.5% 91.5% 0_1ins261
6 mouse 17931 Ppp1r12a protein phosphatase 1, regu... XM_006513326.3 89% 93.5% (many diffs)
7 mouse 17931 Ppp1r12a protein phosphatase 1, regu... XM_006513327.3 85.3% 89.4% (many diffs)
8 mouse 17931 Ppp1r12a protein phosphatase 1, regu... NM_027892.2 84.8% 88.3% (many diffs)
9 mouse 17931 Ppp1r12a protein phosphatase 1, regu... XM_006513328.3 84% 88.8% (many diffs)
10 mouse 17931 Ppp1r12a protein phosphatase 1, regu... XM_006513329.3 83.8% 88.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3159
ORF length:
3090
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gcagatggcg gacgcgaagc agaagcggaa cgagcagctg aaacgctgga
121tcggctccga gacggacctc gagcctccgg tggtgaagcg ccagaagacc aaggtgaagt
181tcgacgatgg cgccgtcttc ctggctgctt gctccagcgg cgacacggac gaggtcctca
241agctgctgca ccgcggcgcc gacatcaatt acgccaatgt ggacggactc actgccctgc
301accaggcttg cattgatgac aatgttgata tggtgaagtt tctggtagaa aatggagcaa
361atattaatca acctgataat gaaggctgga taccactaca tgcagcagct tcctgtggat
421atcttgatat tgcagagttt ttgattggtc aaggagcaca tgtaggggct gtcaacagtg
481aaggagatac acctttagat attgcggagg aggaggcaat ggaagagcta cttcaaaatg
541aagttaatcg gcaaggggtt gatatagaag cagctcgaaa ggaagaagaa cggatcatgc
601ttagagatgc caggcagtgg ctaaatagtg gtcatataaa tgatgtccgg catgcaaaat
661ctggaggtac agcacttcac gttgcagctg ctaaaggcta tacggaagtt ttaaaacttt
721taatacaggc aggctatgat gttaatatta aagactatga tggctggaca cctcttcatg
781ctgcagctca ttggggtaaa gaagaagcat gtcgaatttt agtggacaat ctgtgtgata
841tggagatggt caacaaagtg ggccaaacag cctttgatgt agcagatgaa gacattttag
901gatatttaga agagttgcaa aagaaacaaa atctgctcca tagtgaaaaa cgggacaaga
961aatctccact aattgaatca acagcaaata tggacaataa tcagtcacag aagaccttta
1021aaaacaaaga gacgttgatt attgaaccag agaaaaatgc atcccgtatt gaatctctgg
1081aacaagaaaa ggttgatgaa gaagaagaag gaaagaagga tgagtctagc tgctctagtg
1141aagaagatga ggaagatgac tcggaatcag aagctgaaac agataagaca aaacccctgg
1201cttctgtaac taatgccaac acttctagta cacaagcagc tcctgtagct gttacaacac
1261ctactgtgtc atcaggtcaa gcaacaccta catcacctat taaaaagttt ccaaccacag
1321ctacaaaaat ttctcccaaa gaagaagaga gaaaagatga gtctcctgca acttggaggt
1381taggacttag aaagacgggc agctatggtg cacttgctga aatcacagca tctaaagagg
1441gtcagaaaga aaaagatact gcaggtgtta cacgttcagc ttcaagtccc agactttcct
1501cctctttgga taataaagaa aaggagaaag atagtaaagg aactaggctt gcatatgttg
1561cacctacaat accaagacga ctagccagta catctgacat tgaagagaaa gaaaacagag
1621attcttcaag tttgcgaaca agtagttcat atacaaggag aaaatgggaa gatgatctta
1681aaaaaaatag ctcagttaat gaaggatcaa cgtatcataa aagttgctcc tttggtagaa
1741gacaagatga tttgattagt tctagtgttc caagcaccac atcaacacca acagttacct
1801ctgcagctgg gcttcagaaa agcctgcttt ccagcacaag cactactaca aagattacaa
1861cgggttcttc ctcagcaggc acacaaagca gtacctcaaa tcgtttgtgg gctgaggata
1921gtactgagaa agaaaaggac agtgttccta cggcagtgac cattcctgtt gctccaactg
1981ttgtaaatgc tgcagcttct accacaaccc tgactacaac tactgctggc actgtctcct
2041ccacaacaga ggtcagggag agacgcagat catacctcac tcctgttagg gatgaagagt
2101ctgaatccca aagaaaagca agatctagac aagcaagaca atctagaaga tcaacacagg
2161gagtgacatt aactgatctt caagaagctg agaaaacaat aggaagaagt cgttctaccc
2221gaaccagaga acaagaaaat gaagaaaaag aaaaagagga aaaagagaaa caagataaag
2281agaaacaaga agaaaagaag gagtcagaaa catctagaga agatgaatat aaacaaaagt
2341actccagaac gtatgatgag acttaccagc gttataggcc agtatcaact tcaagttcaa
2401ccactccatc ctcttcactt tctactatga gcagttcact gtatgcttca agtcaactaa
2461acaggccaaa tagtcttgta ggcataactt ctgcttactc cagaggaata acaaaagaaa
2521atgaaagaga gggagaaaaa agagaagagg agaaagaagg agaagataaa tcacaaccta
2581aatcaatcag agaacgacga cgaccaagag agaaaagaag atctacagga gtttcatttt
2641ggacacaaga tagtgatgaa aatgaacaag aacaacaatc agacacagaa gagggatcca
2701ataagaaaga aactcagacg gattccattt ctagatatga aaccagttct acatcagctg
2761gtgatcgata tgattccttg ctgggtcgct ctggatcata cagttactta gaagaaagaa
2821aaccttacag cagcaggcta gaaaaggatg actcaactga ctttaaaaag ctttatgaac
2881aaattctagc tgaaaatgaa aagctgaagg cacagctaca tgatacaaat atggaactaa
2941cagatcttaa attacagttg gaaaaggcca cccagagaca agaaagattt gctgatagat
3001cactgttgga aatggaaaaa agggaacgaa gagctctaga aagaagaata tctgaaatgg
3061aagaagagct caaaatgtta ccagacctaa aagcagacaa ccagaggcta aaggatgaaa
3121atggggcctt gatcagagtt ataagcaaac tttccaaatt gccaactttc ttgtacaaag
3181tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
3241gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.