Construct: ORF ccsbBroad304_06631
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003956.1_s304c1, BRDN0000408714
- Derived from:
- ccsbBroadEn_06631
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NELL1 (4745)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06631
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4745 | NELL1 | neural EGFL like 1 | NM_006157.5 | 99.9% | 99.8% | 245G>A;1857C>T |
| 2 | human | 4745 | NELL1 | neural EGFL like 1 | NM_001288713.1 | 96.5% | 96.5% | 54_137del;329G>A;1941C>T |
| 3 | human | 4745 | NELL1 | neural EGFL like 1 | NM_201551.2 | 94.1% | 94% | 245G>A;1644_1645ins141;1716C>T |
| 4 | human | 4745 | NELL1 | neural EGFL like 1 | NM_001288714.1 | 92.8% | 92.8% | 245G>A;331_332ins171;1686C>T |
| 5 | mouse | 338352 | Nell1 | NEL-like 1 | NM_001037906.2 | 87.8% | 93.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2499
- ORF length:
- 2430
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gccgatggat ttgattttag ttgtgtggtt ctgtgtgtgc actgccagga 121 cagtggtggg ctttgggatg gaccctgacc ttcagatgga tatcgtcacc gagcttgacc 181 ttgtgaacac cacccttgga gttgctcagg tgtctggaat gcacaatgcc agcaaagcat 241 ttttatttca agacatagaa agagagatcc atgcagctcc tcatgtgagt gagaaattaa 301 ttcagctgtt ccagaacaag agtgaattca ccattttggc cactgtacag cagaagccat 361 ccacttcagg agtgatactg tccattcgag aactggagca cagctatttt gaactggaga 421 gcagtggcct gagggatgag attcggtatc actacataca caatgggaag ccaaggacag 481 aggcacttcc ttaccgcatg gcagatggac aatggcacaa ggttgcactg tcagttagcg 541 cctctcatct cctgctccat gtcgactgta acaggattta tgagcgtgtg atagaccctc 601 cagataccaa ccttccccca ggaatcaatt tatggcttgg ccagcgcaac caaaagcatg 661 gcttattcaa agggatcatc caagatggga agatcatctt tatgccgaat ggatatataa 721 cacagtgtcc aaatctaaat cacacttgcc caacctgcag tgatttctta agcctggtgc 781 aaggaataat ggatttacaa gagcttttgg ccaagatgac tgcaaaacta aattatgcag 841 agacaagact tagtcaattg gaaaactgtc attgtgagaa gacttgtcaa gtgagtggac 901 tgctctatcg agatcaagac tcttgggtag atggtgacca ttgcaggaac tgcacttgca 961 aaagtggtgc cgtggaatgc cgaaggatgt cctgtccccc tctcaattgc tccccagact 1021 ccctcccagt gcacattgct ggccagtgct gtaaggtctg ccgaccaaaa tgtatctatg 1081 gaggaaaagt tcttgcagaa ggccagcgga ttttaaccaa gagctgtcgg gaatgccgag 1141 gtggagtttt agtaaaaatt acagaaatgt gtcctccttt gaactgctca gaaaaggatc 1201 acattcttcc tgagaatcag tgctgccgtg tctgtagagg tcataacttt tgtgcagaag 1261 gacctaaatg tggtgaaaac tcagagtgca aaaactggaa tacaaaagct acttgtgagt 1321 gcaagagtgg ttacatctct gtccagggag actctgccta ctgtgaagat attgatgagt 1381 gtgcagctaa gatgcattac tgtcatgcca atactgtgtg tgtcaacctt cctgggttat 1441 atcgctgtga ctgtgtccca ggatacattc gtgtggatga cttctcttgt acagaacacg 1501 atgaatgtgg cagcggccag cacaactgtg atgagaatgc catctgcacc aacactgtcc 1561 agggacacag ctgcacctgc aaaccgggct acgtggggaa cgggaccatc tgcagagctt 1621 tctgtgaaga gggctgcaga tacggtggaa cgtgtgtggc tcccaacaaa tgtgtctgtc 1681 catctggatt cacaggaagc cactgcgaga aagatattga tgaatgttca gagggaatca 1741 ttgagtgcca caaccattcc cgctgcgtta acctgccagg gtggtaccac tgtgagtgca 1801 gaagcggttt ccatgacgat gggacctatt cactgtccgg ggagtcctgt attgacattg 1861 atgaatgtgc cttaagaact cacacctgtt ggaacgattc tgcctgcatc aacctggcag 1921 ggggttttga ctgtctctgc ccctctgggc cctcctgctc tggtgactgt cctcatgaag 1981 gggggctgaa gcacaatggc caggtgtgga ccttgaaaga agacaggtgt tctgtctgct 2041 cctgcaagga tggcaagata ttctgccgac ggacagcttg tgattgccag aatccaagtg 2101 ctgacctatt ctgttgccca gaatgtgaca ccagagtcac aagtcaatgt ttagaccaaa 2161 atggtcacaa gctgtatcga agtggagaca attggaccca tagctgtcag cagtgtcggt 2221 gtctggaagg agaggtagat tgctggccac tcacttgccc caacttgagc tgtgagtata 2281 cagctatctt agaaggggaa tgttgtcccc gctgtgtcag tgacccctgc ctagctgata 2341 acatcaccta tgacatcaga aaaacttgcc tggacagcta tggtgtttca cggcttagtg 2401 gctcagtgtg gacgatggct ggatctccct gcacaacctg taaatgcaag aatggaagag 2461 tctgttgttc tgtggatttt gagtgtcttc aaaataattt gccaactttc ttgtacaaag 2521 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 2581 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta