Construct: ORF ccsbBroad304_06668
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005903.1_s304c1, BRDN0000462221
- Derived from:
- ccsbBroadEn_06668
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OCRL (4952)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06668
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4952 | OCRL | OCRL inositol polyphosphate... | NM_001587.4 | 99.9% | 99.8% | 1282T>G;2166A>G |
| 2 | human | 4952 | OCRL | OCRL inositol polyphosphate... | NM_000276.4 | 99% | 99% | 1282T>G;2115_2138del;2190A>G |
| 3 | human | 4952 | OCRL | OCRL inositol polyphosphate... | NM_001318784.2 | 98.9% | 98.8% | (many diffs) |
| 4 | mouse | 320634 | Ocrl | OCRL, inositol polyphosphat... | XM_017318524.2 | 88.5% | 91.3% | (many diffs) |
| 5 | mouse | 320634 | Ocrl | OCRL, inositol polyphosphat... | NM_177215.3 | 87.7% | 90.5% | (many diffs) |
| 6 | mouse | 320634 | Ocrl | OCRL, inositol polyphosphat... | XM_011251008.3 | 87.6% | 90.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2748
- ORF length:
- 2679
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagccgccg ctcccggtcg gagcccagcc gcttgccact gtcgagggta 121 tggagatgaa gggtcctctc cgggagccct gcgccctgac cctagcccag aggaacgggc 181 aatatgagtt aataatccag ttgcatgaga aggaacagca tgttcaagat atcattccta 241 taaatagcca cttcagatgt gttcaagaag cagaagaaac tcttttgatt gacatagctt 301 ctaacagtgg ctgcaaaatt cgggttcagg gggactggat cagagagcgc cgctttgaaa 361 tccctgatga ggaacactgt ttgaagttcc tctcagctgt ccttgctgct cagaaagctc 421 agtcacagct tcttgttcca gagcaaaagg actcatctag ctggtaccag aaattagaca 481 ctaaggacaa accttctgtt ttttcagggc ttcttggatt tgaagacaat ttttcttcta 541 tgaatttgga caagaaaata aattcacaaa atcagcctac tgggattcat cgggaacccc 601 cacctccacc cttttcagtg aataaaatgc ttccacgtga aaaagaagct tctaacaagg 661 agcagcccaa agtgaccaac accatgcgga agctctttgt accaaatacc caatctgggc 721 agcgggaggg tctcatcaaa catatcctgg caaagcgaga gaaagaatat gtcaacattc 781 agactttcag attttttgtt ggaacttgga atgtgaatgg ccagtctcca gatagcgggt 841 tagaaccttg gctgaactgt gatcccaatc ctcctgatat ctactgcatt ggattccaag 901 aactggactt gagcacagaa gccttcttct actttgaatc tgtgaaggaa caagaatggt 961 ccatggctgt agagagaggt ttgcattcca aagccaagta taagaaagtt caactggtgc 1021 gccttgttgg gatgatgctt cttatatttg ccagaaagga tcagtgtcga tacattcgtg 1081 atattgctac agaaacagtt ggaactggaa tcatggggaa aatgggaaac aaaggtgggg 1141 tagctgtgag atttgtattt cacaacacca ccttttgcat tgtcaattcc catctggctg 1201 cacacgtgga ggactttgag agaaggaatc aagattataa ggacatttgt gcgagaatga 1261 gttttgtggt cccaaatcag accctcccgc agttgaacat catgaaacat gaggttgtca 1321 tttggttggg agatttgaat tatagacttg gcatgcctga tgccaatgag gtgaaaagtc 1381 ttattaataa gaaagacctt cagagactct tgaaattcga ccagctaaat attcagcgca 1441 cacagaaaaa agcttttgtt gacttcaatg aaggggaaat caagttcatc cccacttata 1501 agtatgactc taaaacagac cggtgggatt ccagtgggaa atgccgggtt ccagcctggt 1561 gtgaccgaat tctttggaga ggaacaaatg ttaatcagct taattatcgg agtcacatgg 1621 aactgaaaac cagcgaccac aagcctgtta gcgccctctt ccatattggg gtgaaggttg 1681 tggatgaacg aaggtaccgg aaagtctttg aagatagtgt acgcatcatg gacagaatgg 1741 aaaatgactt ccttccttcc ttagaactca gcaggaggga gtttgtgttt gaaaatgtga 1801 agtttcggca actacaaaag gagaagttcc agatcagcaa caatggacag gttccctgcc 1861 atttttcttt catccctaaa cttaatgaca gccagtactg caagccatgg cttcgggctg 1921 aaccttttga gggctacttg gagccaaatg agacagtgga catttctctt gatgtgtatg 1981 tcagcaaaga ctctgtaacc atcctgaact cgggagaaga taagattgaa gatattctcg 2041 tccttcacct ggatcgaggc aaagattact tcttgactat cagtggaaat tacctcccaa 2101 gttgttttgg cacatcctta gaggctctgt gccgtatgaa aagaccaatc cgagaagttc 2161 ctgttaccaa actcatagac ttggagaaat cccttctgca aatggttcct ttggatgaag 2221 gtgccagtga gaggcccctt caggttccca aggagatctg gcttctagta gatcacctat 2281 tcaaatacgc ctgtcaccag gaggacctgt tccagacccc tggaatgcag gaagagctcc 2341 agcagatcat tgattgtctg gataccagca ttcctgagac aatccctggc agcaaccact 2401 ctgtggctga agcactgctc attttcttgg aagccctgcc agagccagtc atctgttacg 2461 agctgtatca gcgatgtctt gactctgctt atgatccccg gatctgccga caggtgatct 2521 cccagcttcc gagatgccat agaaatgttt tccgttactt gatggcattc cttcgagaac 2581 tcttaaaatt ctctgaatac aatagcgtca atgccaacat gatcgctact ctcttcacta 2641 gtcttctcct gaggcctcca cccaacctta tggcaagaca gactccaagt gaccgccagc 2701 gtgctattca gttccttctg ggctttctgc ttgggagcga agaagacttg ccaactttct 2761 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 2821 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta