Construct: ORF ccsbBroad304_06682
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015041.1_s304c1, BRDN0000400500
- Derived from:
- ccsbBroadEn_06682
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PABPC3 (5042)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06682
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5042 | PABPC3 | poly(A) binding protein cyt... | NM_030979.2 | 99.9% | 100% | 1224A>G |
| 2 | human | 26986 | PABPC1 | poly(A) binding protein cyt... | NM_002568.4 | 94.4% | 91.9% | (many diffs) |
| 3 | human | 26986 | PABPC1 | poly(A) binding protein cyt... | XM_005250861.3 | 94.4% | 91.9% | (many diffs) |
| 4 | human | 728773 | PABPC1P2 | poly(A) binding protein cyt... | NR_026904.1 | 38% | (many diffs) | |
| 5 | human | 113218505 | MIR10527 | microRNA 10527 | NR_162118.1 | 3.2% | (many diffs) | |
| 6 | mouse | 18458 | Pabpc1 | poly(A) binding protein, cy... | NM_008774.3 | 90.8% | 91.6% | (many diffs) |
| 7 | mouse | 18458 | Pabpc1 | poly(A) binding protein, cy... | XM_011245339.2 | 90.8% | 91.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1959
- ORF length:
- 1893
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa ccccagcacc cccagctacc caacggcctc gctctacgtg ggggacctcc 121 accccgacgt gactgaggcg atgctctacg agaagttcag cccggcaggg cccatcctct 181 ccatccggat ctgcagggac ttgatcacca gcggctcctc caactacgcg tatgtgaact 241 tccagcatac gaaggacgcg gagcatgctc tggacaccat gaattttgat gttataaagg 301 gcaagccagt acgcatcatg tggtctcagc gtgatccatc acttcgaaaa agtggagtgg 361 gcaacatatt cgttaaaaat ctggataagt ccattaataa taaagcactg tatgatacag 421 tttctgcttt tggtaacatc ctttcgtgta acgtggtttg tgatgaaaat ggttccaagg 481 gttatggatt tgtacacttt gagacacacg aagcagctga aagagctatt aaaaaaatga 541 acggaatgct cctaaatggt cgcaaagtat ttgttggaca atttaagtct cgtaaagaac 601 gagaagctga acttggagct agggcaaaag agttccccaa tgtttacatc aagaattttg 661 gagaagacat ggatgatgag cgccttaagg atctctttgg caagttcggg cccgccttaa 721 gtgtgaaagt aatgaccgat gaaagtggaa aatccaaagg atttggattt gtaagctttg 781 aaaggcatga agatgcacag aaagctgtag atgagatgaa tggaaaggag ctcaatggaa 841 aacaaattta cgttggtcga gctcagaaaa aagtggaacg gcagacggaa cttaagcgca 901 catttgaaca gatgaagcaa gataggatca ccagatacca ggttgttaat ctttatgtga 961 aaaatcttga tgatggtatt gatgatgaac gtctccggaa agcgttttct ccatttggta 1021 caatcactag tgcaaaggtt atgatggaag gtggtcgcag caaagggttt ggttttgtat 1081 gtttctcctc cccagaagaa gccactaaag cagttacaga aatgaacggt agaattgtgg 1141 ccacaaagcc attgtatgta gctttagctc agcgcaaaga agagcgccag gcttacctca 1201 ctaacgagta tatgcagaga atggcaagtg tacgagctgt gcccaaccag cgagcacctc 1261 cttcaggtta cttcatgaca gctgtcccgc agactcagaa ccatgctgca tactatcctc 1321 ctagccaaat tgctcgacta agaccaagtc ctcgctggac tgctcagggt gccagacctc 1381 atccattcca aaataagccc agtgctatcc gcccaggtgc tcctagagta ccatttagta 1441 ctatgagacc agcttcttca caggttccac gagtcatgtc aacgcagcgt gttgctaaca 1501 catcaacaca gacagtgggt ccacgtcctg cagctgctgc tgctgctgca gctacccctg 1561 ctgtgcgcac ggttccacgg tataaatatg ctgcgggagt tcgcaatcct cagcaacatc 1621 gtaatgcaca gccacaagtt acaatgcaac agcttgctgt tcatgtacaa ggtcaggaaa 1681 ctttgactgc ctccaggttg gcatctgccc ctcctcaaaa gcaaaagcaa atgttaggtg 1741 aacggctctt tcctcttatt caagccatgc accctactct tgctgggaaa atcactggca 1801 tgttgttgga gattgataat tcagaacttc tttatatgct cgagtctcca gagtcactcc 1861 gttctaaggt tgatgaagct gtagctgtac tacaagccca ccaagctaaa gaggctaccc 1921 agaaagcagt taacagtgct accggtgttc caactgttta cccaactttc ttgtacaaag 1981 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 2041 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta