Construct: ORF ccsbBroad304_06750
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015281.1_s304c1, BRDN0000408092
- Derived from:
- ccsbBroadEn_06750
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- POLR2H (5437)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06750
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5437 | POLR2H | RNA polymerase II subunit H | NM_006232.4 | 100% | 100% | |
| 2 | human | 5437 | POLR2H | RNA polymerase II subunit H | NM_001278698.2 | 85.7% | 69.6% | 336_399del;515_525del |
| 3 | human | 5437 | POLR2H | RNA polymerase II subunit H | XM_006713666.3 | 85.7% | 69.6% | 336_399del;515_525del |
| 4 | human | 5437 | POLR2H | RNA polymerase II subunit H | XM_006713667.2 | 85.7% | 69.6% | 336_399del;515_525del |
| 5 | human | 5437 | POLR2H | RNA polymerase II subunit H | NM_001278714.1 | 81.3% | 80.6% | 251_252ins84 |
| 6 | human | 5437 | POLR2H | RNA polymerase II subunit H | XM_017006636.2 | 81.3% | 80.6% | 251_252ins84 |
| 7 | human | 5437 | POLR2H | RNA polymerase II subunit H | NM_001278699.2 | 76% | 76% | 0_1ins108 |
| 8 | human | 5437 | POLR2H | RNA polymerase II subunit H | NM_001278700.1 | 76% | 76% | 0_1ins108 |
| 9 | human | 5437 | POLR2H | RNA polymerase II subunit H | NM_001346879.2 | 65.1% | 49.4% | 0_1ins108;228_291del;407_417del |
| 10 | human | 5437 | POLR2H | RNA polymerase II subunit H | XM_024453602.1 | 65.1% | 49.4% | 0_1ins108;228_291del;407_417del |
| 11 | human | 5437 | POLR2H | RNA polymerase II subunit H | NM_001278715.1 | 57.3% | 56.6% | 0_1ins108;143_144ins84 |
| 12 | human | 5437 | POLR2H | RNA polymerase II subunit H | XM_017006637.1 | 57.3% | 56.6% | 0_1ins108;143_144ins84 |
| 13 | mouse | 245841 | Polr2h | polymerase (RNA) II (DNA di... | NM_145632.2 | 92% | 100% | (many diffs) |
| 14 | mouse | 245841 | Polr2h | polymerase (RNA) II (DNA di... | XM_006522137.1 | 70.4% | 76% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 516
- ORF length:
- 450
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttgcgatggc gggcatcctg tttgaggata ttttcgatgt gaaggatatt gacccggagg 121 gcaagaagtt tgaccgagtg tctcgactgc attgtgagag tgaatctttc aagatggatc 181 taatcttaga tgtaaacatt caaatttacc ctgtagactt gggtgacaag tttcggttgg 241 tcatagctag taccttgtat gaagatggta ccctggatga tggtgaatac aaccccactg 301 atgataggcc ttccagggct gaccagtttg agtatgtaat gtatggaaaa gtgtacagga 361 ttgagggaga tgaaacttct actgaagcag caacacgcct ctctgcgtac gtgtcctatg 421 ggggcctgct catgaggctg cagggggatg ccaacaacct gcatggattc gaggtggact 481 ccagagttta tctcctgatg aagaagctag ccttctgaac cttcttgtac aaagtggttg 541 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 601 accggtggcg cgttaagtcg acaatcaacc tctggatta