Transcript: Human NM_001278715.1

Homo sapiens RNA polymerase II subunit H (POLR2H), transcript variant 6, mRNA.

Source:
NCBI, updated 2019-07-06
Taxon:
Homo sapiens (human)
Gene:
POLR2H (5437)
Length:
1189
CDS:
628..888

Additional Resources:

NCBI RefSeq record:
NM_001278715.1
NBCI Gene record:
POLR2H (5437)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001278715.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000053070 GCTAGTACCTTGTATGAAGAT pLKO.1 700 CDS 100% 4.950 6.930 N POLR2H n/a
2 TRCN0000286907 GCTAGTACCTTGTATGAAGAT pLKO_005 700 CDS 100% 4.950 6.930 N POLR2H n/a
3 TRCN0000294252 TCCACCGTGGCGGCATCTTTA pLKO_005 997 3UTR 100% 4.400 6.160 N POLR2H n/a
4 TRCN0000053068 GCATTGTGAGAGTGAATCTTT pLKO.1 603 5UTR 100% 5.625 4.500 N POLR2H n/a
5 TRCN0000053072 CGACTGCATTGTGAGAGTGAA pLKO.1 598 5UTR 100% 4.950 3.465 N POLR2H n/a
6 TRCN0000286908 CGACTGCATTGTGAGAGTGAA pLKO_005 598 5UTR 100% 4.950 3.465 N POLR2H n/a
7 TRCN0000111333 TGAGAGTGAATCTTTCAAGAT pLKO.1 609 5UTR 100% 4.950 3.465 N Polr2h n/a
8 TRCN0000332523 TGAGAGTGAATCTTTCAAGAT pLKO_005 609 5UTR 100% 4.950 3.465 N Polr2h n/a
9 TRCN0000053071 CCTGATGAAGAAGCTAGCCTT pLKO.1 864 CDS 100% 2.640 1.848 N POLR2H n/a
10 TRCN0000294251 TACCCTGGATGATGGTGAATA pLKO_005 723 CDS 100% 0.000 0.000 N POLR2H n/a
11 TRCN0000111331 GCCAACAACCTGCATGGATTT pLKO.1 820 CDS 100% 10.800 7.560 N Polr2h n/a
12 TRCN0000332520 GCCAACAACCTGCATGGATTT pLKO_005 820 CDS 100% 10.800 7.560 N Polr2h n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001278715.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06750 pDONR223 98.7% 57.3% 56.6% None 0_1ins108;143_144ins84 n/a
2 ccsbBroad304_06750 pLX_304 0% 57.3% 56.6% V5 (not translated due to prior stop codon) 0_1ins108;143_144ins84 n/a
3 TRCN0000475146 ACAGAAAAAGGGAAGGGTGTATCC pLX_317 57.4% 57.3% 56.6% V5 (not translated due to prior stop codon) 0_1ins108;143_144ins84 n/a
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