Construct: ORF ccsbBroad304_06760
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015195.1_s304c1, BRDN0000462328
- Derived from:
- ccsbBroadEn_06760
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPP2R3A (5523)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06760
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5523 | PPP2R3A | protein phosphatase 2 regul... | NM_002718.5 | 99.9% | 99.9% | 3302A>G |
| 2 | human | 5523 | PPP2R3A | protein phosphatase 2 regul... | XM_006713686.4 | 99.9% | 99.9% | 3302A>G |
| 3 | human | 5523 | PPP2R3A | protein phosphatase 2 regul... | XM_011512956.3 | 99.9% | 99.9% | 3302A>G |
| 4 | human | 5523 | PPP2R3A | protein phosphatase 2 regul... | XM_017006787.2 | 92.2% | 92.1% | 1992_1993ins267;3035A>G |
| 5 | human | 5523 | PPP2R3A | protein phosphatase 2 regul... | XR_001740196.2 | 69.7% | 1_589del;3221_3354del;3632_3633ins541 | |
| 6 | human | 5523 | PPP2R3A | protein phosphatase 2 regul... | NM_181897.3 | 43.8% | 42.5% | (many diffs) |
| 7 | human | 5523 | PPP2R3A | protein phosphatase 2 regul... | NM_001190447.2 | 35.9% | 35.9% | 0_1ins2208;1094A>G |
| 8 | mouse | 235542 | Ppp2r3a | protein phosphatase 2, regu... | NM_001161362.3 | 87.1% | 85.2% | (many diffs) |
| 9 | mouse | 235542 | Ppp2r3a | protein phosphatase 2, regu... | XM_017313347.1 | 87.1% | 85.2% | (many diffs) |
| 10 | mouse | 235542 | Ppp2r3a | protein phosphatase 2, regu... | XM_017313348.1 | 85% | 81.7% | (many diffs) |
| 11 | mouse | 235542 | Ppp2r3a | protein phosphatase 2, regu... | XM_017313349.1 | 84.6% | 82.2% | (many diffs) |
| 12 | mouse | 235542 | Ppp2r3a | protein phosphatase 2, regu... | XR_001778885.1 | 46.6% | (many diffs) | |
| 13 | mouse | 235542 | Ppp2r3a | protein phosphatase 2, regu... | NM_172144.3 | 40.2% | 38.8% | (many diffs) |
| 14 | mouse | 235542 | Ppp2r3a | protein phosphatase 2, regu... | XM_006511704.3 | 37.7% | 38.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3516
- ORF length:
- 3450
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc agcaacttac agacttgtgg ttagtactgt gaaccactac agcagcgtgg 121 tgatagaccg gcgttttgaa caagctatac attattgcac tggaacctgc cacaccttca 181 cacatggaat tgactgcatt gtggtacacc atagtgtttg tgcagacctc ttgcacatcc 241 ctgtgtctca gttcaaagat gcagatctga actctatgtt tctaccccat gaaaatgggc 301 tttcttcggc tgaaggagac tatccccaac aggccttcac aggcataccc agggtcaaga 361 gaggatctac atttcagaat acctacaact taaaggatat tgcaggagaa gcaatcagtt 421 ttgccagtgg gaaaataaaa gaattttcct ttgaaaaact caaaaactct aaccatgcag 481 cttacagaaa gggaaggaaa gttaagtctg actcatttaa taggaggtca gttgatttgg 541 acttgctttg tggccattat aacaacgatg ggaacgcccc atcctttggt ttactgcgga 601 gttcctcagt tgaggaaaaa cctttgtctc atagaaactc actggatacg aacctgactt 661 ccatgtttct tcaaaacttt tctgaagaag acttggttac tcagattttg gaaaaacata 721 aaatagataa tttttcttct gggacagaca taaagatgtg cttggacatc ttattgaaat 781 gctccgagga tttaaaaaaa tgcacagaca tcataaaaca atgcataaag aaaaaatcag 841 ggagtagcat cagtgaagga agtggtaatg atacaatttc tagctctgaa actgtctata 901 tgaatgtaat gaccaggtta gcatcctatc tgaaaaagtt accatttgaa ttcatgcagt 961 ctgggaataa tgaggctcta gatttaacag aactgatcag taatatgcct agcttacaac 1021 tgactccctt ctccccagtg tttggcactg aacaaccccc taaatatgaa gatgttgtcc 1081 agctctcagc ttctgactct ggacgatttc aaactattga attgcaaaat gacaagccta 1141 attctaggaa gatggacact gtacaatcca ttccaaacaa ctccacaaat tccttatata 1201 acttagaggt aaatgatcct agaactctaa aagctgtcca ggtccaatca cagtcattaa 1261 ccatgaatcc tttagaaaat gtttcttctg acgacttaat ggaaactctt tatattgaag 1321 aagagtcaga tggaaagaaa gcattagata aaggacaaaa gacagagaat ggacctagtc 1381 atgagttatt aaaggtaaat gaacatagag cagaatttcc agaacatgct actcatctta 1441 aaaaatgccc caccccaatg caaaatgaaa ttggtaagat atttgagaaa tcatttgtta 1501 atctacctaa ggaagactgt aaatcaaaag tttctaaatt tgaagaggga gaccagagag 1561 attttacaaa ttccagtagc caggaagaga tagataaatt gttaatggat ttggaatctt 1621 tttcacagaa gatggagacc tctctaagag agccacttgc gaagggtaaa aactctaatt 1681 ttttaaatag tcacagtcag ttgaccggtc agacccttgt agatcttgag cctaaatcta 1741 aagtctcttc acccatagaa aaagtctcac cttcctgtct aacaaggatt attgaaacca 1801 atggacacaa aatagaggaa gaggatcgag ccctcttact gcgaatcctg gaaagcattg 1861 aagactttgc tcaagaacta gttgaatgca aatcaagcag agggagccta tcacaagaaa 1921 aggaaatgat gcaaattcta caggaaacct tgacaacttc ctcccaggcc aatttatcag 1981 tctgtagaag tcctgttggt gataaagcca aagatactac ttcagcagtt ttgattcagc 2041 agactccaga ggtgatcaag attcaaaata aaccagaaaa gaaacctgga acaccactcc 2101 cacctccagc cacctctcca agtagtcccc gacctctctc cccggttccc catgtgaata 2161 atgttgtgaa tgcgccattg tccataaaca ttccacggtt ctactttcct gaaggactcc 2221 cagatacctg tagtaatcat gaacaaactc taagcagaat tgaaactgct ttcatggata 2281 ttgaagaaca gaaagcagac atttatgaaa tggggaaaat tgcaaaggtc tgtggctgtc 2341 ctctctattg gaaagccccc atgttcaggg ctgcaggggg agagaagaca ggatttgtga 2401 cagcacagtc attcattgcc atgtggagaa agttgctgaa taaccatcat gatgatgcct 2461 ctaaattcat ctgtcttcta gcaaagccca actgcagctc tctagaacag gaggatttca 2521 tccctctact tcaggatgtg gtggataccc accctggtct cacgttcctg aaagatgctc 2581 cagaattcca ctcccgctac atcaccacgg ttattcagag aatattctac acagtcaaca 2641 gatcttggag tggaaaaatt acttcgacag agataagaaa aagcaacttt ttgcaaaccc 2701 tagcactttt ggaagaagag gaagatataa accaaattac agattacttc tcctatgaac 2761 atttctatgt tatttattgt aaattctggg aactagatac tgatcacgac ctctacatca 2821 gccaggccga tctgtctcga tacaatgacc aggcttcatc aagcaggatt attgaaagga 2881 tattctctgg tgcagtaaca aggggaaaaa caatacagaa agagggaaga atgagctatg 2941 cagattttgt ttggtttttg atctctgaag aagacaaaag gaatcctacc agcattgagt 3001 attggttccg ctgcatggat gtggatggag acggtgtact ctccatgtat gagctggagt 3061 acttctatga ggagcagtgt gaacggatgg aagccatggg aattgagccc ttgccattcc 3121 atgatttact gtgccagatg cttgacctag tgaagccagc tgttgatggc aaaataactc 3181 taagagatct gaagaggtgc agaatggctc acatcttcta tgacactttc tttaatctgg 3241 agaaatactt agaccatgaa cagagagatc cctttgcggt ccagaaggat gttgagaacg 3301 atgggcctga gccctcagac tgggaccggt ttgccgctga ggagtatgag acgcttgttg 3361 cagagggatc tgcccaagca caattccagg aaggctttga agattatgaa acagatgaac 3421 ctgcctctcc ctctgaattt ggaaacaaaa gcaataaaat attaagtgca agccttccag 3481 agaaatgtgg aaagcttcaa tcagtggatg aagaataccc aactttcttg tacaaagtgg 3541 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 3601 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta