Construct: ORF ccsbBroad304_06826
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016487.1_s304c1, BRDN0000461647
- Derived from:
- ccsbBroadEn_06826
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PXN (5829)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06826
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5829 | PXN | paxillin | NM_002859.4 | 99.8% | 99.6% | 217A>G;1661A>G |
| 2 | human | 5829 | PXN | paxillin | XM_017019742.2 | 98.6% | 97.8% | (many diffs) |
| 3 | human | 5829 | PXN | paxillin | NM_001080855.3 | 94.1% | 93.9% | 217A>G;831_932del;1763A>G |
| 4 | human | 5829 | PXN | paxillin | XM_024449109.1 | 93.6% | 93.2% | (many diffs) |
| 5 | human | 5829 | PXN | paxillin | XM_017019740.2 | 93% | 92.2% | (many diffs) |
| 6 | human | 5829 | PXN | paxillin | NM_001243756.1 | 91.9% | 91.7% | 217A>G;830_973del;1805A>G |
| 7 | human | 5829 | PXN | paxillin | XM_017019739.2 | 90.9% | 90.1% | (many diffs) |
| 8 | human | 5829 | PXN | paxillin | XM_017019738.2 | 86.1% | 85.4% | (many diffs) |
| 9 | human | 5829 | PXN | paxillin | XM_017019737.2 | 79.3% | 78.7% | (many diffs) |
| 10 | human | 5829 | PXN | paxillin | NM_025157.5 | 76% | 75.9% | 0_1ins399;1262A>G |
| 11 | human | 5829 | PXN | paxillin | XM_017019743.1 | 76% | 75.9% | 0_1ins399;1262A>G |
| 12 | human | 5829 | PXN | paxillin | XM_017019736.2 | 74.2% | 73.6% | (many diffs) |
| 13 | human | 5829 | PXN | paxillin | XM_017019735.2 | 64.7% | 64.2% | (many diffs) |
| 14 | human | 5829 | PXN | paxillin | XM_005253917.3 | 64.1% | 52.3% | (many diffs) |
| 15 | human | 5829 | PXN | paxillin | XM_024449110.1 | 60.8% | 49.8% | (many diffs) |
| 16 | human | 5829 | PXN | paxillin | XM_017019733.2 | 59.1% | 58.6% | (many diffs) |
| 17 | human | 5829 | PXN | paxillin | XM_017019734.1 | 51.6% | 40.7% | (many diffs) |
| 18 | human | 5829 | PXN | paxillin | XM_017019729.2 | 51.1% | 50.6% | (many diffs) |
| 19 | human | 5829 | PXN | paxillin | XM_006719532.2 | 49.2% | 49.1% | 217A>G;830_2545del;3377A>G |
| 20 | human | 5829 | PXN | paxillin | XM_024449107.1 | 49% | 48.7% | (many diffs) |
| 21 | human | 5829 | PXN | paxillin | XM_024449108.1 | 49% | 48.8% | (many diffs) |
| 22 | human | 5829 | PXN | paxillin | XM_017019726.2 | 48.9% | 48.5% | (many diffs) |
| 23 | human | 5829 | PXN | paxillin | XM_017019730.2 | 41.9% | 41% | (many diffs) |
| 24 | human | 5829 | PXN | paxillin | XM_006719534.1 | 37.5% | 37.4% | 0_1ins399;431_2146del;2978A>G |
| 25 | human | 5829 | PXN | paxillin | XM_006719535.4 | 37.5% | 37.4% | 0_1ins399;431_2146del;2978A>G |
| 26 | human | 5829 | PXN | paxillin | XM_006719536.3 | 37.5% | 37.4% | 0_1ins399;431_2146del;2978A>G |
| 27 | human | 5829 | PXN | paxillin | XM_011538622.1 | 37.5% | 37.4% | 0_1ins399;431_2146del;2978A>G |
| 28 | human | 5829 | PXN | paxillin | XM_011538623.1 | 37.5% | 37.4% | 0_1ins399;431_2146del;2978A>G |
| 29 | human | 5829 | PXN | paxillin | XR_002957365.1 | 34.2% | (many diffs) | |
| 30 | mouse | 19303 | Pxn | paxillin | NM_011223.3 | 89.1% | 94.9% | (many diffs) |
| 31 | mouse | 19303 | Pxn | paxillin | NM_133915.3 | 84% | 89.5% | (many diffs) |
| 32 | mouse | 19303 | Pxn | paxillin | XM_006530204.2 | 46.1% | 37.6% | (many diffs) |
| 33 | mouse | 19303 | Pxn | paxillin | XM_006530205.2 | 46.1% | 37.6% | (many diffs) |
| 34 | mouse | 19303 | Pxn | paxillin | XM_006530202.3 | 45.6% | 48.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1740
- ORF length:
- 1671
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacgacctc gacgccctgc tggcggactt ggagtctacc acctcccaca 121 tctccaaacg gcctgtgttc ttgtcggagg agacccccta ctcataccca actggaaacc 181 acacatacca ggagattgcc gtgccacccc ccgtcccccc acccccgtcc agcgaggccc 241 tcaatggcac aatccttgac cccttagacc agtggcagcc cagcggctcc cgattcatcc 301 accagcagcc tcagtcctca tcacctgtgt acggctccag tgccaaaact tccagtgtct 361 ccaaccctca ggacagtgtt ggctctccgt gctcccgagt gggtgaggag gagcacgtct 421 acagcttccc caacaagcag aaatcagctg agccttcacc caccgtaatg agcacgtccc 481 tgggcagcaa cctttctgaa ctcgaccgcc tgctgctgga actgaacgct gtacagcata 541 acccgccagg cttccctgca gatgaggcca actcaagccc cccgcttcct ggggccctga 601 gccccctcta tggtgtccca gagactaaca gccccttggg aggcaaagct gggcccctga 661 cgaaagagaa gcctaagcgg aatgggggcc ggggcctgga ggacgtgcgg cccagtgtgg 721 agagtctctt ggatgaactg gagagctccg tgcccagccc cgtccctgcc atcactgtga 781 accagggcga gatgagcagc ccgcagcgcg tcacctccac ccaacagcag acacgcatct 841 cggcctcctc tgccaccagg gagctggacg agctgatggc ttcgctgtcg gatttcaagt 901 tcatggccca ggggaagaca gggagcagct caccccctgg ggggcccccg aagcccggga 961 gccagctgga cagcatgctg gggagcctgc agtctgacct gaacaagctg ggggtcgcca 1021 cagtcgccaa aggagtctgc ggggcctgca agaagcccat cgccgggcag gttgtgaccg 1081 ccatggggaa gacgtggcac cccgagcact tcgtctgcac ccactgccag gaggagatcg 1141 gatcccggaa cttcttcgag cgggatggac agccctactg tgaaaaggac taccacaacc 1201 tcttctcccc gcgctgctac tactgcaacg gccccatcct ggataaagtg gtgacagccc 1261 ttgaccggac gtggcaccct gaacacttct tctgtgcaca gtgtggagcc ttctttggtc 1321 ccgaagggtt ccacgagaag gacggcaagg cctactgtcg caaggactac ttcgacatgt 1381 tcgcacccaa gtgtggcggc tgcgcccggg ccatcctgga gaactatatc tcagccctca 1441 acacgctgtg gcatcctgag tgctttgtgt gccgggaatg cttcacgcca ttcgtgaacg 1501 gcagcttctt cgagcacgac gggcagccct actgtgaggt gcactaccac gagcggcgcg 1561 gctcgctgtg ttctggctgc cagaagccca tcaccggccg ctgcatcacc gccatggcca 1621 agaagttcca ccccgagcac ttcgtctgtg ccttctgcct caagcagctc aacaagggca 1681 ccttcaagga gcagaacgac aagccttact gtcagaactg cttcctcagg ctcttctgct 1741 tgccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 1801 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 1861 ggatta