Construct: ORF ccsbBroad304_06886
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001070.1_s304c1, BRDN0000461057
- Derived from:
- ccsbBroadEn_06886
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL36A (6173)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06886
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6173 | RPL36A | ribosomal protein L36a | NM_021029.6 | 100% | 100% | |
| 2 | human | 6166 | RPL36AL | ribosomal protein L36a like | NM_001001.5 | 89.9% | 99% | (many diffs) |
| 3 | human | 100529097 | RPL36A-HNRNPH2 | RPL36A-HNRNPH2 readthrough | NM_001199973.2 | 87.8% | 84.1% | (many diffs) |
| 4 | human | 100529097 | RPL36A-HNRNPH2 | RPL36A-HNRNPH2 readthrough | NM_001199974.2 | 66.2% | 60.3% | (many diffs) |
| 5 | mouse | 19982 | Rpl36a | ribosomal protein L36A | NM_019865.5 | 92.7% | 100% | (many diffs) |
| 6 | mouse | 66483 | Rpl36al | ribosomal protein L36A-like | NM_025589.4 | 87.7% | 100% | (many diffs) |
| 7 | mouse | 624713 | Gm6525 | predicted pseudogene 6525; ... | NR_036654.1 | 51% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 67
- ORF end:
- 385
- ORF length:
- 318
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggccatgg ttaacgtccc taaaacccgc cggactttct gtaagaagtg tggcaagcac 121 caaccccata aagtgacaca gtacaagaag ggcaaggatt ctctgtacgc ccagggaaag 181 cggcgttatg acaggaagca gagtggctat ggtgggcaaa ctaagccgat tttccggaaa 241 aaggctaaaa ctacaaagaa gattgtgcta aggcttgagt gcgttgagcc caactgcaga 301 tctaagagaa tgctggctat taaaagatgc aagcattttg aactgggagg agataagaag 361 agaaagggcc aagtgatcca gttcttgcca actttcttgt acaaagtggt tggtaagcct 421 atccctaacc ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg 481 cgcgttaagt cgacaatcaa cctctggatt a