Construct: ORF ccsbBroad304_07023
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018069.1_s304c1, BRDN0000402481
- Derived from:
- ccsbBroadEn_07023
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SYK (6850)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07023
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6850 | SYK | spleen associated tyrosine ... | NM_001135052.4 | 63.6% | 43.3% | (many diffs) |
| 2 | human | 6850 | SYK | spleen associated tyrosine ... | NM_001174168.3 | 63.6% | 43.3% | (many diffs) |
| 3 | human | 6850 | SYK | spleen associated tyrosine ... | NM_001174167.3 | 63.2% | 41.7% | (many diffs) |
| 4 | human | 6850 | SYK | spleen associated tyrosine ... | NM_003177.7 | 63.2% | 41.7% | (many diffs) |
| 5 | human | 6850 | SYK | spleen associated tyrosine ... | XM_005252147.4 | 63.2% | 41.7% | (many diffs) |
| 6 | human | 6850 | SYK | spleen associated tyrosine ... | XM_011518946.3 | 63.2% | 41.7% | (many diffs) |
| 7 | mouse | 20963 | Syk | spleen tyrosine kinase | XM_006516897.4 | 58.5% | 40.3% | (many diffs) |
| 8 | mouse | 20963 | Syk | spleen tyrosine kinase | XM_006516895.4 | 57.4% | 38.8% | (many diffs) |
| 9 | mouse | 20963 | Syk | spleen tyrosine kinase | XM_017315470.2 | 52.4% | 65.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 861
- ORF length:
- 795
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc cagcagcggc atggctgaca gcgccaacca cctgcccttc tttttcggca 121 acatcacccg ggaggaggca gaagattacc tggtccaggg gggcatgagt gatgggcttt 181 atttgctgcg ccagagccgc aactacctgg gtggcttcgc cctgtccgtg gcccacggga 241 ggaaggcaca ccactacacc atcgagcggg agctgaatgg cacctacgcc atcgccggtg 301 gcaggaccca tgccagcccc gccgacctct gccactacca ctcccaggag tctgatggcc 361 tggtctgcct cctcaagaag cccttcaacc ggccccaagg ggtgcagccc aagactgggc 421 cctttgagga tttgaaggaa aacctcatca gggaatatgt gaagcagaca tggaacctgc 481 agggtcaggc tctggagcag gccatcatca gtcagaagcc tcagctggag aagctgatcg 541 ctaccacagc ccatgaaaaa atgccttggt tccatggaaa aatctctcgg gaagaatctg 601 agcaaattgt cctgatagga tcaaagacaa atggaaagtt cctgatccga gccagagaca 661 acaacggctc ctacgccctg tgcctgctgc acgaagggaa ggtgctgcac tatcgcatcg 721 acaaagacaa gacagggaag ctctccatcc ccgagggaaa gaagttcgac acgctctggc 781 agctagtcga gcattattct tataaagcag atggtttgtt aagagttctt actgtcccat 841 gtcaaaaaat cggCACACAA TAGGGGGGTA GCTACCTGTA ATATAAAATG ATTTAAGTTA 901 TCTGATCCTG TCTGAAGGGA TGGGTGTAGC CGGTCCCACT CTTTGTCCAC AACCTTATGG 961 TGTTTTATAA CAGGGCCAGG AATTAATTCG AATCGGTTCA AGCTCCCTGC TTTCCCATAC 1021 TATATGTTTA AATTTATATT TTTCTTTCCC CCTGACCTGA CCGAATTTTT CCCATCGCGA 1081 TCTAATTCTC CCCCGCTTAA TACTGACGCT CTCGCACCCA TCTCTCTCCT TCTAGCCTCC 1141 GCTAGTCAAA AGTTTTGGAG TACTCACCAG TCGCCGCCCC TCGCCTCTTG CCGTGCGCGC 1201 TTCAGCAAGC CGAGTCCTGC GTCGAGAGAG CTCCTTTGGT TTCCCTTTCG CTTTCAAGTC 1261 CCTGTTCGGG CGCCAATGCT AGAGATTTTC CACACTGACT AAAAGGGTCT GAGGGATCTC 1321 TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT GAAGCACTCA AGGCAAGCTT 1381 TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA GCTCCCAGGC TCAGATCTGG 1441 TCTAACCAGA GAGACCCGTT TATGTATCGA GCTAGGCACT TAAATACAAT ATCTGTGCAA 1501 TGCGGCAATT CAGTGGTTCG TCCAATCCAT GTCAGACCCG TCAGTTGCCT TCCTAATAAG 1561 GCACGATCGT ACCACCTTAC TTCCACCAAT CGGCATGCAC GGTGCTTTTT CTCTCCTTGG 1621 AAGGCATGTT GCTAACTCAT CGTTACCATG TTGCAAGACT ACAAGAGTAA TGCATAAGAC 1681 TACATTAAGC TGGCAGCTCC AGCTTTTGTT CCCTTTAGTG AGGGTAAATG CGCGCTGGCG 1741 ATCATTACCC CGGcgtggtg aactacccaa ctttcttgta caaagtggtt ggtaagccta 1801 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 1861 gcgttaagtc gacaatcaac ctctggatta