Construct: ORF ccsbBroad304_07037
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002040.1_s304c1, BRDN0000400848
- Derived from:
- ccsbBroadEn_07037
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TCF12 (6938)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07037
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6938 | TCF12 | transcription factor 12 | NM_001322151.1 | 99.9% | 100% | 135A>G |
| 2 | human | 6938 | TCF12 | transcription factor 12 | NM_001322159.2 | 99.9% | 100% | 135A>G |
| 3 | human | 6938 | TCF12 | transcription factor 12 | NM_001322162.2 | 99.9% | 100% | 135A>G |
| 4 | human | 6938 | TCF12 | transcription factor 12 | NM_207036.2 | 99.9% | 100% | 135A>G |
| 5 | human | 6938 | TCF12 | transcription factor 12 | NM_207037.2 | 99.9% | 100% | 135A>G |
| 6 | human | 6938 | TCF12 | transcription factor 12 | NM_001322152.2 | 99.8% | 99.8% | 135A>G;1743_1744insAGC |
| 7 | human | 6938 | TCF12 | transcription factor 12 | NM_001322161.2 | 99.8% | 99.8% | 135A>G;1258_1259insAGC |
| 8 | human | 6938 | TCF12 | transcription factor 12 | XM_011521959.3 | 98.2% | 98.3% | 135A>G;149_184del |
| 9 | human | 6938 | TCF12 | transcription factor 12 | XM_011521960.3 | 98.2% | 98.3% | 135A>G;149_184del |
| 10 | human | 6938 | TCF12 | transcription factor 12 | XM_011521961.3 | 98.1% | 98.1% | 135A>G;149_184del;1779_1780insAGC |
| 11 | human | 6938 | TCF12 | transcription factor 12 | NM_001322157.2 | 96.5% | 96.6% | 135A>G;1187_1188ins72 |
| 12 | human | 6938 | TCF12 | transcription factor 12 | NM_001322165.2 | 96.5% | 96.6% | 135A>G;1187_1188ins72 |
| 13 | human | 6938 | TCF12 | transcription factor 12 | NM_003205.4 | 96.5% | 96.6% | 135A>G;1187_1188ins72 |
| 14 | human | 6938 | TCF12 | transcription factor 12 | NM_207038.2 | 96.5% | 96.6% | 135A>G;1187_1188ins72 |
| 15 | human | 6938 | TCF12 | transcription factor 12 | XM_017022520.2 | 96.4% | 96.4% | 135A>G;1187_1188ins72;1671_1672insAGC |
| 16 | human | 6938 | TCF12 | transcription factor 12 | NM_001322164.2 | 94.9% | 94.9% | 135A>G;149_184del;1223_1224ins72 |
| 17 | human | 6938 | TCF12 | transcription factor 12 | XM_011521962.3 | 94.9% | 94.9% | 135A>G;149_184del;1223_1224ins72 |
| 18 | human | 6938 | TCF12 | transcription factor 12 | XM_011521963.3 | 94.8% | 94.8% | (many diffs) |
| 19 | human | 6938 | TCF12 | transcription factor 12 | NM_001322156.2 | 90.4% | 88.6% | (many diffs) |
| 20 | human | 6938 | TCF12 | transcription factor 12 | NM_001322158.2 | 87% | 85.2% | (many diffs) |
| 21 | human | 6938 | TCF12 | transcription factor 12 | NM_001306219.3 | 74.6% | 72.1% | (many diffs) |
| 22 | human | 6938 | TCF12 | transcription factor 12 | NM_207040.2 | 71.2% | 68.7% | (many diffs) |
| 23 | human | 6938 | TCF12 | transcription factor 12 | XM_011521969.1 | 71.1% | 68.6% | (many diffs) |
| 24 | human | 6938 | TCF12 | transcription factor 12 | XM_011521965.2 | 69.4% | 58.6% | 0_1ins508;176_177ins140 |
| 25 | human | 6938 | TCF12 | transcription factor 12 | NM_001322154.2 | 68.9% | 68.9% | 0_1ins657 |
| 26 | human | 6938 | TCF12 | transcription factor 12 | XM_011521966.2 | 66.3% | 60.5% | (many diffs) |
| 27 | human | 6938 | TCF12 | transcription factor 12 | XM_011521967.2 | 66% | 55.4% | 0_1ins508;176_177ins140;539_540ins72 |
| 28 | human | 6938 | TCF12 | transcription factor 12 | NM_001306220.3 | 62.9% | 57.2% | (many diffs) |
| 29 | mouse | 21406 | Tcf12 | transcription factor 12 | NM_011544.3 | 91.5% | 95.6% | (many diffs) |
| 30 | mouse | 21406 | Tcf12 | transcription factor 12 | XM_006510994.3 | 91.5% | 95.6% | (many diffs) |
| 31 | mouse | 21406 | Tcf12 | transcription factor 12 | XM_006510995.2 | 91.3% | 95.4% | (many diffs) |
| 32 | mouse | 21406 | Tcf12 | transcription factor 12 | XM_006510996.2 | 91.3% | 95.4% | (many diffs) |
| 33 | mouse | 21406 | Tcf12 | transcription factor 12 | NM_001253862.1 | 88.1% | 92.2% | (many diffs) |
| 34 | mouse | 21406 | Tcf12 | transcription factor 12 | XM_006510997.2 | 88.1% | 92.2% | (many diffs) |
| 35 | mouse | 21406 | Tcf12 | transcription factor 12 | XM_006510998.2 | 87.9% | 92% | (many diffs) |
| 36 | mouse | 21406 | Tcf12 | transcription factor 12 | XM_006510999.2 | 87.9% | 92% | (many diffs) |
| 37 | mouse | 21406 | Tcf12 | transcription factor 12 | XM_006511000.3 | 83.3% | 84.4% | (many diffs) |
| 38 | mouse | 21406 | Tcf12 | transcription factor 12 | XM_017313270.1 | 79.9% | 81% | (many diffs) |
| 39 | mouse | 21406 | Tcf12 | transcription factor 12 | NM_001253863.1 | 68.5% | 68.6% | (many diffs) |
| 40 | mouse | 21406 | Tcf12 | transcription factor 12 | XM_006511002.1 | 68.3% | 66.2% | (many diffs) |
| 41 | mouse | 21406 | Tcf12 | transcription factor 12 | NM_001253864.1 | 65.1% | 65.2% | (many diffs) |
| 42 | mouse | 21406 | Tcf12 | transcription factor 12 | NM_001253865.1 | 59.7% | 62.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2184
- ORF length:
- 2118
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa tccccagcaa caacgcatgg ccgctatagg gaccgacaag gagctgagcg 121 acctactgga cttcagtgcg atgttttccc cacctgttaa tagtgggaaa actagaccaa 181 ctacactggg aagcagtcag ttcagtggat caggtattga tgaaagagga ggtacaacat 241 cttggggaac aagtggtcaa ccaagtcctt cctatgattc atctagaggt tttacagaca 301 gccctcatta cagtgatcac ttgaatgaca gtcgattagg agcccatgaa ggcttgtccc 361 caacaccttt catgaactca aatctgatgg gaaaaacatc agagagaggc tcattttccc 421 tgtacagcag agatactgga ttaccaggct gtcaatctag tctcctgaga caagatctgg 481 ggcttgggag cccagcacag ctatcttctt caggaaaacc tgggacagca tactattcat 541 tctctgctac aagttccagg aggagaccac tccatgactc tgcagcgctt gatcccttgc 601 aagcaaaaaa agtcagaaag gtgcctcctg gtttgccttc ttctgtatat gcaccatccc 661 caaattcaga tgatttcaac cgtgaatctc ctagttatcc atctcctaag ccaccaacca 721 gtatgttcgc tagcactttc tttatgcaag atgggaccca caattcttct gacctttgga 781 gttcatcaaa tgggatgagc cagcctggtt ttggtggaat tctggggacc tccacttccc 841 acatgtctca atccagtagt tatggcaacc ttcattcaca tgaccgcttg agttatcctc 901 cacactcagt ttcaccaaca gacataaaca cgagtcttcc accaatgtcc agctttcatc 961 gcggcagtac cagcagttca ccttacgttg ctgcctcaca cactcctccc atcaatggat 1021 cagacagcat tctaggaacc agagggaatg ctgctggaag ctcacagaca ggtgatgcac 1081 ttggaaaggc tttggcatct atttattctc ctgaccatac cagcagtagt tttccgtcaa 1141 atccatcaac accagttgga tcaccttcac ctctcacagg taccagtcag tggccaagac 1201 ctggagggca agcaccttca tccccaagct atgaaaactc actccactcc ctgaaaaatc 1261 gagttgagca gcaacttcac gagcatttgc aagatgcaat gtccttctta aaggatgtct 1321 gtgagcagtc tcgaatggag gatcgtttag acagactgga tgatgcaatc catgtgctgc 1381 ggaaccatgc tgtgggacct tccaccagtt tgcctgctgg tcacagtgat atacatagtt 1441 tattgggacc atcccataat gcaccaattg gaagcctcaa ttcaaactat ggaggatcaa 1501 gccttgttgc aagcagtcga tcagcttcaa tggttggaac tcatcgggaa gactctgtca 1561 gtctcaatgg caatcattca gtcctgtcta gtacagtcac tacttcaagc acagacctga 1621 accataaaac acaagaaaat tatagaggtg gcttgcaaag tcagtctgga actgttgtta 1681 caacagaaat caagactgaa aacaaagaaa aggatgaaaa ccttcatgaa cctccttcat 1741 cagatgacat gaagtcagat gatgaatcct cccaaaaaga tatcaaggtt tcatctagag 1801 gcagaacaag cagtactaat gaagatgagg atttgaaccc tgaacagaag atagaaaggg 1861 agaaggagag gcggatggct aacaatgcca gagaacgctt acgcgtgcgg gatattaatg 1921 aagcattcaa agagcttggc cgaatgtgtc agcttcactt gaagagtgaa aaaccccaaa 1981 caaaactcct tattcttcat caagccgtgg cagtcatcct tagtctagaa cagcaagtca 2041 gagagaggaa ccttaacccc aaagcagcct gccttaagag aagggaagaa gaaaaagttt 2101 ctgccgtatc ggcagagccg ccaaccacac tgccaggaac ccatcctggg cttagtgaaa 2161 ctaccaaccc tatgggtcat atgtacccaa ctttcttgta caaagtggtt ggtaagccta 2221 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 2281 gcgttaagtc gacaatcaac ctctggatta