Transcript: Human NM_001322154.2

Homo sapiens transcription factor 12 (TCF12), transcript variant 10, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
TCF12 (6938)
Length:
6109
CDS:
938..2401

Additional Resources:

NCBI RefSeq record:
NM_001322154.2
NBCI Gene record:
TCF12 (6938)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001322154.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000274222 CTTACGCGTGCGGGATATTAA pLKO_005 2113 CDS 100% 15.000 21.000 N TCF12 n/a
2 TRCN0000015171 CCATCCCATAATGCACCAATT pLKO.1 1664 CDS 100% 10.800 15.120 N TCF12 n/a
3 TRCN0000274168 CCATCCCATAATGCACCAATT pLKO_005 1664 CDS 100% 10.800 15.120 N TCF12 n/a
4 TRCN0000274170 CTCGAATGGAGGATCGTTTAG pLKO_005 1545 CDS 100% 10.800 15.120 N TCF12 n/a
5 TRCN0000274220 TACCAACCCTATGGGTCATAT pLKO_005 2377 CDS 100% 0.000 0.000 N TCF12 n/a
6 TRCN0000015168 GCTGTGATTATGGTGAACATA pLKO.1 3468 3UTR 100% 5.625 3.938 N TCF12 n/a
7 TRCN0000274221 GCTGTGATTATGGTGAACATA pLKO_005 3468 3UTR 100% 5.625 3.938 N TCF12 n/a
8 TRCN0000015169 CCCACAATTCTTCTGACCTTT pLKO.1 972 CDS 100% 4.950 3.465 N TCF12 n/a
9 TRCN0000015172 GCAATCATTCAGTCCTGTCTA pLKO.1 1785 CDS 100% 4.950 3.465 N TCF12 n/a
10 TRCN0000015170 CCACCTGTTAATAGTGGGAAA pLKO.1 370 5UTR 100% 4.050 2.835 N TCF12 n/a
11 TRCN0000075505 GCCGAATGTGTCAGCTTCATT pLKO.1 2154 CDS 100% 5.625 3.938 N Tcf12 n/a
12 TRCN0000075506 CCTTCATCAGATGACATGAAA pLKO.1 1949 CDS 100% 0.563 0.394 N Tcf12 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001322154.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07037 pDONR223 100% 68.9% 68.9% None 0_1ins657 n/a
2 ccsbBroad304_07037 pLX_304 0% 68.9% 68.9% V5 0_1ins657 n/a
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