Construct: ORF ccsbBroad304_07041
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014247.1_s304c1, BRDN0000399221
- Derived from:
- ccsbBroadEn_07041
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TCOF1 (6949)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07041
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6949 | TCOF1 | treacle ribosome biogenesis... | NM_001008657.3 | 99.9% | 99.8% | 919G>A |
| 2 | human | 6949 | TCOF1 | treacle ribosome biogenesis... | XM_005268508.4 | 96.2% | 95.7% | 919G>A;2855_2965del |
| 3 | human | 6949 | TCOF1 | treacle ribosome biogenesis... | XM_005268509.4 | 96% | 95.9% | (many diffs) |
| 4 | human | 6949 | TCOF1 | treacle ribosome biogenesis... | XM_017009795.2 | 91.9% | 91.8% | 637_638ins231;688G>A |
| 5 | human | 6949 | TCOF1 | treacle ribosome biogenesis... | XR_427779.2 | 80.2% | (many diffs) | |
| 6 | human | 6949 | TCOF1 | treacle ribosome biogenesis... | XR_427780.3 | 65.9% | (many diffs) | |
| 7 | human | 6949 | TCOF1 | treacle ribosome biogenesis... | NM_001195141.2 | 65.8% | 65.7% | (many diffs) |
| 8 | human | 6949 | TCOF1 | treacle ribosome biogenesis... | NM_001135244.2 | 65.7% | 65.7% | (many diffs) |
| 9 | human | 6949 | TCOF1 | treacle ribosome biogenesis... | XR_427778.3 | 64.8% | (many diffs) | |
| 10 | human | 6949 | TCOF1 | treacle ribosome biogenesis... | XM_017009792.2 | 64.2% | 64% | (many diffs) |
| 11 | human | 6949 | TCOF1 | treacle ribosome biogenesis... | XM_005268506.4 | 64.1% | 64% | (many diffs) |
| 12 | human | 6949 | TCOF1 | treacle ribosome biogenesis... | NM_001135243.1 | 64.1% | 64.1% | (many diffs) |
| 13 | human | 6949 | TCOF1 | treacle ribosome biogenesis... | NM_001371623.1 | 64% | 64% | (many diffs) |
| 14 | human | 6949 | TCOF1 | treacle ribosome biogenesis... | XM_005268503.4 | 62.6% | 62.5% | (many diffs) |
| 15 | human | 6949 | TCOF1 | treacle ribosome biogenesis... | XM_005268504.4 | 62.6% | 62.4% | (many diffs) |
| 16 | human | 6949 | TCOF1 | treacle ribosome biogenesis... | XM_005268502.4 | 62.5% | 62.4% | (many diffs) |
| 17 | human | 6949 | TCOF1 | treacle ribosome biogenesis... | XM_017009794.2 | 61.6% | 61.6% | (many diffs) |
| 18 | human | 6949 | TCOF1 | treacle ribosome biogenesis... | XM_017009793.2 | 60% | 60% | (many diffs) |
| 19 | human | 6949 | TCOF1 | treacle ribosome biogenesis... | NM_000356.4 | 58.9% | 58.9% | (many diffs) |
| 20 | human | 6949 | TCOF1 | treacle ribosome biogenesis... | NM_001135245.2 | 58.9% | 58.8% | (many diffs) |
| 21 | human | 6949 | TCOF1 | treacle ribosome biogenesis... | XM_011537678.3 | 58.6% | 58.5% | (many diffs) |
| 22 | human | 6949 | TCOF1 | treacle ribosome biogenesis... | XM_005268507.4 | 57.5% | 57.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2940
- ORF length:
- 2874
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc cgaggccagg aagcggcggg agctacttcc cctgatctac caccatctgc 121 tgcgggctgg ctatgtgcgt gcggcgcggg aagtgaagga gcagagcggc cagaagtgtt 181 tcctggctca gcccgtaacc cttctggaca tctatacaca ctggcaacaa acctcagagc 241 ttggtcggaa gcggaaggca gaggaagatg cggcactgca agctaagaaa acccgtgtgt 301 cagaccccat cagcacctcg gagagctcgg aagaggagga agaagcagaa gccgaaaccg 361 ccaaagccac cccaagacta gcatctacca actcctcagt cctgggggcg gacttgccat 421 caagcatgaa agaaaaagcc aaggcagaga cagagaaagc tggcaagact gggaattcca 481 tgccacaccc tgccactggg aagacggtgg ccaaccttct ttctgggaag tctcccagga 541 agtcagcaga gccctcagca aatactacgt tggtctcaga aactgaggag gagggcagcg 601 tcccggcctt tggagctgct gccaagcctg ggatggtgtc agcgggccag gccgacagct 661 ccagcgagga cacctccagc tccagtgatg agacagacgt ggaggggaaa ccctcagtaa 721 aaccagccca ggtcaaagcc tcatcagttt ctactaagga gtctccagca agaaaggcgg 781 ccccagcccc tgggaaggtg ggggatgtga caccccaggt caaaggaggg gccctgcccc 841 cagccaagag ggccaagaag ccagaagagg agtcagagag tagtgaggag ggatctgaaa 901 gtgaggagga ggcccctgca gggacacgaa gccaggtaaa ggcctctgaa aaaattctcc 961 aggtcagagc tgcctcagcc cctaccaagg ggacccctgg gaaaggggct accccagcac 1021 cccctgggaa ggcaggggct gtagcctccc agaccaaggc agggaagcca gaggaggact 1081 cagagagcag cagcgaggag tcatctgaca gtgaggagga gacgccagct gccaaggccc 1141 tgcttcaggc gaaggcctca ggaaaaacct ctcaggtcgg agctgcctca gcccctgcca 1201 aggagtcccc caggaaagga gctgccccag cgccccctgg gaagacaggg cctgcagttg 1261 ccaaggccca ggcggggaag cgggaggagg actcgcagag cagcagcgag gaatcggaca 1321 gtgaggagga ggcgcctgct caggcgaagc cttcagggaa ggccccccag gtcagagccg 1381 cctcggcccc tgccaaggag tcccccagga aaggggctgc cccagcacct cctaggaaaa 1441 cagggcctgc agccgcccag gtccaggtgg ggaagcagga ggaggactca agaagcagca 1501 gcgaggagtc agacagtgac agagaggcac tggcagccat gaatgcagct caggtgaagc 1561 ccttggggaa aagcccccag gtgaaacctg cctctaccat gggcatgggg cccttgggga 1621 aaggcgccgg cccagtgcca cccgggaagg tggggcctgc aaccccctca gcccaggtgg 1681 ggaagtggga ggaggactca gagagcagta gtgaggagtc atcagacagc agtgatggag 1741 aggtgcccac agctgtggcc ccggctcagg aaaagtcctt ggggaacatc ctccaggcca 1801 aacccacctc cagtcctgcc aaggggcccc ctcagaaggc agggcctgta gccgtccagg 1861 tcaaggctga aaagcccatg gacaactcgg agagcagcga ggagtcatcg gacagtgcgg 1921 acagtgagga ggcaccagca gccatgactg cagctcaggc aaaaccagct ctgaaaattc 1981 ctcagaccaa ggcctgccca aagaaaacca ataccactgc atctgccaag gtcgcccctg 2041 tgcgagtggg cacccaagcc ccccggaaag caggaactgc gacttctcca gcaggctcat 2101 ccccagctgt ggctgggggc acccagagac cagcagagga ttcttcaagc agtgaggaat 2161 cagatagtga ggaagagaag acaggtcttg cagtaaccgt gggacaggca aagtctgtgg 2221 ggaaaggcct ccaggtgaaa gcagcctcag tgcctgtcaa ggggtccttg gggcaaggga 2281 ctgctccagt actccctggg aagacggggc ctacagtcac ccaggtgaaa gctgaaaagc 2341 aggaagactc tgagagcagt gaggaggaat cagacagtga ggaagcagct gcatctccag 2401 cacaggtgaa aacctcagta aagaaaaccc aggccaaagc caacccagct gccgccagag 2461 caccttcagc aaaagggaca atttcagccc ctggaaaagt tgtcactgca gctgctcaag 2521 ccaagcagag gtctccatcc aaggtgaagc caccagtgag aaacccccag aacagtaccg 2581 tcttggcgag gggcccagca tctgtgccat ctgtggggaa ggccgtggct acagcagctc 2641 aggcccagac agggccagag gaggactcag ggagcagtga ggaggagtca gacagtgagg 2701 aggaggcgga gacgctggct caggtgaagc cttcagggaa gacccaccag atcagagctg 2761 ccttggctcc tgccaaggag tcccccagga aaggggctgc cccaacacct cctgggaaga 2821 cagggccttc ggctgcccag gcagggaagc aggatgactc agggagcagc agcgaggaat 2881 cagacagtga tggggaggca ccggcagctg tgacctctgc ccaggaccag gagtcttctt 2941 gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 3001 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 3061 ggatta