Construct: ORF ccsbBroad304_07070
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001352.1_s304c1, BRDN0000405417
- Derived from:
- ccsbBroadEn_07070
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TK2 (7084)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07070
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7084 | TK2 | thymidine kinase 2 | NM_004614.5 | 86.1% | 85.9% | 0_1ins126;34C>A;557C>A |
| 2 | human | 7084 | TK2 | thymidine kinase 2 | NM_001172645.1 | 80.2% | 80.1% | (many diffs) |
| 3 | human | 7084 | TK2 | thymidine kinase 2 | NM_001172644.2 | 77.9% | 77.8% | (many diffs) |
| 4 | human | 7084 | TK2 | thymidine kinase 2 | NM_001172643.1 | 73.7% | 73.2% | (many diffs) |
| 5 | human | 7084 | TK2 | thymidine kinase 2 | NM_001271934.2 | 70% | 69% | (many diffs) |
| 6 | human | 7084 | TK2 | thymidine kinase 2 | NM_001272050.2 | 54.6% | 54.3% | 0_1ins417;266C>A |
| 7 | human | 7084 | TK2 | thymidine kinase 2 | NM_001271935.1 | 45.5% | 35.6% | (many diffs) |
| 8 | human | 7084 | TK2 | thymidine kinase 2 | NR_073520.2 | 14.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 990
- ORF length:
- 921
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcgaccgggt ctctttaagg gccaggcgcc gggatccagg cggcgcccaa 121 cggctggact agcagtcgtc cgcgccgact cgcacaggaa ggaaccacgg gcctctggat 181 ccgctcgccc ggctatgctg ctgtggccgc tgcggggctg ggccgccagg gcgctgcgct 241 gctttgggcc gggaagtcgc gggagcccgg cctcaggccc cgggccgcgg agggtgcagc 301 gccgggcctg gcctcccgat aaagaacagg aaaaagagaa aaaatcagtg atctgtgtcg 361 agggcaatat tgcaagtggg aagacgacat gcctggaatt cttctccaac gcgacagacg 421 tcgaggtgtt aacggagcct gtgtccaagt ggagaaatgt ccgtggccac aatcctctgg 481 gcctgatgta ccacgatgcc tctcgctggg gtcttacgct acagacttat gtgcagctca 541 ccatgctgga caggcatact cgtcctcagg tgtcatctgt acggttgatg gagaggtcga 601 ttcacagcgc aagatacatt tttgtagaaa acctgtatag aagtgggaag atgccagaag 661 tggactatgt agttctgtcg gaatggtttg actggatctt gaggaacatg gacgtgtctg 721 ttgatttgat agtttacctt cggaccaatc atgagacttg ttaccagagg ttaaagaaga 781 gatgcaggga agaggagaag gtcattccgc tggaatacct ggaagcaatt caccatctcc 841 atgaggagtg gctcatcaaa ggcagccttt tccccatggc agcccctgtt ctggtgattg 901 aggctgacca ccacatggag aggatgttag aactctttga acaaaatcgg gatcgaatat 961 taactccaga gaatcggaag cattgcccat tgccaacttt cttgtacaaa gtggttggta 1021 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 1081 ggtggcgcgt taagtcgaca atcaacctct ggatta