Transcript: Human NR_073520.2

Homo sapiens thymidine kinase 2 (TK2), transcript variant 7, non-coding RNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
TK2 (7084)
Length:
5752
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_073520.2
NBCI Gene record:
TK2 (7084)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_073520.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000219751 TCGATTCACAGCGCAAGATAC pLKO.1 1392 3UTR 100% 10.800 15.120 N TK2 n/a
2 TRCN0000199783 GCGACAGACGTCGAGGTGTTA pLKO.1 1206 3UTR 100% 1.650 2.310 N TK2 n/a
3 TRCN0000350979 GCGACAGACGTCGAGGTGTTA pLKO_005 1206 3UTR 100% 1.650 2.310 N TK2 n/a
4 TRCN0000219750 ATCTGTGTCGAGGGCAATATT pLKO.1 1146 3UTR 100% 15.000 10.500 N TK2 n/a
5 TRCN0000006393 CCTGTGTCCAAGTGGAGAAAT pLKO.1 1233 3UTR 100% 13.200 9.240 N TK2 n/a
6 TRCN0000338422 CCTGTGTCCAAGTGGAGAAAT pLKO_005 1233 3UTR 100% 13.200 9.240 N TK2 n/a
7 TRCN0000219752 GTCTGTTGATTTGATAGTTTA pLKO.1 1511 3UTR 100% 13.200 9.240 N TK2 n/a
8 TRCN0000006392 CCTGTATAGAAGTGGGAAGAT pLKO.1 1427 3UTR 100% 4.950 3.465 N TK2 n/a
9 TRCN0000338423 CCTGTATAGAAGTGGGAAGAT pLKO_005 1427 3UTR 100% 4.950 3.465 N TK2 n/a
10 TRCN0000011017 GAGACTTGTTACCAGAGGTTA pLKO.1 1548 3UTR 100% 4.950 3.465 N TK2 n/a
11 TRCN0000350914 GAGACTTGTTACCAGAGGTTA pLKO_005 1548 3UTR 100% 4.950 3.465 N TK2 n/a
12 TRCN0000197174 GCTGTAAATAGAGGTAGCAAG pLKO.1 3132 3UTR 100% 4.050 2.835 N TK2 n/a
13 TRCN0000011015 CCACATCTTCATGTGGACATT pLKO.1 2521 3UTR 100% 0.495 0.347 N TK2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_073520.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07070 pDONR223 100% 14.6% None (many diffs) n/a
2 ccsbBroad304_07070 pLX_304 0% 14.6% V5 (many diffs) n/a
3 TRCN0000489689 AGCACTGAAGATCATTCCGTGCGT pLX_317 56.4% 11.9% V5 (not translated due to prior stop codon) (many diffs) n/a
4 ccsbBroadEn_11189 pDONR223 100% 11.2% None (many diffs) n/a
5 ccsbBroad304_11189 pLX_304 0% 11.2% V5 (many diffs) n/a
6 TRCN0000480376 CTCTATCCCCGCCTGTTCGCGTGC pLX_317 64.8% 11.2% V5 (many diffs) n/a
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