Construct: ORF ccsbBroad304_07088
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005407.1_s304c1, BRDN0000399961
- Derived from:
- ccsbBroadEn_07088
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TP53 (7157)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07088
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7157 | TP53 | tumor protein p53 | NM_000546.5 | 99.8% | 99.4% | 215C>G;832C>G |
| 2 | human | 7157 | TP53 | tumor protein p53 | NM_001126112.2 | 99.8% | 99.4% | 215C>G;832C>G |
| 3 | human | 7157 | TP53 | tumor protein p53 | NM_001126118.1 | 89.9% | 89.5% | 0_1ins117;98C>G;715C>G |
| 4 | human | 7157 | TP53 | tumor protein p53 | NM_001276760.2 | 89.9% | 89.5% | 0_1ins117;98C>G;715C>G |
| 5 | human | 7157 | TP53 | tumor protein p53 | NM_001276761.2 | 89.9% | 89.5% | 0_1ins117;98C>G;715C>G |
| 6 | human | 7157 | TP53 | tumor protein p53 | NM_001126113.2 | 85.9% | 84.4% | (many diffs) |
| 7 | human | 7157 | TP53 | tumor protein p53 | NM_001126114.2 | 84.5% | 83.9% | (many diffs) |
| 8 | human | 7157 | TP53 | tumor protein p53 | NM_001276695.2 | 76% | 74.5% | (many diffs) |
| 9 | human | 7157 | TP53 | tumor protein p53 | NM_001276696.2 | 74.7% | 74% | (many diffs) |
| 10 | human | 7157 | TP53 | tumor protein p53 | NM_001126115.1 | 66.3% | 66.1% | 0_1ins396;436C>G |
| 11 | human | 7157 | TP53 | tumor protein p53 | NM_001276697.2 | 59.4% | 59.2% | 0_1ins477;355C>G |
| 12 | human | 7157 | TP53 | tumor protein p53 | NM_001126117.1 | 52.6% | 51.1% | (many diffs) |
| 13 | human | 7157 | TP53 | tumor protein p53 | NM_001126116.1 | 51.3% | 50.6% | (many diffs) |
| 14 | human | 7157 | TP53 | tumor protein p53 | NM_001276699.2 | 45.7% | 44.2% | (many diffs) |
| 15 | human | 7157 | TP53 | tumor protein p53 | NM_001276698.2 | 44.5% | 43.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1245
- ORF length:
- 1179
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga ggagccgcag tcagatccta gcgtcgagcc ccctctgagt caggaaacat 121 tttcagacct atggaaacta cttcctgaaa acaacgttct gtcccccttg ccgtcccaag 181 caatggatga tttgatgctg tccccggacg atattgaaca atggttcact gaagacccag 241 gtccagatga agctcccaga atgccagagg ctgctccccg cgtggcccct gcaccagcag 301 ctcctacacc ggcggcccct gcaccagccc cctcctggcc cctgtcatct tctgtccctt 361 cccagaaaac ctaccagggc agctacggtt tccgtctggg cttcttgcat tctgggacag 421 ccaagtctgt gacttgcacg tactcccctg ccctcaacaa gatgttttgc caactggcca 481 agacctgccc tgtgcagctg tgggttgatt ccacaccccc gcccggcacc cgcgtccgcg 541 ccatggccat ctacaagcag tcacagcaca tgacggaggt tgTGAGGCGC TGCCCCCACC 601 ATGAGCGCTG CTCAGATAGC GATGGTCTGG CCCCTCCTCA GCATCTTATC CGAGTGGAAG 661 GAAATTTGCG TGTGGAGTAT TTGGATGACA GAAACACTTT TCGACATAGT GTGGTGGTGC 721 CCTATGAGCC GCCTGAGGTT GGCTCTGACT GTACCACCAT CCACTACAAC TACATGTGTA 781 ACAGTTCCTG CATGGGCGGC ATGAACCGGA GGCCCATCCT CACCATCATC ACACTGGAAG 841 ACTCCAGTGG TAATCTACTG GGACGGAACA GCTTTGAGGT GCGTGTTTGT GCCTGTGCTG 901 GGAGAGACCG GCGCACAGAG GAAGAGAATC TCCGCAAGAA AGGGGAGCCT CACCACGAGC 961 TGCCCCCAGG GAGCACTAAG CGAGCACTGC CCAACAACAC CAGCTCCTCT CCCCAGCCAA 1021 AGAAGAAACC ACTGGATGGA GAATATTTCA CCCTTCAGAT CCGTGGGCGT GAGCGCTTCG 1081 AGATGTTCCG AGAGCTGAAT GAGGCCTTGG AACTCAAGGA TGCCCAGGCT GGGAAGGAGC 1141 CAGGGGGGAG CAGGGCTCAC TCCAGCCACC TGAAGTCCAA AAAGGGTCAG TCTACCTCCC 1201 GCCATAAAAA ACTCATGTTC AAGACAGAAG GGCCTGACTC AGACTGCCCA ACTTTCTTGT 1261 ACaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 1321 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a