Construct: ORF ccsbBroad304_07430
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017620.1_s304c1, BRDN0000461533
- Derived from:
- ccsbBroadEn_07430
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TBX4 (9496)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07430
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9496 | TBX4 | T-box transcription factor 4 | NM_018488.3 | 99.8% | 99.6% | 17G>C;276T>G;587T>C |
| 2 | human | 9496 | TBX4 | T-box transcription factor 4 | NM_001321120.2 | 99.6% | 99.4% | (many diffs) |
| 3 | human | 9496 | TBX4 | T-box transcription factor 4 | XM_011525491.2 | 89.4% | 89.3% | (many diffs) |
| 4 | human | 9496 | TBX4 | T-box transcription factor 4 | XM_011525490.2 | 89.3% | 89.1% | (many diffs) |
| 5 | human | 9496 | TBX4 | T-box transcription factor 4 | XM_011525495.2 | 56.8% | 56.2% | (many diffs) |
| 6 | mouse | 21387 | Tbx4 | T-box 4 | NM_011536.2 | 87.9% | 92.3% | (many diffs) |
| 7 | mouse | 21387 | Tbx4 | T-box 4 | XM_006532780.2 | 87.9% | 92.3% | (many diffs) |
| 8 | mouse | 21387 | Tbx4 | T-box 4 | XM_006532779.2 | 85.7% | 90.1% | (many diffs) |
| 9 | mouse | 21387 | Tbx4 | T-box 4 | NM_172798.1 | 72.8% | 77.1% | (many diffs) |
| 10 | mouse | 21387 | Tbx4 | T-box 4 | XM_006532781.3 | 72.8% | 77.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1704
- ORF length:
- 1635
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gctgcaggat aaggccctgt ccgagagcga ggaggccttc cgggccccgg 121 gcccagcgct cggagaggcc agcgcagcca acgcccccga gcccgcgctg gcagcgccgg 181 gcctcagcgg agccgcgcta ggcagccccc cgggacccgg ggccgacgtc gtcgccgccg 241 ccgccgcgga gcagaccatc gagaacatca aggtggggct gcatgagaag gagctctgga 301 agaagttcca cgaggcgggc accgagatga tcatcactaa ggcgggcagg aggatgttcc 361 ccagctacaa ggtaaaagtc acaggcatga accccaagac caagtatatc ctgctgattg 421 acattgtccc tgccgatgac catcgctaca agttctgtga caacaaatgg atggtggcag 481 ggaaggctga gccagccatg ccaggaaggc tgtatgtcca cccggattct cctgccacag 541 gagcccactg gatgcggcag ctggtctcct tccagaagct gaagctgaca aacaaccacc 601 tggacccctt tggccatatc atcctcaact ctatgcacaa gtaccagccg cggccccaca 661 tcgttaaggc tgatgagaac aatgctttcg gctccaaaaa cactgctttc tgcacccacg 721 tgttcccaga gacctccttc atctctgtga cctcctacca gaatcacaag atcacccagc 781 tgaaaattga gaacaaccct tttgccaagg gattccgggg cagtgatgac agtgacctgc 841 gtgtggcccg actgcagagc aaagaatacc ccgtgatttc caaaagcatc atgaggcaga 901 ggctcatctc cccccagctc tcagccacac cggacgtggg ccccctgctc ggcacccacc 961 aggcactcca gcactaccag cacgagaacg gggcacactc acagctcgcg gagccgcagg 1021 acctgcccct cagcaccttt cccacccaga gggactcaag cctcttctat cactgcctga 1081 aaagacgaga cggtacccgc cacctggact taccttgcaa gcgatcctat ctggaagccc 1141 cctcttcggt gggggaggat cactatttcc gttccccccc tccctacgac cagcaaatgc 1201 tgagcccctc ctactgcagt gaggtgaccc ccagagaagc atgtatgtac tcaggttcag 1261 ggcccgagat tgccggggtg tctggggtgg acgacctgcc cccacctccg ctgagctgta 1321 acatgtggac ttcagtgtcg ccgtacacca gctatagcgt gcagacgatg gagactgtgc 1381 cgtaccagcc cttccccacg cacttcaccg ccaccaccat gatgccgcgg ctgcccaccc 1441 tctccgctca gagctcccag ccaccaggaa atgcccactt tagtgtctac aatcagctct 1501 cccagtctca ggtccgagag cgggggccca gcgcctcatt cccaagagag cgcggcctcc 1561 cccaagggtg tgagaggaag ccaccctcgc cacatctaaa tgctgccaat gagtttctct 1621 actctcaaac cttctccttg tcccgagaat cttccttaca gtaccattca ggaatgggga 1681 ctgtggagaa ctggactgac ggattgccaa ctttcttgta caaagtggtt ggtaagccta 1741 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 1801 gcgttaagtc gacaatcaac ctctggatta