Construct: ORF ccsbBroad304_07456

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005898.1_s304c1, BRDN0000404833
Derived from:
ccsbBroadEn_07456
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MICAL2 (9645)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07456
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9645 MICAL2 microtubule associated mono... NM_001282663.2 99.9% 99.9% 409C>A;414T>G
2 human 9645 MICAL2 microtubule associated mono... NM_001346292.2 99.9% 99.9% 409C>A;414T>G
3 human 9645 MICAL2 microtubule associated mono... NM_014632.4 99.9% 99.9% 409C>A;414T>G
4 human 9645 MICAL2 microtubule associated mono... NM_001282664.1 98% 98% 409C>A;414T>G;2782_2783ins63
5 human 9645 MICAL2 microtubule associated mono... NM_001346293.2 98% 98% 409C>A;414T>G;2782_2783ins63
6 human 9645 MICAL2 microtubule associated mono... NM_001346294.2 98% 98% 409C>A;414T>G;2782_2783ins63
7 human 9645 MICAL2 microtubule associated mono... NM_001346295.2 98% 98% 409C>A;414T>G;2782_2783ins63
8 human 9645 MICAL2 microtubule associated mono... NM_001346296.2 98% 98% 409C>A;414T>G;2782_2783ins63
9 human 9645 MICAL2 microtubule associated mono... NM_001346297.2 98% 98% 409C>A;414T>G;2782_2783ins63
10 human 9645 MICAL2 microtubule associated mono... NM_001346298.2 98% 98% 409C>A;414T>G;2782_2783ins63
11 human 9645 MICAL2 microtubule associated mono... NM_001346299.2 98% 98% 409C>A;414T>G;2782_2783ins63
12 human 9645 MICAL2 microtubule associated mono... NR_144415.2 85.7% (many diffs)
13 human 9645 MICAL2 microtubule associated mono... NR_144420.2 84.2% (many diffs)
14 human 9645 MICAL2 microtubule associated mono... NR_144418.2 84.1% (many diffs)
15 human 9645 MICAL2 microtubule associated mono... NR_144419.2 84.1% (many diffs)
16 human 9645 MICAL2 microtubule associated mono... NM_001282665.1 83% 83% 409C>A;414T>G;2212_2213ins570
17 human 9645 MICAL2 microtubule associated mono... NR_144416.2 82.6% (many diffs)
18 human 9645 MICAL2 microtubule associated mono... NR_144417.2 82.5% (many diffs)
19 human 9645 MICAL2 microtubule associated mono... NM_001282666.1 73.7% 73.7% (many diffs)
20 human 9645 MICAL2 microtubule associated mono... NM_001282667.1 72% 71.9% (many diffs)
21 human 9645 MICAL2 microtubule associated mono... NM_001282668.1 17.5% 17.3% (many diffs)
22 mouse 320878 Mical2 microtubule associated mono... NM_001193305.1 84.6% 86.2% (many diffs)
23 mouse 320878 Mical2 microtubule associated mono... XM_006507954.1 84.6% 86.2% (many diffs)
24 mouse 320878 Mical2 microtubule associated mono... NM_177282.5 62.8% 64.3% (many diffs)
25 mouse 320878 Mical2 microtubule associated mono... XR_001785575.1 44.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3438
ORF length:
3372
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggg ggaaaacgag gatgagaagc aggcccaggc ggggcaggtt tttgagaact
121ttgtccaggc atccacgtgc aaaggtaccc tccaggcctt caacattctc acacgacacc
181tggacctaga ccctctggac cacagaaact tttattccaa gctcaagtcc aaggtgacca
241cctggaaagc caaagccctg tggtacaaat tggataagcg tggttcccac aaagagtata
301agcgagggaa gtcgtgcacg aacaccaagt gtctcatagt tgggggagga ccctgtggct
361tgcgcactgc cattgaactt gcctacctgg gagccaaagt ggtcgtggtg gagaagaggg
421actccttctc ccggaacaac gtgctacacc tctggccttt caccatccat gacattcggg
481gcctgggagc caagaagttc tatgggaagt tctgtgctgg ctccatcgac catatcagta
541ttcgccaact acagctcatc ctattcaagg tggccctgat gctgggagtt gaaatccatg
601tgaatgtgga gttcgtgaag gttctagagc ctcctgaaga tcaagaaaat caaaaaattg
661gctggcgggc agaatttctc cctacagacc attctctgtc ggagtttgag tttgacgtca
721tcattggtgc cgatggccgc aggaacaccc tggaagggtt cagaagaaaa gaattccgtg
781ggaagctggc gattgccatc accgccaact tcataaacag aaacagcaca gcggaagcca
841aggtggaaga gattagtggt gtggctttca tcttcaatca gaaatttttt caggacctta
901aagaagaaac aggcatagat cttgagaaca ttgtttacta caaggactgc acccactatt
961ttgtaatgac agccaagaag cagagcctgc tcgacaaagg tgtcatcatt aacgactaca
1021tcgacacaga gatgctgctg tgtgcggaga acgtgaacca agacaacctg ctatcctatg
1081cccgggaagc tgcagacttt gccaccaact accagctgcc atccttagac tttgccatga
1141accactatgg gcagcctgat gtggccatgt ttgactttac ctgcatgtat gcctcagaga
1201acgcggccct ggtgcgggag cggcaggcgc accagctgct cgtggccctt gtgggtgaca
1261gcttgcttga gccattttgg cccatgggta caggctgtgc ccgtggcttc ctggcagcct
1321ttgacacggc atggatggtg aagagctgga accagggcac ccctcccctg gagctgctgg
1381ctgaaaggga aagtctctac cggctgttac ctcagacaac cccggagaac atcaacaaga
1441actttgagca gtacacgttg gacccaggga cacggtaccc aaacctcaac tcacactgtg
1501tcaggcccca tcaggtgaag catttgtata tcactaagga gctggagcac taccctctcg
1561agagactggg ctcggtgagg agatctgtca acctctccag gaaggagtca gatatccggc
1621ccagcaagct cctgacctgg tgccagcagc agacagaggg ctaccagcat gtcaacgtca
1681ccgacctgac cacatcctgg cgcagtgggt tggccctgtg tgccatcatc caccgcttcc
1741ggcctgagct catcaacttt gactctttga atgaagatga tgctgtggag aacaaccagc
1801tcgcatttga tgtggccgag cgagagtttg ggatccctcc agtgaccacg ggcaaagaga
1861tggcatctgc ccaggagcct gacaagctca gcatggtcat gtacctctcc aagttctacg
1921agctcttccg gggcacccca ctgaggcccg tggattcttg gcgcaaaaac tatggagaaa
1981atgctgacct cagcttggcc aaatcatcca tttctaataa ctatctcaac ctcacatttc
2041caaggaagag gactccacgg gtggatggtc aaaccggaga gaatgacatg aacaaacgga
2101gacggaaggg cttcaccaac ctggacgagc cttcaaactt ttccagccgt agcttgggct
2161ccaatcaaga gtgtgggagc agtaaggaag gtggaaatca gaacaaagtc aagtccatgg
2221cgaatcagct gctggccaag tttgaggaga gcactcggaa cccctcactc atgaagcagg
2281aacgccgtgt ctcagggata ggtaagccgg tcctgtgctc ttcctccggc cctcctgttc
2341actcttgctg ccccaagccg gaggaggcca cacccagccc atcacctcct ctgaaaaggc
2401agttcccctc tgtggtcgtg acggggcacg tgctcagaga gctcaagcaa gtgtctgctg
2461gcagtgagtg cctgagcaga ccttggagag ccagagccaa gtctgaccta cagctgggtg
2521ggacagaaaa tttcgctacc ctgccttcta cccgcccgag ggcgcaggct ctttccgggg
2581tgctgtggcg gctgcagcaa gtggaggaaa agattctcca gaagagggct cagaacttgg
2641ccaacaggga atttcacaca aagaacatta aggagaaggc ggctcacctt gcctccatgt
2701ttggacacgg ggatttcccg cagaataaac tactctctaa aggcctgtct catactcatc
2761ctccatctcc tccctctcgc cttccgtctc ctgatccagc tgcttcttcc tctccatcaa
2821ctgttgactc tgcttctcct gccagaaagg aaaagaagtc accttcaggg ttccattttc
2881atcccagcca tttgagaaca gtgcatcctc agctgacggt agggaaagtg tccagcggaa
2941taggggctgc agctgaagtc ctggtcaatc tgtacatgaa tgatcacaga cctaaggccc
3001aggccacctc tccagacctg gaatctatgc gaaagtcatt tccccttaac ctgggaggca
3061gcgacacgtg ttacttctgt aagaaacgtg tgtacgtgat ggaacggctg agcgccgagg
3121gccacttctt ccaccgggag tgtttccgct gcagcatctg tgccaccacc ttgcgcctgg
3181ccgcctacac ctttgactgc gatgaaggca aattttactg caagcctcac ttcattcact
3241gtaaaaccaa tagcaaacaa cggaagagac gggcagagtt gaagcaacaa agagaggagg
3301aggcaacatg gcaagagcag gaagcccctc ggagagacac tcccaccgaa agttcttgcg
3361cagtggccgc cattggcacc ctggaaggca gccccccagt tcatttcagc cttccagtgc
3421tacacccact tcttggctgc ccaactttct tgtacaaagt ggttggtaag cctatcccta
3481accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
3541agtcgacaat caacctctgg atta

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