Construct: ORF ccsbBroad304_07464
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003301.1_s304c1, BRDN0000462193
- Derived from:
- ccsbBroadEn_07464
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PHF14 (9678)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07464
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9678 | PHF14 | PHD finger protein 14 | NM_014660.4 | 99.8% | 99.8% | 344A>G;678G>A;1428T>C |
| 2 | human | 9678 | PHF14 | PHD finger protein 14 | NM_001007157.2 | 93.4% | 93.2% | (many diffs) |
| 3 | human | 9678 | PHF14 | PHD finger protein 14 | NR_033436.2 | 67.1% | (many diffs) | |
| 4 | human | 9678 | PHF14 | PHD finger protein 14 | NR_033435.2 | 19.6% | (many diffs) | |
| 5 | mouse | 75725 | Phf14 | PHD finger protein 14 | XM_006505200.3 | 90.7% | 96.2% | (many diffs) |
| 6 | mouse | 75725 | Phf14 | PHD finger protein 14 | NM_029404.3 | 90.5% | 96% | (many diffs) |
| 7 | mouse | 75725 | Phf14 | PHD finger protein 14 | XM_006505199.3 | 89.8% | 95.2% | (many diffs) |
| 8 | mouse | 75725 | Phf14 | PHD finger protein 14 | XM_006505198.3 | 88.7% | 94% | (many diffs) |
| 9 | mouse | 75725 | Phf14 | PHD finger protein 14 | XM_011241085.2 | 85.7% | 90.8% | (many diffs) |
| 10 | mouse | 75725 | Phf14 | PHD finger protein 14 | NM_001168382.1 | 84.8% | 89.8% | (many diffs) |
| 11 | mouse | 75725 | Phf14 | PHD finger protein 14 | XM_006505196.3 | 84.4% | 89.4% | (many diffs) |
| 12 | mouse | 75725 | Phf14 | PHD finger protein 14 | XM_006505197.3 | 84.4% | 89.4% | (many diffs) |
| 13 | mouse | 75725 | Phf14 | PHD finger protein 14 | XR_377294.3 | 71.6% | (many diffs) | |
| 14 | mouse | 75725 | Phf14 | PHD finger protein 14 | XR_377293.3 | 70.4% | (many diffs) | |
| 15 | mouse | 75725 | Phf14 | PHD finger protein 14 | XR_001785183.1 | 67.8% | (many diffs) | |
| 16 | mouse | 75725 | Phf14 | PHD finger protein 14 | XM_017321821.1 | 62.1% | 65.6% | (many diffs) |
| 17 | mouse | 75725 | Phf14 | PHD finger protein 14 | XM_017321819.1 | 58% | 61.1% | (many diffs) |
| 18 | mouse | 75725 | Phf14 | PHD finger protein 14 | XM_017321820.1 | 58% | 61.1% | (many diffs) |
| 19 | mouse | 75725 | Phf14 | PHD finger protein 14 | XM_006505201.3 | 57.7% | 60.8% | (many diffs) |
| 20 | mouse | 75725 | Phf14 | PHD finger protein 14 | XM_006505202.3 | 57.7% | 60.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2733
- ORF length:
- 2664
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatcgcagc tccaagagga ggcaggtgaa gcctttggca gcttctctgc 121 tggaagctct tgattatgat agttcagatg acagtgattt taaagttgga gatgcctcag 181 attctgaagg gagtggtaat ggaagtgaag atgcttcaaa ggacagtgga gaaggttcct 241 gtagtgattc tgaagaaaat attttagaag aagaactgaa tgaagatatt aaagtaaaag 301 aagaacaact taaaaattct gcagaggaag aagtactatc atcagaaaaa caattaatta 361 aaatggaaaa gaaggaagaa gaagaaaatg gagaaagacc tagaaagaaa agggagaaag 421 agaaggaaaa agaaaaggaa aaggagaaag agaaggaaag agagaaggaa aaagaaaaag 481 caacagtatc tgagaatgtg gctgcttctg ctgctgccac cacaccagcc acaagtcctc 541 ctgctgttaa cacatcccct tctgttccca ctacgacaac cgctacagag gaacaagtca 601 gcgagccaaa aaaatggaac cttcgacgaa accgaccact tctggatttt gtgtccatgg 661 aagagctgaa tgacatggat gactatgaca gtgaggatga caatgattgg cgacctactg 721 tagtaaagag aaaagggaga tctgcatctc agaaagaggg aagtgatgga gacaatgagg 781 atgatgaaga tgagggaagc gggagtgatg aagacgagaa tgatgaaggc aatgatgaag 841 atcatagtag ccctgccagt gaagggggtt gcaagaagaa gaagagtaaa gttcttagca 901 gaaacagtgc tgatgatgag gaactgacca atgatagcct gaccctatct caaagcaaga 961 gtaatgagga ctcgctgatt cttgagaaga gtcaaaactg gagctctcaa aaaatggacc 1021 atattctgat ttgctgtgtt tgtctgggag ataatagtga ggacgctgat gaaataattc 1081 agtgtgacaa ttgtggcatt acagtccatg aaggttgtta tggagttgat ggagagagtg 1141 actctattat gagttcagct tctgaaaact ccactgaacc ttggttttgt gatgcctgta 1201 aatgtggtgt ttctcctagc tgtgaactgt gtcctaatca ggatggaatt ttcaaggaga 1261 cagatgctgg aagatgggtt catattgttt gtgccctgta tgttcctgga gtagcctttg 1321 gagatattga caaattacga ccagtaacac taacggaaat gaactattcc aaatatggtg 1381 ccaaggagtg tagcttttgt gaagaccctc gctttgctag aactggggtt tgcattagct 1441 gtgatgcagg gatgtgcaga gcctatttcc atgtgacctg tgctcaaaag gaaggcctgc 1501 tttcagaggc agcggcggaa gaggatatag cagatccatt ctttgcttat tgtaagcaac 1561 atgcagatag gttagacaga aagtggaaga gaaaaaacta cttggctcta cagtcctatt 1621 gtaaaatgtc tttgcaagag agagagaagc aactatcacc agaagcacag gcaaggatca 1681 atgcccggct tcagcagtat cgtgccaaag cagaactagc tcgatctacc agaccccagg 1741 cctgggttcc aagggaaaaa ttgcccagac cactcaccag cagtgcttca gctattcgta 1801 aacttatgcg gaaagcagaa ctcatgggga tcagtacaga tatctttcca gtggacaatt 1861 cagatactag ttctagtgtg gatggaagga gaaaacataa gcaaccagct ctcactgcag 1921 attttgtgaa ttattatttt gagagaaata tgcgcatgat tcaaattcag gaaaatatgg 1981 ctgaacaaaa gaatataaaa gataaattag agaatgaaca agaaaagctt catgtagaat 2041 ataataagct atgtgaatct ttagaagaac tacaaaacct gaatggaaaa cttcgaagtg 2101 aaggacaagg aatatgggct ttactaggca gaatcacagg gcagaagttg aatataccgg 2161 caattttgcg agcacccaag gagagaaaac caagtaaaaa agaaggaggc acacaaaaga 2221 catctactct tcctgcagta ctttatagtt gtgggatttg taagaagaac catgatcagc 2281 atcttctttt attgtgtgat acctgtaaac tacattacca tcttggatgt ctggatcctc 2341 ctcttacaag gatgccaaga aagaccaaaa acagttattg gcagtgctcg gaatgtgacc 2401 aggcagggag cagtgacatg gaagcagata tggccatgga aaccctacca gatggaacca 2461 aacgatcaag gaggcagatt aaggaaccag tgaaatttgt tccacaggat gtgccaccag 2521 aacccaagaa gattccgata agaaacacga gaaccagagg acgaaaacga agcttcgttc 2581 ctgaggaaga aaaacatgag gaaagagttc ctagagagag aagacaaaga cagtctgtgt 2641 tgcaaaagaa gcccaaggct gaagatttaa gaactgaatg tgcaacttgc aagggaactg 2701 gagacaatga aaatcttgtc agataccctt cattgccaac tttcttgtac aaagtggttg 2761 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 2821 accggtggcg cgttaagtcg acaatcaacc tctggatta